A5082039849- Diet and metabolism studies
- Bariatric Surgery and Outcomes
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- Birth, Development, and Health
- Dietary Effects on Health
- Adipose Tissue and Metabolism
- Developmental Biology and Gene Regulation
- Diabetes Treatment and Management
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Digestive system and related health
- Diet, Metabolism, and Disease
- Diabetes Management and Research
- Electrochemical sensors and biosensors
- Genomics and Chromatin Dynamics
- Gastric Cancer Management and Outcomes
- Immune Cell Function and Interaction
- Retinal Development and Disorders
- Prenatal Substance Exposure Effects
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- Regulation of Appetite and Obesity
Hebrew University of Jerusalem
2007-2025
Institute of Cancer Research
2023
Hadassah
2023
Hadassah Medical Center
2011-2013
During organogenesis, PAX6 is required for establishment of various progenitor subtypes within the central nervous system, eye and pancreas. expression maintained in a variety cell types each organ, although its role lineage how it acquires cell-specific activity remain elusive. Herein, we aimed to determine roles hierarchical organization PAX6-dependent gene regulatory network during differentiation retinal pigmented epithelium (RPE). Somatic mutagenesis Pax6 differentiating RPE revealed...
Diabetes is associated with increased risk for kidney disease, heart failure, and mortality. Sodium-glucose cotransporter 2 inhibitors (SGLT2i) prevent these adverse outcomes; however, the mechanisms involved are not clear. We generated a roadmap of metabolic alterations that occur in different organs diabetes response to SGLT2i. In vivo labeling 13C-glucose normoglycemic diabetic mice treated or without dapagliflozin, followed by metabolomics flux analyses, showed that, diabetes, glycolysis...
Bariatric surgery dramatically improves glycemic control, yet the underlying molecular mechanisms remain controversial because of confounding weight loss. We performed sleeve gastrectomy (SG) on obese and diabetic leptin receptor–deficient mice (db/db). One week postsurgery, weighed 5% less displayed improved glycemia compared with sham-operated controls, islets from SG reduced expression diabetes markers. month postsurgery more than preoperatively but remained near-euglycemic hepatic lipid...
RTK pathways establish cell fates in a wide range of developmental processes. However, how the pathway effector MAPK coordinately regulates expression multiple target genes is not fully understood. We have previously shown that EGFR causes phosphorylation and downregulation Groucho, global co-repressor widely used by many developmentally important repressors for silencing their various targets. Here, we use specific antibodies reveal dynamics Groucho MAPK, show phosphorylated response to...
Bariatric operations induce weight loss, which is associated with an improvement in hepatic steatosis and a reduction glucose production. It not clear whether these outcomes are entirely due to or the new anatomy imposed by surgery contributes metabolic function of liver. We performed vertical sleeve gastrectomy (VSG) on obese mice provided high-fat high-sucrose diet compared them weight-matched sham-operated (WMS). At 40 days after surgery, VSG-operated displayed less WMS. By measuring...
NK cells rapidly kill tumor cells, virus infected and even self cells. This is mediated via killer receptors, among which NKp46 (NCR1 in mice) prominent. We have recently demonstrated that type 1 diabetes (T1D) accumulate the diseased pancreas they manifest a hyporesponsive phenotype. In addition, we found recognizes an unknown ligand expressed by beta derived from humans mice blocking of activity prevented development. Here investigated properties ligand. show mainly located insulin...
Mice are a common model organism used to study metabolic diseases such as diabetes mellitus. Glucose levels typically measured by tail-bleeding, which requires handling the mice, causes stress, and does not provide data on freely behaving mice during dark cycle. State-of-the-art continuous glucose measurement in inserting probe into aortic arch of mouse, well specialized telemetry system. This challenging expensive method has been adopted most labs. Here, we present simple protocol involving...
Bariatric surgeries may lead to an improvement in metabolic fatty liver disease, and a reduction the levels of hepatic enzyme Alanine Aminotransferase (ALT). We compared effects Sleeve Gastrectomy (SG), Roux en Y Gastric Bypass (RYGB) One Anastomosis (OAGB) on ALT by analysis two-year follow-up data from 4980 patients Israeli Registry that included laboratory tests demographic information. Pre-operative characteristics patients, particularly enzymes, were similar across surgery types....
The Hox genes pattern the anterior-posterior axis in developing embryos through tightly regulated, partially overlapping, temporal and spatial expression domains. Initial regulation of is important to establish these overlapping transcription Cdx homeodomain factors have been proposed as regulators, but their precise role mechanism during this regulatory interaction remain unclear. In Xenopus embryos, HoxC8 transcripts begin accumulate mid/late gastrula. Cdx1 overexpression knockdown lead...
Gastrulation marks the onset of germ layer formation from undifferentiated precursor cells maintained by a network including Pou5f1 gene, Oct3/4. Negative regulation state is prerequisite for and subsequent development. A novel cross-regulatory was characterized Cdx1 genes as part signals controlling gastrulation. Of particular interest observation that, preceding gastrulation, Xenopus Oct3/4 factors, Oct60, Oct25, Oct91, positively regulate expression through FGF signaling, during...
Mothers that underwent bariatric surgery are at higher risk for delivering a small-for-gestational age (SGA) infant. This phenomenon is attributed to malabsorption and rapid weight loss following surgery. We compared pregnancy outcomes in lean mice sham or sleeve gastrectomy (SG). SG led reduction glucose levels an increase postprandial of glucagon-like peptide 1 (Glp1) without affecting during pregnancy. Pups SG-operated (SG pups) were born SGA. The placenta pancreas the pups not affected...
Patients that undergo bariatric surgery experience weight loss and a reduction in the plasma levels of hepatic enzymes alanine aminotransferase (ALT) aspartate (AST). We used Israeli national registry, which includes demographic, clinical, biochemical data on 19,403 patients, 1335 patients had two-year follow-up ALT, AST, A1C, BMI, to test dependence ALT AST loss. The were analyzed using regression models, retrospective matching, time course analyses. Changes liver did not correlate with...
A mouse model for one anastomosis gastric bypass (OAGB) surgery displays similar outcomes to clinical reports and enables study the weight loss-dependent -independent effects of this bariatric surgery.
Obesity and hyperglycemia are risk factors for cognitive decline the development of Alzheimer's Disease (AD). Bariatric surgery is an effective treatment obesity that was shown to improve in obese patients. exert weight loss independent effects on metabolic diseases such as type 2 diabetes. We tested whether sleeve gastrectomy (SG), a common bariatric surgery, can affect impairment lean, normoglycemic female 5xFAD mice, genetic model AD. mice wild-type (WT) littermates underwent SG or sham...
Bariatric surgeries induce weight loss which is associated with an improvement in hepatic steatosis and reduction glucose production. It not clear whether these outcomes are entirely due to weight-loss, or the new anatomy imposed by surgery contributes metabolic function of liver. We performed vertical sleeve gastrectomy (VSG) on obese mice provided a high-fat high-sucrose diet, compared them diet weight-matched sham-operated (WMS). 40 days after surgery, VSG-operated displayed lesser WMS....
Mice are a common model organism used to study metabolic diseases such as diabetes mellitus. Glucose levels typically measured by tail-bleeding, which requires handling the mice, causes stress, and does not provide data on freely behaving mice during dark cycle. State-of-the-art continuous glucose measurement in inserting probe into aortic arch of mouse, well specialized telemetry system. This challenging expensive method has been adopted most labs. Here, we present simple protocol involving...
Abstract Bariatric surgery is an effective obesity treatment, leading to weight loss and improvement in glycemia, that characterized by hypersecretion of gastrointestinal hormones. However, regain relapse hyperglycemia are not uncommon. Here, we investigated the role somatostatin (Sst) bariatric outcomes using a mouse model sleeve gastrectomy (SG). Sst knockout (sst-ko) mice fed with calorie-rich diet gained normally, had mild favorable metabolic phenotype compared heterozygous sibling...
Weight loss interventions, including dietary changes, pharmacotherapy, or bariatric surgery, prevent many of the adverse consequences obesity, and may also confer intervention-specific benefits beyond those seen with decreased weight alone. We compared molecular effects different interventions on liver metabolism to understand mechanisms underlying these benefits. Male rats a high-fat, high-sucrose diet underwent sleeve gastrectomy (SG) intermittent fasting caloric restriction (IF-CR),...