A5043352559- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Pancreatic and Hepatic Oncology Research
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Liver Disease Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Neuroendocrine Tumor Research Advances
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Adenosine and Purinergic Signaling
- RNA regulation and disease
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
VA Tennessee Valley Healthcare System
2016-2025
Vanderbilt University Medical Center
2016-2025
Nashville Oncology Associates
2018-2024
Vanderbilt University
2015-2024
Alzheimer's Association of Israel
2024
The Medical Center of Aurora
2024
Ann Arbor Center for Independent Living
2024
Research Network (United States)
2023
The University of Texas at Austin
2023
Pancreas Centre (Canada)
2022
The recent success of pancreatic islet transplantation has generated considerable enthusiasm. To better understand the quality and characteristics human islets used for transplantation, we performed detailed analysis architecture composition using confocal laser scanning microscopy. Human from six separate isolations provided by three different isolation centers were compared with isolated mouse non-human primate islets. As expected histological sections murine pancreas, in α δ cells resided...
Type 2 diabetes (T2DM) commonly arises from islet β cell failure and insulin resistance. Here, we examined the sensitivity of key islet-enriched transcription factors to oxidative stress, a condition associated with dysfunction in both type 1 (T1DM) T2DM. Hydrogen peroxide treatment lines induced cytoplasmic translocation MAFA NKX6.1. In parallel, ability nuclear PDX1 bind endogenous target gene promoters was also dramatically reduced, whereas activity other transcriptional regulators...
T cells target peptide combos One of the enduring mysteries autoimmunity is identity specific proteins targeted by autoimmune cells. Delong et al. used mass spectrometry to elucidate targets isolated from a mouse model type 1 diabetes. hybrid peptides formed covalent linking derived pro-insulin other found in pancreatic beta islets two individuals with diabetes also recognized such peptides, suggesting that they may play an important role driving disease. Science , this issue p. 711
To investigate molecular mechanisms controlling islet vascularization and revascularization after transplantation, we examined pancreatic expression of three families angiogenic factors their receptors in differentiating endocrine cells adult islets. Using intravital lectin labeling, demonstrated that development microvasculature establishment blood flow occur concomitantly with morphogenesis. Our genetic data indicate vascular endothelial growth factor (VEGF)-A is a major regulator...
Complete lack of transcription factor PDX-1 leads to pancreatic agenesis, whereas heterozygosity for mutations has been recently noted in some individuals with maturity-onset diabetes the young (MODY) and type 2 diabetes. To determine how alterations affect islet function, we examined insulin secretion physiology mice one allele inactivated. PDX-1+/− had a normal fasting blood glucose content but impaired tolerance secreted less during testing. The expression transporter islets from...
OBJECTIVE Conditional gene targeting has been extensively used for in vivo analysis of function β-cell biology. The objective this study was to examine whether mouse transgenic Cre lines, mediate β-cell– or pancreas-specific recombination, also drive expression the brain. RESEARCH DESIGN AND METHODS Transgenic lines driven by Ins1, Ins2, and Pdx1 promoters were bred R26R reporter strains. activity assessed β-galactosidase yellow fluorescent protein pancreas Endogenous monitored using...
OBJECTIVE:This article examines the foundation of β-cell failure in type 2 diabetes (T2D) and suggests areas for future research on underlying mechanisms that may lead to improved prevention treatment.
Many patients with type 1 diabetes (T1D) have residual β cells producing small amounts of C-peptide long after disease onset but develop an inadequate glucagon response to hypoglycemia following T1D diagnosis. The features these and α in the islet endocrine compartment are largely unknown, due difficulty comprehensive investigation. By studying pancreas isolated islets, we show that remnant appeared maintain several aspects regulated insulin secretion. However, function was markedly reduced,...
To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains.Hyperinsulinemic-euglycemic (approximately 8.5 mmol/l) and -hypoglycemic 3.0 clamps were done catheterized, 5-h-fasted mice to assess insulin action hypoglycemic counter-regulatory responsiveness. Hyperglycemic 15 secretion compared with results perifused islets.Insulin secretory phenotypes varied considerably four strains. In vivo was greatest 129X1/Sv mice, but the response hypoglycemia...
<h3>Context</h3> A decline in the number of physician-scientists has been identified United States for at least two decades. Although many mechanisms have proposed to reverse this trend, most these concentrated on MD/PhD programs, research subspecialty fellowships, and other approaches later physician training. Few emphasized early medical student experiences as a contributing solution. <h3>Objective</h3> To determine effect experience career choices attitudes about biomedical research....
Pancreatic islet transplantation is an emerging therapy for type 1 diabetes. To survive and function, transplanted islets must revascularize because isolation severs arterial venous connections; the current paradigm that revascularization originates from transplant recipient. Because isolated retain intraislet endothelial cells, we determined whether these cells contribute to using a murine model with tagged (lacZ knock-in Flk-1/VEGFR2 gene) human islets. At 3–5 weeks after beneath renal...
We have identified a novel 69-kD peptide autoantigen (ICA69) associated with insulin-dependent diabetes mellitus (IDDM) by screening human islet lambda gt11 cDNA expression library cytoplasmic cell antibody positive sera from relatives of IDDM patients who progressed to the overt disease. The deduced open reading frame ICA69 predicts 483-amino acid protein. shows no nucleotide or amino sequence relation any known in GenBank, except for two short regions similarity BSA. probe hybridizes 2-kb...