A5100335540- Pancreatic function and diabetes
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Magnesium Alloys: Properties and Applications
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Enzyme Production and Characterization
- Nuclear Receptors and Signaling
- Aquaculture disease management and microbiota
- Advanced biosensing and bioanalysis techniques
- Marine Sponges and Natural Products
- Chronic Lymphocytic Leukemia Research
- Genomics and Phylogenetic Studies
- Receptor Mechanisms and Signaling
- Colorectal Cancer Treatments and Studies
- Diabetes Treatment and Management
- Aluminum Alloys Composites Properties
- Phytochemicals and Antioxidant Activities
- Aluminum toxicity and tolerance in plants and animals
- Microbial Natural Products and Biosynthesis
- Circular RNAs in diseases
- MicroRNA in disease regulation
Xiamen University
2016-2025
Institute of Food Science and Technology
2024-2025
Chinese Academy of Agricultural Sciences
2024-2025
Lanzhou University
2022-2025
Ministry of Agriculture and Rural Affairs
2022-2025
First Affiliated Hospital of Zhengzhou University
2025
Fujian Medical University
2025
East China University of Science and Technology
2025
Shandong Agricultural University
2025
Nanjing University
2023-2024
The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has radically changed targeted mutagenesis. Here, we present high-throughput mutagenesis pipeline using technology zebrafish that will make possible both saturation the genome and large-scale phenotyping efforts. We describe cloning-free single-guide RNA (sgRNA) synthesis, coupled with streamlined mutant identification methods utilizing fluorescent PCR multiplexed, sequencing. report germline transmission data from...
The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia air exposure. These traits may render L. a good model for investigating response mechanisms stress. To understand molecular genetic underlying adaptation stress, we sequenced assembled genome using bacterial...
This randomized, open-label trial compared the efficacy and safety of adjuvant
Abstract Background Subarachnoid hemorrhage (SAH) is a subtype of hemorrhagic stroke characterized by high mortality and low rates full recovery. This study aimed to investigate the epidemiological characteristics SAH between 1990 2021. Methods Data on incidence, mortality, disability-adjusted life-years (DALYs) from 2021 were obtained Global Burden Disease Study (GBD) Estimated annual percentage changes (EAPCs) calculated evaluate in age-standardized rate (ASR) incidence as well trends...
Abstract Determining the mechanism of gene function is greatly enhanced using conditional mutagenesis. However, generating engineered alleles inefficient and has only been widely used in mice. Importantly, multiplex mutagenesis requires extensive breeding. Here we demonstrate a system for one-generation zebrafish (Danio rerio) transgenic expression both cas9 multiple single guide RNAs (sgRNAs). We describe five distinct U6 promoters sgRNA efficient biallelic inactivation tyrosinase insulin...
4000 Background: In metastatic pancreatic cancer (PC), nab-P/G demonstrated significantly longer overall survival (OS) vs G. APACT assessed efficacy & safety of G in surgically resected PC. Methods: Treatment (tx)-naive patients (pts) with histologically confirmed PC, macroscopic complete resection, ECOG PS 0/1, CA19-9 < 100 U/mL were eligible. Stratification factors: resection status (R0/R1), lymph node (LN+/−), geographic region. Tx was initiated ≤ 12 wks postsurgery. Pts received...
Desmoplasia describes the deposition of extensive extracellular matrix and defines primary pancreatic ductal adenocarcinoma (PDA). The acellular component this stroma has been implicated in PDA pathogenesis is being targeted therapeutically clinical trials. By analyzing stromal content samples from numerous annotated data sets correlating with both anatomic site outcome, we found metastases liver, cause mortality to have less stroma, higher tumor cellularity than tumors. Experimentally...
The large yellow croaker (Pseudosciaena crocea) is an economically important marine fish in China. To understand the molecular basis for antiviral defense this species, we used Illumia paired-end sequencing to characterize spleen transcriptome of polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced croakers. library produced 56,355,728 reads and assembled into 108,237 contigs. As a result, 15,192 unigenes were found from transcriptome. Gene ontology analysis showed that 4,759 genes...
Localized drug delivery from drug-eluting stents (DESs) to target sites provides therapeutic efficacy with minimal systemic toxicity. However, DESs failure may cause thrombosis, delay arterial healing, and impede re-endothelialization. Bivalirudin (BVLD) nitric oxide (NO) promote healing. Nevertheless, it is difficult combine hydrophilic signal molecules hydrophobic antiproliferative drugs while maintaining their bioactivity. Here, we fabricated a micro- nanoscale network assembly consisting...
Ras undergoes interconversion between the active GTP-bound state and inactive GDP-bound state. This GTPase cycle, which controls activities of Ras, is accelerated by GTPase-activating proteins (GAPs) guanine nucleotide exchange factors (SOS). Oncogenic mutations could affect cycle impair functions. Additionally, Src-induced K-Ras Y32/64 dual phosphorylation has been reported to disrupt hinder downstream signaling. However, underlying mechanisms remain unclear. To address this, we performed...
Class B G protein-coupled receptors (GPCRs) are important targets in the treatment of metabolic syndrome and diabetes. Although multiple structures class GPCRs–G protein complexes have been elucidated, detailed activation mechanism remains unclear. Here, we combine Gaussian accelerated molecular dynamics simulations Markov state models (MSM) to investigate a canonical GPCR, human glucagon receptor–GCGR, including negative allosteric modulator-bound inactive state, agonist glucagon-bound...
Rationale: Previous studies have suggested that myocardial inflammation plays a critical role after ischemic infarction (MI); however, the underlying mechanisms still need to be fully elucidated. WW domain-containing ubiquitin E3 ligase 1 (WWP1) is considered as an important therapeutic target for cardiovascular diseases due its crucial function in non-ischemic cardiomyopathy, though it remains unknown whether targeting WWP1 can alleviate and injury post-MI. Methods: Recombinant...
Abstract Background Metabolic reprograming and immune escape are two hallmarks of cancer. However, how metabolic disorders drive in head neck squamous cell carcinoma (HNSCC) remains unclear. Therefore, the aim present study was to investigate landscape HNSCC its mechanism driving escape. Methods Analysis paired tumor tissues adjacent normal from 69 patients performed using liquid/gas chromatography‐mass spectrometry RNA‐sequencing. The tumor‐promoting function kynurenine (Kyn) explored vitro...