A5026220114- Neurogenesis and neuroplasticity mechanisms
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Natural Language Processing Techniques
- Axon Guidance and Neuronal Signaling
- Cancer Cells and Metastasis
- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Logic, programming, and type systems
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Model-Driven Software Engineering Techniques
- Vitamin D Research Studies
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Nerve injury and regeneration
- RNA Research and Splicing
- Logic, Reasoning, and Knowledge
- interferon and immune responses
- Mesenchymal stem cell research
Goethe University Frankfurt
2013-2024
Hirosaki University
2024
University Hospital Frankfurt
2006-2023
German Cancer Research Center
2013-2018
Heidelberg University
2013-2018
Frankfurt Cancer Institute
2018
Deutschen Konsortium für Translationale Krebsforschung
2015
Vita-Salute San Raffaele University
2010
Leiden University Medical Center
2010
Stefan University
2009
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
New neurons are generated from stem cells in a few regions of the adult mammalian brain. Here we provide evidence for generation dopaminergic projection type that lost Parkinson's disease rodent brain and show rate neurogenesis is increased after lesion. The number new under physiological conditions substantia nigra pars compacta was found to be several orders magnitude smaller than granular cell layer dentate gyrus hippocampus. However, if neuronal turnover constant, entire population could...
Mechanisms behind how the immune system signals to brain in response systemic inflammation are not fully understood. Transgenic mice expressing Cre recombinase specifically hematopoietic lineage a reporter background display recombination and marker gene expression Purkinje neurons. Here we show that reportergene neurons is caused by intercellular transfer of functional messenger RNA from cells into absence cell fusion. In vitro purified secreted extracellular vesicles (EVs) blood contain...
Extracellular vesicles (EVs) have been shown to transfer various molecules, including functional RNA between cells and this process has suggested be particularly relevant in tumor-host interactions. However, data on EV-mediated obtained primarily by vitro experiments or involving ex vivo manipulations likely affecting its biology, leaving their physiological relevance unclear. We engineered glioma carcinoma tumor express Cre recombinase showing release of EVs containing mRNA EV subfractions...
Abstract The intestinal microbiota influences mammalian host physiology in health and disease locally the gut but also organs devoid of direct contact with bacteria such as liver brain. Extracellular vesicles (EVs) or outer membrane (OMVs) released by microbes are increasingly recognized for their potential role biological shuttle systems inter‐kingdom communication. However, physiologically relevant evidence transfer functional biomolecules from to individual cells OMVs vivo is scarce. By...
The adult human brain retains the capacity to generate new neurons in hippocampal formation (Eriksson et al., 1998) and neuronal progenitor cells (NPCs) forebrain (Bernier 2000), but what extent it is capable of reacting injuries, such as ischemia, not known. We analyzed postmortem tissue from normal pathological ( n = 54) study cellular response ischemic injury forebrain. observed that expressing NPC marker polysialylated neural adhesion cell molecule (PSA-NCAM) are continuously generated...
The number of cells in an organ is regulated by mitogens and trophic factors that impinge on intrinsic determinants proliferation apoptosis. We here report the identification additional mechanism to control cell brain: EphA7 induces ephrin-A2 reverse signaling, which negatively regulates neural progenitor proliferation. Cells stem niche adult brain proliferate more have a shorter cycle mice lacking ephrin-A2. increased accompanied higher olfactory bulb. Disrupting interaction between...
Glioblastoma cells with stem-like properties control brain tumour growth and recurrence. Here, we show that endogenous neural precursor perform an anti-tumour response by specifically targeting cells. In vitro, predominantly express bone morphogenetic protein-7; protein-7 is constitutively released from neurospheres induces canonical protein signalling in glioblastoma Exposure of human murine to neurosphere-derived stem cell differentiation, attenuates marker expression reduces self-renewal...
Abstract In various types of cancers including glioblastoma, accumulating evidence show the existence cancer stem‐like cells ( CSCs ), characterized by stem cell marker expression, capability differentiation and self‐renewal, high potential for tumor propagation in vivo . LGR5 , whose expression is positively regulated Wnt signaling pathway, a intestinal mucosa hair follicle skin. As also involved brain development, function maintenance to be assessed. Our study showed that transcript level...
ABSTRACT Vesicular stomatitis virus (VSV)-based oncolytic virotherapy has the potential to significantly improve prognosis of aggressive malignancies such as brain cancer. However, VSV's inherent neurotoxicity hindered clinical development so far. Given that this neurotropism is attributed glycoprotein VSV-G, VSV was pseudotyped with nonneurotropic envelope lymphocytic choriomeningitis (LCMV-GP→VSV-GP). Compared VSV, VSV-GP showed enhanced infectivity for cancer cells in vitro while sparing...
Communication with the hematopoietic system is a vital component of regulating brain function in health and disease. Traditionally, major routes considered for this neuroimmune communication are by individual molecules such as cytokines carried blood, neural transmission, or, more severe pathologies, entry peripheral immune cells into brain. In addition, functional mRNA from blood can be directly transferred to neurons via extracellular vesicles (EVs), but parameters that determine their...
Transplanted hematopoietic cells have previously been shown to contribute of other tissues by cell fusion. We wanted elucidate whether this phenomenon fusion also occurs under physiological conditions. Using a transgenic mouse reporter system irreversibly label the lineage, we were able test their contribution in absence any additional and potentially confounding factors such as irradiation or chemoablation. found genetically marked, fused Purkinje neurons well hepatocytes numbers comparable...
Complementation of pluripotency genes may improve adult stem cell functions.Here we show that clonally expandable, telomerase expressing progenitor cells can be isolated from peripheral blood children. The surface marker profile the expanded is distinct hematopoietic or mesenchymal stromal cells, and resembles embryonic multipotent mesoangioblasts. Cell numbers proliferative capacity correlated with donor age. Isolated circulating mesoangioblasts (cMABs) express markers Klf4, c-Myc, as well...
This study aims at evaluating the combination of tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL)-receptor 2 (TRAIL-R2)-specific antibody Drozitumab and Smac mimetic BV6 in preclinical glioblastoma models. To this end, effect and/or on apoptosis induction signaling pathways was analyzed cell lines, primary cultures stem-like cells. Here, we report that synergistically induce reduce colony formation several lines (combination index<0.1). Also, profoundly enhances...
The progression of human degenerative and hypoxic/ischemic diseases is accompanied by widespread cell death. One death process linking iron-catalyzed reactive species with lipid peroxidation ferroptosis, which shows hallmarks both programmed necrotic in vitro. While evidence ferroptosis neurodegenerative disease indicated iron accumulation involvement lipids, a stable marker for has not been identified. Its prevalence thus undetermined pathophysiology, impeding recognition areas clinical...