A5036894524- Cancer Research and Treatments
- Medical Imaging Techniques and Applications
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Mesenchymal stem cell research
- Radiopharmaceutical Chemistry and Applications
- Glioma Diagnosis and Treatment
- Cell death mechanisms and regulation
- Neuroblastoma Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Cytokine Signaling Pathways and Interactions
- Cancer Mechanisms and Therapy
- Quantum, superfluid, helium dynamics
- interferon and immune responses
- Diet and metabolism studies
- Monoclonal and Polyclonal Antibodies Research
- Particle Detector Development and Performance
- Lung Cancer Research Studies
- Radiation Detection and Scintillator Technologies
- Cancer Immunotherapy and Biomarkers
- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- Peptidase Inhibition and Analysis
University of Tübingen
2016-2024
Siemens (Germany)
2014-2024
STZ eyetrial
2014-2024
Imaging Center
2019
Stem cells` (SC) functional heterogeneity and its poorly understood aetiology impedes clinical development of cell-based therapies in regenerative medicine oncology. Recent studies suggest a strong correlation between the SC migration potential their therapeutic efficacy humans. Designating as denominator heterogeneity, we sought to identify highly migrating subpopulations within different classes evaluate properties comparison parental non-selected cells.We selected from mesenchymal neural...
Background: Antibodies targeting surface expressed disialoganglioside GD2 are increasingly used in neuroblastoma immunotherapy and might also have potential for use radioimmunotherapy.As such targeted treatments benefit from a dedicated theranostic approach, we studied the influence of radiolabeling on binding characteristics ch14.18 antibodies produced by Chinese hamster ovary (CHO) cells evaluated GD2-ImmunoPET as tool therapy planning.Methods: 64 Cu was to reduce radiation burden, which...
This study aims at evaluating the combination of tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL)-receptor 2 (TRAIL-R2)-specific antibody Drozitumab and Smac mimetic BV6 in preclinical glioblastoma models. To this end, effect and/or on apoptosis induction signaling pathways was analyzed cell lines, primary cultures stem-like cells. Here, we report that synergistically induce reduce colony formation several lines (combination index<0.1). Also, profoundly enhances...
BackgroundLimited knowledge of stem cell therapies` mechanisms action hampers their sustainable implementation into the clinic. Specifically, interactions transplanted cells with host vasculature and its implications for therapeutic efficacy are not elucidated. We tested whether adhesion receptors chemokine on can be functionally modulated, consequently if such modulation may substantially affect therapeutically relevant endothelium.MethodsWe investigated effects cationic molecule...
Phenotypic heterogeneity is commonly observed in diseased tissue, specifically tumors. Multimodal imaging technologies can reveal tissue noninvasively vivo, enabling imaging-based profiling of receptors, metabolism, morphology, or function on a macroscopic scale. In contrast, vitro multiomics, immunohistochemistry, histology techniques accurately characterize these heterogeneities the cellular and subcellular scales more comprehensive but ex vivo manner. The complementary information would...
Abstract Identification of prognostic or predictive molecular markers in glioblastoma resection specimens may lead to strategies for therapy stratification and personalized treatment planning. Here, we analyzed primary stem cell (pGSC) cultures the mRNA abundances seven (MSI1, Notch1, nestin, Sox2, Oct4, FABP7 ALDH1A3), three radioresistance invasion (CXCR4, IK Ca BK ). From these abundances, an signature was deduced which describes mesenchymal‐to‐proneural expression profile individual GSC...
Colorectal cancer is one of the most common forms cancer, and development novel tools for detection efficient treatment metastases needed. One promising approach use radiolabeled antibodies positron emission tomography (PET) imaging radioimmunotherapy. Since carcinoembryonic antigen (CEA) an important target in colorectal CEA-specific M5A antibody has been extensively studied subcutaneous xenograft models; however, not yet tested advanced models liver metastases. The aim this study was to...
Abstract Senescence influences treatment outcome in cancer. Therefore it is of great interest to develop a diagnostic tool detect senescent cells cancer patients. Here we describe the evaluation PET tracer FPyGal for non-invasive imaging ß-galactosidase as surrogate marker senescence. In vitro, induced senescence HCT116 by doxorubicin, liver progenitor cell line p53-reactivation and two carcinoma lines ribosomal checkpoint inhibitor (RCI). The were then incubated with uptake was determined...
Tumor hypoxia and other microenvironmental factors are key determinants of treatment resistance. Hypoxia positron emission tomography (PET) functional magnetic resonance imaging (MRI) established prognostic modalities to identify radiation resistance in head-and-neck cancer (HNC). The aim this preclinical study was develop a multi-parametric parameter specifically for focal radiotherapy (RT) dose escalation using HNC xenografts different sensitivities.A total eight human xenograft models...
Abstract Prognosis of glioblastoma patients is still poor despite multimodal therapy. The highly brain‐infiltrating growth in concert with a pronounced therapy resistance particularly mesenchymal stem‐like cells (GSCs) has been proposed to contribute failure. Recently, we have shown that mesenchymal‐to‐proneural mRNA signature patient derived GSC‐enriched (pGSC) cultures associates vitro radioresistance and gel invasion. Importantly, this pGSC prognostic for patients' tumor recurrence...
Radiosynthesis of [1-11C]acetate is well described in literature, but all syntheses either require adaptations complex commercial synthesizers or rely on closed-source hardware and software control. Arduino microcontrollers are ideal for the compact, flexible, inexpensive control low-complexity hardware, making them particularly suited radiochemistry where operation space-limited shielded hot cells mandatory. We established a radiosynthesis module combination with [11C]MeI available almost...
Senescence, a durable cell cycle arrest, is stress response influencing the treatment outcome in cancer. Currently senescence-associated ß-galactosidase gold standard biomarker for ex vivo senescence detection. Here we developed PET tracer [18F] FPyGal detection of senescent cells cancer patients.
Abstract The inner clock of biological organisms plays a pivotal role and has strong effects on metabolic processes such as glucose consumption. Since the commonly used positron emission tomography (PET) tracer 18 F-flourodeoxygucose (FDG) is analogue, it not surprising that FDG distribution in mice humans been shown to succumb daily rhythms. In preclinical studies, circadian rhythm animals often considered, studies are performed at different times day. Only few have analyzed effect uptake...
Abstract Senescence is a response to cellular stress and induces durable cell cycle arrest, which has essential effects on metabolism as well treatment outcome in cancer. Therefore the development of diagnostic tool detect senescent cells vivo cancer patients great interest. Here, novel PET tracer for non-invasive imaging beta-galactosidase surrogate marker senescence described. Three different vitro models were used test tracer. In HCT116 was induced by doxorubicin, while an HRas driven...
Senescence influences treatment outcome in cancer. Therefore it is of great interest to develop a diagnostic tool detect senescent cells cancer patients. Here we describe the evaluation PET tracer FPyGal for non-invasive imaging ß-galactosidase as surrogate marker senescence. In vitro, induced senescence HCT116 by doxorubicin, liver progenitor cell line p53-reactivation and two carcinoma lines ribosomal checkpoint inhibitor (RCI). The were then incubated with uptake was determined...