A5062742783- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Protein Structure and Dynamics
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Cancer Treatment and Pharmacology
- Crystallization and Solubility Studies
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Biochemical and Molecular Research
- Microfluidic and Capillary Electrophoresis Applications
- Manufacturing Process and Optimization
- Design Education and Practice
- Plant and Fungal Interactions Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Acute Lymphoblastic Leukemia research
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Hematological disorders and diagnostics
- 3D Printing in Biomedical Research
- Hemoglobin structure and function
- Photosynthetic Processes and Mechanisms
Architecture et Réactivité de l'arN
2015-2025
Université de Strasbourg
2014-2025
Centre National de la Recherche Scientifique
2014-2025
Institut de Biologie Moléculaire et Cellulaire
2013-2024
European X-Ray Free-Electron Laser
2023
Brookhaven National Laboratory
2023
Institut de Biologie Moléculaire des Plantes
1999-2018
Karlsruhe Institute of Technology
2006-2009
The University of Tokyo
2009
Université Paris-Saclay
2008
The Hfq protein was discovered in Escherichia coli the early seventies as a host factor for Qbeta phage RNA replication. During last decade, it shown to be involved many processing events and remote sequence homology indicated link spliceosomal Sm proteins. We report crystal structure of E.coli showing that its monomer displays characteristic Sm-fold forms homo-hexamer, agreement with former biochemical data. Overall, ring is similar one recently described Staphylococcus aureus. This...
The bacteriophage phi29 DNA packaging motor, one of the strongest biological motors characterized to date, is geared by a RNA (pRNA) ring. When assembled from three fragments, its three-way junction (3WJ) motif highly thermostable, resistant 8 M urea, and remains associated at extremely low concentrations in vitro vivo. To elucidate structural basis for unusual stability, we solved crystal structure this pRNA 3WJ 3.05 Å. revealed two divalent metal ions that coordinate 4 nt fragments....
Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search optimization crystallization conditions by counter-diffusion, as well on-chip analysis crystals X-ray diffraction. Chips composed microchannels fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) cyclo-olefin-copolymer (COC) three distinct methods, namely replica casting, laser ablation hot embossing. The geometry channels was chosen ensure that occurs a...
The Sm proteins are conserved in all three domains of life and always associated with U-rich RNA sequences. Their proposed function is to mediate RNA-RNA interactions. We present here the crystal structures Pyrococcus abyssi protein (PA-Sm1) its complex a uridine heptamer. overall structure complex, heptameric ring central cavity, similar that for eukaryotic core found other archaeal proteins. molecules bind at two different sites. They interact specifically inside highly residues, defining...
RNase P is the essential activity removing 5'-leader sequences from transfer RNA precursors. was always associated with ribonucleoprotein complexes before discovery of protein-only enzymes called PRORPs (PROteinaceous P) in eukaryotes. Here we provide biophysical and functional data to understand mode action PRORP enzymes. Activity assays footprinting experiments show that anticodon domain dispensable, whereas individual residues D TψC loops are for function. proteins characterized solution...
Very high quality crystals of tetragonal hen egg-white lysozyme were grown in the Advanced Protein Crystallization Facility (APCF) on board Space Shuttle using a modified free-interface diffusion (FID) reactor designed ad hoc to have longer path. This design allows performance true counter-diffusion experiments. Crystals obtained under classical chemical conditions defined 50 y ago with NaCl as crystallizing agent and acetate pH 4.5 buffer. Counter-diffusion crystallization `physical'...
Microfluidic technology has opened new possibilities for the crystallization of biological macromolecules during past decade. systems offer numerous advantages over conventional crystal growth methods. They enable easy handling nanovolumes solutions, extreme miniaturization, and parallelization assays, especially high-throughput screening applications. Our goal was to design a versatile, low cost, easy-to-use chip based on counter-diffusion that is compatible with on-chip crystallographic...
As adapter molecules to convert the nucleic acid information into amino sequence, tRNAs play a central role in protein synthesis. To fulfill this function reliable way, exhibit highly conserved structural features common all organisms and cellular compartments active translation. However, mitochondria of metazoans, certain dramatic deviations from consensus tRNA structure are described, where some lack D- or T-arm without losing their function. In Enoplea, miniaturization comes an extreme,...
Proteins of the BCL7 family (BCL7A, BCL7B, and BCL7C) are among most recently identified subunits mammalian SWI/SNF chromatin remodeler complex absent from unicellular version this complex. Their function in is unknown, very limited structural information available, despite fact that they mutated several cancer types, notably blood malignancies hence medically relevant. Here, using cryo-electron microscopy combination with biophysical biochemical approaches, we show BCL7A forms a stable,...
Studies were conducted to evaluate the response of cattle and deer ferrets which sedated so they behaved like terminally tuberculous animals, compare this with cattle, sheep possums. Six groups two exposed a ferret possum. Both livestock species showed interest in possum by sniffing licking it, but only briefly touched no or extended investigation was observed. The proportion available time spent physical contact 7.7 times as high for ferret, 5.7 high. behavioural three investigated, limited...
This review presents the goals and principles of microfluidic technologies applied to crystallization biological macromolecules. A comparison devices that are available commercially or described in literature summarizes current state-of-the-art microfluidics. novel chip based on counter-diffusion solute molecules playing role agents is described. Inside channels composing mass transport essentially occurs by diffusion. The made a rigid polymer impermeable gases compatible with crystal...
Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists 60 identical coat protein subunits (CP), carries determinants this specificity. Here, we provide novel insight into GFLV nematodes through comparative structural functional analysis two variants. We isolated mutant strain (GFLV-TD)...
Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, microfluidic device described that enables by counter-diffusion and their direct on-chip analysis serial crystallography at room temperature. Nine `non-model' diverse biomacromolecules, including seven soluble proteins, membrane protein an RNA duplex, were crystallized treated with variety standard techniques micro-seeding, crystal soaking ligands detection...
In the mammalian mitochondrial translation apparatus, proteins and their partner RNAs are coded by two genomes. The nuclear-encoded resemble homologs, whereas coming from rapidly evolving genome have lost critical structural information. This raises question of molecular adaptation these to peculiar RNAs. crystal structure homodimeric bacterial-type human aspartyl-tRNA synthetase (DRS) confirmed a 3D architecture close that Escherichia coli DRS. However, enzyme distinguishes an enlarged...
Abstract In plants, the voltage-dependent anion-selective channel (VDAC) is a major component of pathway involved in transfer RNA (tRNA) translocation through mitochondrial outer membrane. However, way which VDAC proteins interact with tRNAs still unknown. Potato mitochondria contain two proteins, VDAC34 and VDAC36. These composed N-terminal α-helix 19 β-strands forming β-barrel structure, share 75% sequence identity. Here, using both northwestern gel shift experiments, we report that these...
Abstract Mutations in human mitochondrial aminoacyl-tRNA synthetases are associated with a variety of neurodegenerative disorders. The effects these mutations on the structure and function enzymes remain to be established. Here, we investigate six mutants aspartyl-tRNA synthetase correlated leukoencephalopathies. Our integrated strategy, combining an ensemble biochemical biophysical approaches, reveals that diversely affected respect their solubility cellular extracts stability solution, but...