A5071792075- Glioma Diagnosis and Treatment
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Cancer, Stress, Anesthesia, and Immune Response
- Radiomics and Machine Learning in Medical Imaging
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Ion Transport and Channel Regulation
- Medical Imaging Techniques and Applications
- Biomedical and Engineering Education
- Nanoplatforms for cancer theranostics
- Pharmacological Effects and Toxicity Studies
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- T-cell and B-cell Immunology
- Advanced Radiotherapy Techniques
- Cardiac electrophysiology and arrhythmias
- Electrochemical Analysis and Applications
- Chemical Reactions and Isotopes
University of Tübingen
2019-2024
University Children's Hospital Tübingen
2020-2024
Tumor treating fields (TTFields) represent a novel FDA-approved treatment modality for patients with newly diagnosed or recurrent glioblastoma multiforme. This therapy applies intermediate frequency alternating electric low intensity to the tumor volume by use of non-invasive transducer electrode arrays. Mechanistically, TTFields have been proposed impair formation mitotic spindle apparatus and cytokinesis. In order identify further potential molecular targets, here effects on...
Abstract Background The IDH-wildtype glioblastoma (GBM) patients have a devastating prognosis. Here, we analyzed the potential prognostic value of global DNA methylation tumors. Methods 492 primary samples and 31 relapsed samples, each treated with combination therapy, 148 radiation alone were compared patient survival. We determined mean values estimated immune cell infiltration from data. Moreover, 23 GBM lines was profiled correlated to their cellular radiosensitivity as measured by...
The intermediate-conductance calcium-activated potassium channel KCa3.1 has been proposed to be a new potential target for glioblastoma treatment. This study analyzed the effect of combined irradiation and KCa3.1-targeting with TRAM-34 in syngeneic, immune-competent orthotopic SMA-560/VM/Dk glioma mouse model. Whereas neither nor treatment alone meaningfully prolonged survival animals, combination significantly mice. We found an irradiation-induced hyperinvasion cells into brain, which was...
Abstract Identification of prognostic or predictive molecular markers in glioblastoma resection specimens may lead to strategies for therapy stratification and personalized treatment planning. Here, we analyzed primary stem cell (pGSC) cultures the mRNA abundances seven (MSI1, Notch1, nestin, Sox2, Oct4, FABP7 ALDH1A3), three radioresistance invasion (CXCR4, IK Ca BK ). From these abundances, an signature was deduced which describes mesenchymal‐to‐proneural expression profile individual GSC...
Mesenchymal glioblastoma stem cells (GSCs), a subpopulation in that are responsible for therapy resistance and tumor spreading the brain, reportedly upregulate aldehyde dehydrogenase isoform-1A3 (ALDH1A3) which can be inhibited by disulfiram (DSF), an FDA-approved drug formerly prescribed alcohol use disorder. Reportedly, DSF combination with Cu2+ ions exerts multiple tumoricidal, chemo- radio-therapy-sensitizing effects several entities. The present study aimed to quantify these vitro,...
Abstract Prognosis of glioblastoma patients is still poor despite multimodal therapy. The highly brain‐infiltrating growth in concert with a pronounced therapy resistance particularly mesenchymal stem‐like cells (GSCs) has been proposed to contribute failure. Recently, we have shown that mesenchymal‐to‐proneural mRNA signature patient derived GSC‐enriched (pGSC) cultures associates vitro radioresistance and gel invasion. Importantly, this pGSC prognostic for patients' tumor recurrence...
Many tumor cells express highly elevated activities of voltage-gated K+ channels in the plasma membrane which are indispensable for growth. To test channel function during DNA damage response, we subjected human chronic myeloid (CML) leukemia to sub-lethal doses ionizing radiation (0, 1 or 5 Gy, 6 MV photons) and determined mRNA protein abundance, activity, K+-channel dependent Ca2+ signaling, cell cycle progression, clonogenic survival by RT-PCR, Western Blotting, whole-cell patch clamp...
Introduction Hypoxia can drive tumor progression, suppress anti-tumor immunity, and reduce the effectiveness of radiotherapy chemotherapy. This study aimed to assess impact remote ischemic conditioning (RIC) on oxygenation (sO2) immune response. Material methods Fourteen B16-Ova tumor-bearing C57BL/6N mice received six 5-minute RIC cycles, while another fourteen underwent anesthesia only. Pimonidazole was administered 1.5 hours before sacrifice. Blood flow, sO2, hemoglobin levels were...
Reportedly, the intermediate-conductance Ca2+-activated potassium channel KCa3.1 contributes to invasion of glioma cells into healthy brain tissue and resistance temozolomide ionizing radiation. Therefore, has been proposed as a potential target in therapy. The aim present study was assess variability temozolomide- radiation-sensitizing effects conferred by blocking agent TRAM-34 between five different cell lines grown differentiated bulk tumor or under stem cell-enriching conditions. As...
Abstract Background Recently, a molecular signature describing the mesenchymal / proneural features of primary stem-cell-enriched glioblastoma cultures was identified correlating with invasiveness, radiation sensitivity and patient-outcome. However, generating characterizing is time-consuming this might hamper translation into clinical concepts. The aim study to evaluate use standard preoperative MR imaging (T1ce T2FLAIR sequences) predict thus enable development treatment concepts based on...