A5015931743- Pancreatic function and diabetes
- Diabetes and associated disorders
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Diabetes Management and Research
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Metabolism, Diabetes, and Cancer
- SARS-CoV-2 detection and testing
- Congenital heart defects research
- Diet, Metabolism, and Disease
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Pancreatitis Pathology and Treatment
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Genetic Syndromes and Imprinting
- Molecular Biology Techniques and Applications
- COVID-19 epidemiological studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Prenatal Screening and Diagnostics
Hebrew University of Jerusalem
2015-2025
Hadassah Medical Center
2018-2025
Alzheimer's Association of Israel
2024
Weizmann Institute of Science
2024
Center for Neuro-Oncology
2023
Association for Symbolic Logic
2023
iRobot (United States)
2020
Augusta Victoria Hospital
2013
Johns Hopkins University
2013
Cancer Research Center
2013
Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, less hands-on time. We implemented CEL-Seq2 on Fluidigm's C1 system, providing its first single-cell, on-chip barcoding detected gene expression changes accompanying the progression through cell cycle in mouse fibroblast cells. also compare Smart-Seq to demonstrate CEL-Seq2's increased...
Abstract Methylation patterns of circulating cell-free DNA (cfDNA) contain rich information about recent cell death events in the body. Here, we present an approach for unbiased determination tissue origins cfDNA, using a reference methylation atlas 25 human tissues and types. The method is validated silico simulations as well vitro mixes from different sources at known proportions. We show that plasma cfDNA healthy donors originates white blood cells (55%), erythrocyte progenitors (30%),...
The regulated phosphorylation of ribosomal protein (rp) S6 has attracted much attention since its discovery in 1974, yet physiological role remained obscure. To directly address this issue, we have established viable and fertile knock-in mice, whose rpS6 contains alanine substitutions at all five phosphorylatable serine residues (rpS6 P-/- ). Here show that contrary to the widely accepted model, mutation does not affect translational control TOP mRNAs. mouse embryo fibroblasts (MEFs) display...
The mechanisms that regulate pancreatic β cell mass are poorly understood. While autoimmune and pharmacological destruction of insulin-producing cells is often irreversible, adult does fluctuate in response to physiological cues including pregnancy insulin resistance. This plasticity points the possibility harnessing regenerative capacity treat diabetes. We developed a transgenic mouse model study dynamics regeneration from diabetic state. Following doxycycline administration, mice expressed...
Significance We describe a blood test for detection of cell death in specific tissues based on two principles: ( i ) dying cells release fragmented DNA to the circulation, and ii each type has unique methylation pattern. have identified tissue-specific markers developed method sensitive these plasma or serum. demonstrate utility identification pancreatic β-cell 1 diabetes, oligodendrocyte relapsing multiple sclerosis, brain patients after traumatic ischemic damage, exocrine pancreas cancer...
Abstract DNA methylation is a fundamental epigenetic mark that governs gene expression and chromatin organization, thus providing window into cellular identity developmental processes 1 . Current datasets typically include only fraction of sites are often based either on cell lines underwent massive changes in culture or tissues containing unspecified mixtures cells 2–5 Here we describe human methylome atlas, deep whole-genome bisulfite sequencing, allowing fragment-level analysis across...
Senescence is a cellular phenotype present in health and disease, characterized by stable cell-cycle arrest an inflammatory response called senescence-associated secretory (SASP). The SASP important influencing the behavior of neighboring cells altering microenvironment; yet, this role has been mainly attributed to soluble factors. Here, we show that both factors small extracellular vesicles (sEVs) are capable transmitting paracrine senescence nearby cells. Analysis individual internalizing...
Detection of cardiomyocyte death is crucial for the diagnosis and treatment heart disease. Here we use comparative methylome analysis to identify genomic loci that are unmethylated specifically in cardiomyocytes, develop these as biomarkers quantify DNA circulating cell-free (cfDNA) derived from dying cells. Plasma healthy individuals contains essentially no cfDNA, consistent with minimal cardiac turnover. Patients acute ST-elevation myocardial infarction show a robust cfDNA signal...
The Oxford Nanopore (ONT) platform provides portable and rapid genome sequencing, its ability to natively profile DNA methylation without complex sample processing is attractive for point-of-care real-time sequencing. We recently demonstrated ONT shallow whole-genome sequencing detect copy number alterations (CNAs) from the circulating tumor (ctDNA) of cancer patients. Here, we show that cell type cancer-specific changes can also be detected, as well cancer-associated fragmentation...
The risk of early recurrent events after stroke remains high despite currently established secondary prevention strategies1. Risk is particularly in patients with atherosclerosis, more than 10% experiencing events1,2. However, the enormous medical burden this clinical phenomenon, underlying mechanisms leading to increased vascular and are largely unknown. Here, using a novel mouse model stroke-induced ischaemia, we show that leads activation AIM2 inflammasome vulnerable atherosclerotic...