A5047062221- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Immune Cell Function and Interaction
- Diabetes Treatment and Management
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Metabolism, Diabetes, and Cancer
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Diet and metabolism studies
- Cannabis and Cannabinoid Research
- Pharmaceutical studies and practices
- Cellular transport and secretion
- Ferroptosis and cancer prognosis
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Neuroendocrine Tumor Research Advances
- Adrenal Hormones and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Pancreatitis Pathology and Treatment
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Celiac Disease Research and Management
- Pesticide Residue Analysis and Safety
Baylor College of Medicine
2014-2023
Vaxine (Australia)
2019
The Medical Center of Aurora
2019
Diabetes Research Center
2018
University of Michigan
2006-2015
University of Pittsburgh
1998-2011
Children's Hospital of Pittsburgh
1998-2011
Michigan United
2010
Ann Arbor Center for Independent Living
2010
Washtenaw Community College
2009
Islet cell antibodies (ICAs) are predictive of type I diabetes in first-degree relatives, but this immunohistochemical assay has proven difficult to standardize. As an alternative, we assessed the use radioassays for against three molecularly characterized islet autoantigens, including ICA512bdc (amino acid residues 256–979 IA-2 molecule, incorporating intracellular domain). We measured insulin autoantibodies (IAAs), GAD (GAAs), and (ICA512bdcAAs) by radioassay, addition ICAs, 882 relatives...
A substantial proportion of patients with adult-onset diabetes share features both type 1 (T1D) and 2 (T2D). These individuals, at diagnosis, clinically resemble T2D by not requiring insulin treatment, yet they have immunogenetic markers associated T1D. Such a slowly evolving form autoimmune diabetes, described as latent adults (LADA), accounts for 2–12% all though show considerable variability according to their demographics mode ascertainment. While therapeutic strategies aim metabolic...
OBJECTIVE—The aim of this study was to compare the prevalence being overweight in black and white children adolescents at onset insulin-treated diabetes during two time periods: 1979–1989 (period I) 1990–1998 II). RESEARCH DESIGN AND METHODS—All <19 years age diagnosed with treated insulin admitted Children’s Hospital Pittsburgh between January 1979 December 1998 were matched by sex, onset, year diagnosis. Data obtained from a review medical records. Overweight defined as BMI ≥85th...
We have identified a novel 69-kD peptide autoantigen (ICA69) associated with insulin-dependent diabetes mellitus (IDDM) by screening human islet lambda gt11 cDNA expression library cytoplasmic cell antibody positive sera from relatives of IDDM patients who progressed to the overt disease. The deduced open reading frame ICA69 predicts 483-amino acid protein. shows no nucleotide or amino sequence relation any known in GenBank, except for two short regions similarity BSA. probe hybridizes 2-kb...
Despite decades of acknowledging that a loss insulin-producing pancreatic β-cells is central to the disorder now referred as type 1 diabetes, specific roles for genetic susceptibility, environmental factors, immune system, and themselves in pathogenic processes underlying remain unclear (1,2). Looking back over this period, one can identify handful conceptualizations were seminal their attempt address issue, including posited by Dr. Gian Franco Bottazzo his 1986 article, “Death Beta Cell:...
Background Diabetes costs represent a large burden to both patients and the health care system. However, few studies that examine economic consequences of diabetes have distinguished between two major forms, type 1 2 diabetes, despite differences in underlying pathologies. Combining diseases implies there is no difference patient. In this study, we which often overlooked due larger population patients, compare them estimated reported literature. Methodology/Principal Findings Using...
OBJECTIVE Continuous glucose monitoring (CGM) parameters may identify individuals at risk for progression to overt type 1 diabetes. We aimed determine whether CGM metrics provide additional insights into clinical stage 3 RESEARCH DESIGN AND METHODS One hundred five relatives of in diabetes probands (median age 16.8 years; 89% non-Hispanic White; 43.8% female) from the TrialNet Pathway Prevention study underwent 7-day assessments and oral tolerance tests (OGTTs) 6-month intervals. The...
The most commonly used technical approach to isolate human pancreatic islets intended for allotransplants generates a product that is hampered by mechanical and chemical insults, which dramatically reduce the mass of viable functional transplantable cells. We tested novel class antioxidant compounds (SOD mimics: AEOL10113 AEOL10150) protect from oxidative stress in order improve preservation isolated tissue. Addition SOD mimic culture, after isolation, allowed survival significantly higher...
In light of an occurring growth elderly people affected by type 2 diabetes and recent observations indicating that may be a disease the innate immune system, we evaluated whether signs islet cell autoimmunity are associated with abnormal glucose control, presence insulin requirement, or activation acute-phase response in older individuals diabetes. GAD65 IA-2 autoantibodies along markers fibrinogen C-reactive protein were tested 196 serum samples from patients 94 nondiabetic control subjects...
As part of a general program screening islet expression libraries we have identified clone from λgt11 human library that reacts with diabetic sera and, upon sequencing, was determined to be the neuroendocrine autoantigen ICA512 (islet cell antigen 512). In current communication, describe development radioassay for autoantibodies ICA-512 (ICA512AA) using in vitro transcribed and translated protein production labeled antigen. Our initial results indicate this is significantly more sensitive...
Previous studies suggest that after 6 years of discordance, identical twin pairs rarely become concordant for type I diabetes. With up to 39 follow-up from the onset diabetes in index twin, we determined how many discordant twins have evidence β-cell autoimmunity and develop overt We longitudinally followed 23 (or triplets) were selected a total group 30 because they when first ascertained. Seven developed 3, 7, 8, 9, 31 36 discordance. By survival analysis, concordance 10 was estimated as...
Higher serum uric acid (SUA) is associated with diabetic kidney disease (DKD). Preventing Early Renal Loss in Diabetes (PERL) evaluates whether lowering SUA allopurinol slows glomerular filtration rate (GFR) loss people type 1 diabetes (T1D) and mild to moderate DKD. We present the PERL rationale, design, baseline characteristics.This double-blind, placebo-controlled, multicenter trial randomized 530 participants T1D, estimated GFR (eGFR) of 40-99.9 mL/min/1.73 m2, ≥4.5 m/dL, micro-...
Pancreatic ACE2 receptor expression, together with increased prevalence of insulin-requiring hyperglycemia in patients coronavirus disease 2019 (COVID-19), suggested that severe acute respiratory syndrome 2 (SARS-CoV-2) pancreatic infection might trigger a β-cell–selective inflammation precipitating autoimmune type 1 diabetes (T1D). We examined T1D incidence COVID-19 inside large, global population using “big data” approach. The 0–30-year-old confirmed over an ∼15-month period from the...