A5029161591- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Neurogenesis and neuroplasticity mechanisms
- Forensic Anthropology and Bioarchaeology Studies
- Epigenetics and DNA Methylation
- Retinal Development and Disorders
- Cardiovascular Disease and Adiposity
- Archaeology and ancient environmental studies
- Paleopathology and ancient diseases
- Forensic and Genetic Research
- Nerve injury and regeneration
- Cancer Genomics and Diagnostics
- Glaucoma and retinal disorders
- Telomeres, Telomerase, and Senescence
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Pancreatic and Hepatic Oncology Research
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- Prenatal Screening and Diagnostics
- Axon Guidance and Neuronal Signaling
- Cancer-related gene regulation
- Zebrafish Biomedical Research Applications
- Neuroscience and Neuropharmacology Research
Karolinska Institutet
2015-2024
Stockholm University
2008-2023
Integrated Cardio Metabolic Centre
2018-2021
AstraZeneca (Sweden)
2018-2021
Karolinska University Hospital
2008-2020
Lawrence Livermore National Laboratory
2008-2009
Institute of Molecular and Cell Biology
2009
Uppsala University
2009
Charité - Universitätsmedizin Berlin
2008
Humboldt-Universität zu Berlin
2008
Abstract Methylation patterns of circulating cell-free DNA (cfDNA) contain rich information about recent cell death events in the body. Here, we present an approach for unbiased determination tissue origins cfDNA, using a reference methylation atlas 25 human tissues and types. The method is validated silico simulations as well vitro mixes from different sources at known proportions. We show that plasma cfDNA healthy donors originates white blood cells (55%), erythrocyte progenitors (30%),...
OBJECTIVE Adipose tissue may contain few large adipocytes (hypertrophy) or many small (hyperplasia). We investigated factors of putative importance for adipose morphology. RESEARCH DESIGN AND METHODS Subcutaneous adipocyte size and total fat mass were compared in 764 subjects with BMI 18–60 kg/m2. A morphology value was defined as the difference between measured volume expected given by a curved-line fit body related to insulin values. In 35 subjects, vivo turnover exploiting incorporation...
Significance We describe a blood test for detection of cell death in specific tissues based on two principles: ( i ) dying cells release fragmented DNA to the circulation, and ii each type has unique methylation pattern. have identified tissue-specific markers developed method sensitive these plasma or serum. demonstrate utility identification pancreatic β-cell 1 diabetes, oligodendrocyte relapsing multiple sclerosis, brain patients after traumatic ischemic damage, exocrine pancreas cancer...
Stem cells generate neurons in discrete regions the postnatal mammalian brain. However, extent of neurogenesis adult human brain has been difficult to establish. We have taken advantage integration (14)C, generated by nuclear bomb tests during Cold War, DNA establish age major areas cerebral neocortex. Together with analysis neocortex from patients who received BrdU, which integrates dividing cells, our results demonstrate that, whereas nonneuronal turn over, are not adulthood at detectable...
Abstract DNA methylation is a fundamental epigenetic mark that governs gene expression and chromatin organization, thus providing window into cellular identity developmental processes 1 . Current datasets typically include only fraction of sites are often based either on cell lines underwent massive changes in culture or tissues containing unspecified mixtures cells 2–5 Here we describe human methylome atlas, deep whole-genome bisulfite sequencing, allowing fragment-level analysis across...
The number of cells in an organ is regulated by mitogens and trophic factors that impinge on intrinsic determinants proliferation apoptosis. We here report the identification additional mechanism to control cell brain: EphA7 induces ephrin-A2 reverse signaling, which negatively regulates neural progenitor proliferation. Cells stem niche adult brain proliferate more have a shorter cycle mice lacking ephrin-A2. increased accompanied higher olfactory bulb. Disrupting interaction between...
Cancer inflicts damage to surrounding normal tissues, which can culminate in fatal organ failure. Here, we demonstrate that cell death organs affected by cancer be detected tissue-specific methylation patterns of circulating cell-free DNA (cfDNA). We elevated levels hepatocyte-derived cfDNA the plasma patients with liver metastases originating from different primary tumors, compared without metastases. In addition, localized pancreatic or colon showed hepatocyte cfDNA, suggesting inflicted...
Age determination of unknown human bodies is important in the setting a crime investigation or mass disaster because age at death, birth date, and year death as well gender can guide investigators to correct identity among large number possible matches. Traditional morphological methods used by anthropologists determine are often imprecise, whereas chemical analysis tooth dentin, such aspartic acid racemization, has shown reproducible more precise results. In this study, we analyzed teeth...
In most countries today, obesity is increasing at an almost epidemic rate and creating a considerable public health problem by elevating the risk of cardiovascular disease metabolic disorders such as type 2 diabetes. It possible that, result, life expectancy will begin to decline in developed countries; this would be first occurrence recent history. Factors that influence fat mass adulthood remain incompletely understood, but increased lipid storage predeveloped cells, or adipocytes,...
Abstract Differences in white adipose tissue (WAT) lipid turnover between the visceral (vWAT) and subcutaneous (sWAT) depots may cause metabolic complications obesity. Here we compare triglyceride age and, thereby, vWAT sWAT biopsies from 346 individuals find that storage capacity are increased overweight or obese individuals. Visceral is only excessively associated with a lower removal capacity. Thus, although higher than vWAT, former plateaus at substantially levels of excess WAT mass...