A5069825430- Ion channel regulation and function
- Glioma Diagnosis and Treatment
- Drug Solubulity and Delivery Systems
- Crystallization and Solubility Studies
- Nicotinic Acetylcholine Receptors Study
- Cardiac electrophysiology and arrhythmias
- Genetic Neurodegenerative Diseases
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Analytical Methods in Pharmaceuticals
- Histone Deacetylase Inhibitors Research
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Pharmacological Effects and Toxicity Studies
- Neuroscience and Neural Engineering
- Heat shock proteins research
- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Microencapsulation and Drying Processes
- Autophagy in Disease and Therapy
- Ion Transport and Channel Regulation
- Plant-based Medicinal Research
- Protein purification and stability
- Nanoparticles: synthesis and applications
University of Tübingen
2014-2023
University Children's Hospital Tübingen
2020-2021
Goethe University Frankfurt
2017-2020
Institute of Molecular Biology
2020
Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology
2015-2018
University of Alberta
2017
Dissolution testing with biorelevant media has become widespread in the pharmaceutical industry as a means of better understanding how drugs and formulations behave gastrointestinal tract. Until now, however, there have been few attempts to gauge reproducibility results obtained these methods. The aim this study was determine interlaboratory dissolution testing, using paddle apparatus (USP 2). Thirteen industrial three academic laboratories participated study. All were provided standard...
Tumor treating fields (TTFields) represent a novel FDA-approved treatment modality for patients with newly diagnosed or recurrent glioblastoma multiforme. This therapy applies intermediate frequency alternating electric low intensity to the tumor volume by use of non-invasive transducer electrode arrays. Mechanistically, TTFields have been proposed impair formation mitotic spindle apparatus and cytokinesis. In order identify further potential molecular targets, here effects on...
Infiltration of the brain by glioblastoma cells reportedly requires Ca2+ signals and BK K+ channels that program drive cell migration, respectively. Ionizing radiation (IR) has been shown to induce expression chemokine SDF-1, alter signaling, stimulate migration cells. Here, we quantified fractionated IR-induced migration/brain infiltration human in vitro an orthotopic mouse model analyzed role SDF-1/CXCR4 signaling channels. To this end, radiation-induced migratory phenotypes T98G far-red...
Abstract Ca2+-activated K+ channels, such as BK and IK have been proposed to fulfill pivotal functions in neoplastic transformation, malignant progression, brain infiltration of glioblastoma cells. Here, the ionizing radiation (IR) effect channel targeting was tested human channels were inhibited pharmacologically by TRAM-34 or genetically knockdown, cells irradiated with 6 MV photons activity, Ca2+ signaling, cell cycling, residual double-strand breaks, clonogenic survival determined. In...
TRPM8 is a Ca2+-permeable nonselective cation channel belonging to the melastatin sub-group of transient receptor potential (TRP) family. aberrantly overexpressed in variety tumor entities including glioblastoma multiforme where it reportedly contributes invasion. The present study aimed disclose further functions glioma biology particular upon cell injury by ionizing radiation. To this end, TCGA data base was queried expose mRNA abundance human specimens and immunoblotting performed analyze...
Abstract: Drug product performance testing is an important part of quality-by-design approaches, but this process often lacks the underlying mechanistic understanding complex interactions between disintegration and dissolution processes involved. Whereas a recent draft guideline by US Food Administration (FDA) has allowed replacement with testing, mentioned criteria are not globally accepted. This study provides scientific justification for using rather than as quality control method certain...
Background/Aims: Valproic acid (VPA), an anticonvulsant and mood-stabilizing drug is used to treat epileptic seizure of glioblastoma patients. Besides its antiepileptic activity, VPA has been attributed further functions that improve the clinical outcome Those comprise inhibition some histone deacetylase (HDAC) isoforms which reportedly may result in radiosensitization. Retrospective analysis patient data, however, could not unequivocally confirm a prolonged survival patients receiving VPA....
Abstract Background The IDH-wildtype glioblastoma (GBM) patients have a devastating prognosis. Here, we analyzed the potential prognostic value of global DNA methylation tumors. Methods 492 primary samples and 31 relapsed samples, each treated with combination therapy, 148 radiation alone were compared patient survival. We determined mean values estimated immune cell infiltration from data. Moreover, 23 GBM lines was profiled correlated to their cellular radiosensitivity as measured by...
Abstract Identification of prognostic or predictive molecular markers in glioblastoma resection specimens may lead to strategies for therapy stratification and personalized treatment planning. Here, we analyzed primary stem cell (pGSC) cultures the mRNA abundances seven (MSI1, Notch1, nestin, Sox2, Oct4, FABP7 ALDH1A3), three radioresistance invasion (CXCR4, IK Ca BK ). From these abundances, an signature was deduced which describes mesenchymal‐to‐proneural expression profile individual GSC...
The physical and chemical stability of various biorelevant media (FaSSGF, FaSSIF V1, V2, V3, FeSSIF) were investigated over periods after preparation up to 120 h at room temperature (RT) (22 °C) 37 °C.It was shown that instant powders are not a source microbiological contamination.It also established the characteristics FaSSGF FeSSIF invariant with time, so they can be used immediately preparation, i.e., without any equilibration time.Alternatively, stored for 96 RT before use.By contrast,...
Mesenchymal glioblastoma stem cells (GSCs), a subpopulation in that are responsible for therapy resistance and tumor spreading the brain, reportedly upregulate aldehyde dehydrogenase isoform-1A3 (ALDH1A3) which can be inhibited by disulfiram (DSF), an FDA-approved drug formerly prescribed alcohol use disorder. Reportedly, DSF combination with Cu2+ ions exerts multiple tumoricidal, chemo- radio-therapy-sensitizing effects several entities. The present study aimed to quantify these vitro,...
Glioblastoma (GBM) is a highly aggressive brain tumor. Resistance mechanisms in GBM present an array of challenges to understand its biology and develop novel therapeutic strategies. We investigated the role TSG, MTUS1/ATIP1 glioma. Glioma specimen, cells low passage sphere cultures (GSC) were analyzed for expression at RNA protein level. Methylation analyses done by bisulfite sequencing (BSS). The consequence chemotherapy irradiation on ATIP1 influence different cellular levels survival was...
Many tumor cells express highly elevated activities of voltage-gated K+ channels in the plasma membrane which are indispensable for growth. To test channel function during DNA damage response, we subjected human chronic myeloid (CML) leukemia to sub-lethal doses ionizing radiation (0, 1 or 5 Gy, 6 MV photons) and determined mRNA protein abundance, activity, K+-channel dependent Ca2+ signaling, cell cycle progression, clonogenic survival by RT-PCR, Western Blotting, whole-cell patch clamp...