A5082042696- Asthma and respiratory diseases
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Peptidase Inhibition and Analysis
- Genomics, phytochemicals, and oxidative stress
- Estrogen and related hormone effects
- Immune Response and Inflammation
- Vitamin K Research Studies
- Fatty Acid Research and Health
- Mast cells and histamine
- Antimicrobial Peptides and Activities
- Pharmacogenetics and Drug Metabolism
- Allergic Rhinitis and Sensitization
- S100 Proteins and Annexins
- Peroxisome Proliferator-Activated Receptors
- Pediatric health and respiratory diseases
- Receptor Mechanisms and Signaling
- Glutathione Transferases and Polymorphisms
- Neuropeptides and Animal Physiology
- Cytokine Signaling Pathways and Interactions
- Chemical Synthesis and Analysis
- Metabolomics and Mass Spectrometry Studies
- Eosinophilic Esophagitis
- Ferrocene Chemistry and Applications
- Respiratory and Cough-Related Research
Karolinska Institutet
2015-2024
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Cornell University
2020
Princeton University
2020
Federation of American Societies for Experimental Biology
2020
Division of Chemistry
2011-2016
Maresins are produced by macrophages from docosahexaenoic acid (DHA) and exert potent proresolving tissue homeostatic actions. Maresin 1 (MaR1; 7R,14S-dihydroxy-docosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid) is the first identified maresin. Here, we investigate formation, stereochemistry, precursor role of 13,14-epoxy-docosahexaenoic acid, an intermediate in MaR1 biosynthesis. The 14-lipoxygenation DHA human macrophage 12-lipoxygenase (hm12-LOX) gave 14-hydro(peroxy)-docosahexaenoic...
Leukotrienes (LT) are a group of proinflammatory lipid mediators that implicated in the pathogenesis and progression atherosclerosis. Here we report mRNA levels for three key proteins LTB4 biosynthesis, namely 5-lipoxygenase (5-LO), 5-LO-activating protein (FLAP), LTA4 hydrolase (LTA4H), significantly increased human atherosclerotic plaque (n = 72) as compared with healthy controls 6). Neither LTC4 synthase nor any LT receptors exhibits levels. Immunohistochemical staining revealed abundant...
Abstract LL-37/hCAP-18 is the only human member of cathelicidin family and plays an important role in killing various pathogens, as well immune modulation. In this study, we investigated effect LL-37 on bacterial phagocytosis by macrophages demonstrate that enhances IgG-opsonized Gram-negative Gram-positive bacteria a dose- time-dependent manner dTHP-1 cells. addition, enhanced nonopsonized Escherichia coli macrophages. Consistently, elevated expression FcγRs but not complement receptors...
Incubation of human endothelial cells with leukotriene A 4 resulted in the formation leukotrienes B , C D and E . Endothelial did not produce after stimulation ionophore A23187 and/or exogenously added arachidonic acid. However, incubation polymorphonuclear leukocytes together led to an increased synthesis cysteinyl‐containing (364%, mean, n = 11) (52%) as compared alone. Thus, major part recovered mixed cultures was attributable presence cells. Similar incubations fibroblasts or smooth...
Abstract Bioactive peptide LL-37/hCAP18, the only human member of cathelicidin family, plays important roles in killing various pathogens, as well immune modulation. We demonstrate that LL-37 is internalized by macrophages a time-, dose-, temperature-, and sequence–dependent endocytotic process. Both clathrin- caveolae/lipid raft–mediated endocytosis pathways are involved internalization. find P2X7 receptor (P2X7R) an role internalization because significantly less was detected pretreated...
In humans, the antimicrobial peptide LL-37 and leukotriene B4 (LTB4) are important proinflammatory mediators, whereas lipoxin A4 (LXA4) resolvin E1 (RvE1) possess anti-inflammatory, prore-solving properties. Previously, we reported that LTB4 triggers release from human neutrophils (PMNs) and, conversely, promotes production these cells. Here show this effect of is mediated via GPCR FPR2/ALX. (5–30 µg/ml) induces intracellular calcium mobilization in a dose-dependent manner, signal...
Resolution of acute inflammation is an active process coordinated by proresolving lipid mediators (SPMs) such as lipoxins (LXs) and resolvins (Rvs), which are formed the concerted action 2 lipoxygenases (LOs). Because exact molecular mechanisms SPM biosynthesis not completely understood, we aimed to investigate LX D-type Rv formation in human leukocytes HEK293T cells overexpressing leukotriene (LT) pathway enzymes. Activity assays precursor (15-hydroxyeicosatetraenoic acids,...
A cDNA encoding an arachidonate 12‐lipoxygenase from rat brain was obtained by polymerase chain reaction cloning. Primers specific for porcine leukocyte were used to isolate the initial polymerase‐chain‐reaction product (395 bp). The final sequence of coding region (1989 bp) verified analysis several separate products. open reading frame corresponded a protein 662 amino acid residues, with calculated molecular mass 75 305 Da. Also contained six conserved histidines, characteristic all cloned...
Three mutants of recombinant mouse leukotriene A4 (LTA4) hydrolase (3.3.2.6) were produced by site-directed mutagenesis on cDNA. The codons corresponding to His-295, His-299, or Glu-318 replaced encoding tyrosine, and glutamine, respectively. mutated cDNAs expressed in Escherichia coli, the three proteins purified apparent homogeneity. None these contained significant amounts zinc, as determined atomic absorption spectrometry, all them practically devoid both LTA4 peptidase enzyme...