A5087128482- MicroRNA in disease regulation
- Rheumatoid Arthritis Research and Therapies
- Cytokine Signaling Pathways and Interactions
- Nutrition and Health in Aging
- Adipose Tissue and Metabolism
- Frailty in Older Adults
- Immune cells in cancer
- Autoimmune and Inflammatory Disorders Research
- Systemic Sclerosis and Related Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle Physiology and Disorders
- Circular RNAs in diseases
- Telomeres, Telomerase, and Senescence
- Diabetes and associated disorders
- Wnt/β-catenin signaling in development and cancer
National Institute of Geriatrics, Rheumatology and Rehabilitation
2020-2025
Objectives The aim of our study was to determine whether there is a correlation between transcription factors expression and Th17/Treg ratio, cytokine profile in the RA phenotype as well identify that could be potential biomarker for RA. Methods conducted on 45 patients with RA, 27 OA 46 healthy controls (HCs). Th17 Treg frequency determined by flow cytometry (15 RA/OA 15 subjects HC). Gene estimated qPCR, serum levels were ELISA. Results percentage (CD4+CD25highCD127-) cells lower than or...
MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism genetic and epigenetic regulation Treg Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal was to understand potential impact selected microRNAs profiles on Treg/Th17 frequency, RA phenotype, profile microRNAs, their correlation with factors: SOCS1, SMAD3, SMAD4, STAT3, STAT5 in RA; we used...
Background Epigenetic factors influence inflammaging and geriatric disorders such as sarcopenia frailty. It is necessary to develop a biomarker/panel of biomarkers for fast easy diagnostics. Currently, hard-to-access equipment required diagnose sarcopenia. The development will prevent many older adults from being excluded the diagnostic process. Methods In this study, we analyzed selected gene expression profiles, namely, SIRT1 , SIRT3 SIRT6 DNMT3A FOXO1 FOXO3A ELAVL1 in whole blood. study...
Circulating free-cell miRNAs are increasingly important as potential non-invasive biomarkers due to the easy accessibility of clinical materials. Moreover, their epigenetic role may provide insight into mechanisms pathogenesis. Nevertheless, these aspects mostly studied in area oncological diseases. Therefore, this research aimed find association selected serum with expression Th17/Treg transcription factors and features RA patients. Accordingly, experiments was conducted on rheumatoid...
Aging is a biological event that influences many organs and systems. Both sarcopenia frailty syndrome refer to geriatric conditions with overlapping phenotypes. Many mechanisms are involved in the aging process such as DNA methylation telomeres which susceptible oxidative stress, inflammations result telomere shortening, leading chromosomal instability. The study aimed determine associations between these processes, syndrome. Global was analyzed using ELISA method. Telomere length qPCR....
Abstract Objectives The aim of this research was to prospectively evaluate the impact NOX2 gene expression profile (including NCF1 , NCF2 and NCF4 genes) in peripheral blood mononuclear cells (PBMCs) on immune signatures, clinical characteristics responsiveness anti-TNFα treatment RA patients. Methods Blood specimens were collected from rheumatoid arthritis (RA) patients healthy control pairs before anti-TNF 16 followed at two timepoints (months 3 6) populations determined based mRNA nine...
Mixed connective tissue disease (MCTD) is a very rare disorder that belongs in the and clinically multifactorial groups of diseases. The pathogenesis MCTD still unclear. best understood epigenetic alteration DNA methylation whose role to regulate gene expression. In literature, there are ever-increasing assumptions can be one possible reasons for development Autoimmune Connective Tissue Diseases (ACTDs) such as systemic sclerosis (SSc) lupus erythematosus (SLE). aim this study was define...
Prostaglandin signaling pathways are closely related to inflammation, but also muscle regeneration and processes associated with frailty sarcopenia, whereas β-catenin (CTNNB1 gene) as a part of Wnt is involved in the differentiation cells fibrosis. The present study analyzed association between selected prostaglandin pathway genes clinical parameters patients sarcopenia syndrome. was conducted on syndrome, control older (N = 25). Additionally, two healthy controls at age 25–30 years 51)...