A5076247390- Liver physiology and pathology
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Liver Disease Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- Pancreatic and Hepatic Oncology Research
- Renal and related cancers
- Virus-based gene therapy research
- Digestive system and related health
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- FOXO transcription factor regulation
- Genetic and Kidney Cyst Diseases
- Epigenetics and DNA Methylation
- Pediatric Hepatobiliary Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Caveolin-1 and cellular processes
- Biomedical Ethics and Regulation
- Diabetes Management and Research
- Cancer, Hypoxia, and Metabolism
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Tissue Engineering and Regenerative Medicine
University of Cambridge
2014-2025
Max Planck Institute of Molecular Cell Biology and Genetics
2019-2025
The Gurdon Institute
2014-2025
Medical Research Council
2015-2023
Wellcome/MRC Cambridge Stem Cell Institute
2015-2023
Queen Mary University of London
2023
William Harvey Research Institute
2023
Center for Systems Biology Dresden
2023
TU Dresden
2023
Wellcome Trust
2014-2022
Despite the enormous replication potential of human liver, there are currently no culture systems available that sustain hepatocyte and/or function in vitro. We have shown previously single mouse Lgr5+ liver stem cells can be expanded as epithelial organoids vitro and differentiated into functional hepatocytes vivo. now describe conditions allowing long-term expansion adult bile duct-derived bipotent progenitor from liver. The highly stable at chromosome structural level, while base changes...
Background & AimsWe previously established long-term, 3-dimensional culture of organoids from mouse tissues (intestine, stomach, pancreas, and liver) human intestine pancreas. Here we describe conditions required for long-term gastric stem cells. The technology can be applied to study the epithelial response infection with Helicobacter pylori.MethodsWe generated surgical samples corpus. Culture were developed based on those intestinal systems. We used microinjection infect H pylori....
Organoids have attracted increasing attention because they are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function corresponding vivo tissue. They can be dissected interrogated for fundamental mechanistic studies on development, regeneration, repair human tissues. also used diagnostics, disease modeling, drug discovery, personalized medicine. derived from either pluripotent or tissue-resident stem (embryonic adult)...
Multipotent stem cells and their lineage-restricted progeny drive nephron formation within the developing kidney. Here, we document expression of adult cell marker Lgr5 in kidney assess stem/progenitor identity Lgr5+ve via vivo lineage tracing. The appearance localization coincided with that S-shaped body around embryonic day 14. remained restricted to clusters nephrons cortex until postnatal 7, when was permanently silenced. In tracing identified as a population nascent dedicated generating...
The molecular events that drive hepatitis B virus (HBV)-mediated transformation and tumorigenesis have remained largely unclear, due to the absence of a relevant primary model system. Here we propose use human liver organoids as platform for modeling HBV infection related tumorigenesis. We first describe ex vivo HBV-infection derived from healthy donor after challenge with recombinant or HBV-infected patient serum. produced covalently closed circular DNA (cccDNA) early antigen (HBeAg),...
To address the long-known relationship between supernumerary centrosomes and cancer, we have generated a transgenic mouse that permits inducible expression of master regulator centriole duplication, Polo-like-kinase-4 (Plk4). Over-expression Plk4 from this transgene advances onset tumour formation occurs in absence suppressor p53. over-expression also leads to hyperproliferation cells pancreas skin is enhanced p53 null background. Pancreatic islets become enlarged following as result equal...