A5018505044- Neuropeptides and Animal Physiology
- Gastroesophageal reflux and treatments
- Neuroscience of respiration and sleep
- Gastrointestinal motility and disorders
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Glaucoma and retinal disorders
- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Dysphagia Assessment and Management
- Biomedical and Chemical Research
- Vagus Nerve Stimulation Research
- Retinal Development and Disorders
- Diet and metabolism studies
- Heart Rate Variability and Autonomic Control
- Orthopedic Surgery and Rehabilitation
- Migraine and Headache Studies
- Nanoparticle-Based Drug Delivery
- Congenital gastrointestinal and neural anomalies
- Nerve injury and regeneration
- Adipose Tissue and Metabolism
- Helicobacter pylori-related gastroenterology studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015-2024
Universitätsklinikum Erlangen
1997-2015
Medizinische Hochschule Hannover
2013
Carol Davila University of Medicine and Pharmacy
2013
Altria (United States)
2013
Universität Ulm
2013
Universität Hamburg
2005
University Medical Center Hamburg-Eppendorf
2005
University of Oslo
2005
University of Edinburgh
2005
Cardiac tissue engineering is an emerging field. The suitability of engineered heart (EHT) for both in vitro and vivo applications will depend on the degree syncytoid formation cardiac myocyte differentiation vitro, contractile function, electrophysiological properties. Here, we demonstrate that myocytes from neonatal rats, when mixed with collagen I matrix factors, cast circular molds, subjected to phasic mechanical stretch, reconstitute ring-shaped EHTs display important hallmarks...
To investigate the fate of apoptotic cells in germinal centers (GCs) patients with systemic lupus erythematosus (SLE).Lymph node biopsy specimens obtained from 7 SLE benign follicular hyperplasia, 5 non-SLE hyperplasia (non-SLE), malignant lymphoma, and 3 dermatopathic lymphadenitis were stained monoclonal antibodies against macrophages (CD68) dendritic (CR2/CD21). TUNEL staining transmission electron microscopy performed to detect cells. Confocal was used evaluate vivo capacity tingible...
Abstract Calcitonin gene‐related peptide (CGRP) is a key mediator in primary headaches including migraine. Animal models of meningeal nociception demonstrate both peripheral and central CGRP effects; however, the target structures remain unclear. To study distribution receptors rat trigeminovascular system we used antibodies recognizing two components receptor, calcitonin receptor‐like receptor (CLR) activity‐modifying protein 1 (RAMP1). In cranial dura mater, CLR RAMP1 immunoreactivity...
Cell grafting has emerged as a novel approach to treat heart diseases refractory conventional therapy. We hypothesize that survival and functional electrical integration of grafts may be improved by engineering cardiac tissue constructs in vitro before grafting.Engineered (EHT) was reconstituted mixing myocytes from neonatal Fischer 344 rats with liquid collagen type I, matrigel, serum-containing culture medium. EHTs were designed circular shape (inner/outer diameter: 8/10 mm; thickness: 1...
Abstract The projections of primary afferents from rostral cervical segments to the brainstem and spinal cord rat were investigated by using anterograde transganglionic transport techniques. Projections whole ganglia compared with those single nerves carrying only exteroceptive or proprioceptive fibers. Injections horseradish peroxidase (HRP) wheat germ agglutinin‐horseradish conjugate (WGA‐HRP) performed into dorsal root C2, C3, C4. Free HRP was applied cut rami C2 greater occipital nerve,...
Inflammatory mediators not only activate "pain-"sensing neurons, the nociceptors, to trigger acute pain sensations, more important, they increase nociceptor responsiveness produce inflammatory hyperalgesia. For example, prostaglandins G<sub>s</sub>-protein-coupled receptors and initiate cAMP- protein kinase A (PKA)-mediated processes. We demonstrate for first time at cellular level that heat-activated ionic currents were potentiated after exposure cAMP activator forskolin in rat nociceptive...
Background— Abnormally elevated blood pressure is the most prevalent risk factor for cardiovascular disease. The large-conductance, voltage- and Ca 2+ -dependent K + (BK) channel has been proposed as an important effector in control of vascular tone by linking membrane depolarization local increases cytosolic to hyperpolarizing outward currents. However, BK may also affect regulating salt fluid homeostasis, particularly adjusting renin-angiotensin-aldosterone system. Methods Results— Here we...
The hippocampal dentate gyrus maintains its capacity to generate new neurons throughout life. In animal models, neurogenesis is increased by cognitive tasks, and experimental ablation of disrupts specific modalities learning memory. humans, the impact on cognition remains unclear. Here, we assessed neurogenic potential in human isolating adult neural stem cells from 23 surgical en bloc hippocampus resections. After proliferation progenitor cell pool vitro identified two distinct patterns....
Headaches can be evoked by activation of meningeal nociceptors, but an involvement pericranial tissues is debated. We aimed to examine a possible extracranial innervation afferents in the rat. For vivo neuronal tracing, dextran amines were applied periosteum underlying temporal muscle. Labeling was observed 2 days later parietal dura mater, trigeminal ganglion, and spinal nucleus with confocal electron microscopy. In hemisected rat head, extracellular recordings made from nerve fibers....
To reinvestigate the innervation pattern of dura mater rat and human middle cranial fossa, morpho-functional substrate headache generation, adjacent extracranial tissues with neuronal in vitro tracing.This study was initiated by recent structural functional findings meningeal afferent fibers which innervate may project to tissues.Anterograde retrograde tracing made formaldehyde fixed hemisected skulls. The fluorescent tracer DiI applied proximally cut nerves distal branches spinosus nerve...
Superparamagnetic iron oxide nanoparticles (SPIONs) with a mixed phase composition (γ-Fe2O3)(1-x)(Fe3O4)x and sizes between 9 20 nm were synthesized via coprecipitation either left uncoated or subsequently surface-stabilized citrate malate anions. The sizes, morphology, surface chemistry, magnetic properties of the characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, superconducting quantum interference device measurements, respectively....
In the mammalian CNS, N -methyl-D-aspartate (NMDA) receptors serve prominent roles in many physiological and pathophysiological processes including pain transmission. For full activation, NMDA require binding of glycine. It is not known whether brain uses changes extracellular glycine to modulate synaptic responses. Here, we show that synaptically released facilitates receptor currents superficial dorsal horn, an area critically involved processing. During high presynaptic activity, from...