A5067217695- Metal complexes synthesis and properties
- Hepatitis B Virus Studies
- Ferrocene Chemistry and Applications
- Peptidase Inhibition and Analysis
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Chromatin Remodeling and Cancer
- Pancreatic and Hepatic Oncology Research
- Bladder and Urothelial Cancer Treatments
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Berberine and alkaloids research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Hepatitis C virus research
- Pancreatic function and diabetes
- Drug-Induced Hepatotoxicity and Protection
- Cancer-related gene regulation
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Radiopharmaceutical Chemistry and Applications
- Lanthanide and Transition Metal Complexes
- Malaria Research and Control
Erasmus MC
2019-2025
Erasmus MC Cancer Institute
2025
Erasmus University Rotterdam
2023-2024
The molecular events that drive hepatitis B virus (HBV)-mediated transformation and tumorigenesis have remained largely unclear, due to the absence of a relevant primary model system. Here we propose use human liver organoids as platform for modeling HBV infection related tumorigenesis. We first describe ex vivo HBV-infection derived from healthy donor after challenge with recombinant or HBV-infected patient serum. produced covalently closed circular DNA (cccDNA) early antigen (HBeAg),...
Organoids as self-organized structure derived from stem cells can recapitulate the function of an organ in miniature form which have developed great potential for clinical translation, drug screening and personalized medicine. Nevertheless, majority patient-derived organoids (PDOs) are currently being cultured basement membrane matrices (BMMs), constrained by xenogeneic origin, batch-to-batch variability, cost, complexity. Besides, organoid culture relies on biochemical signals provided...
There is an unmet need to improve the efficacy of platinum-based cancer chemotherapy, which used in primary and metastatic settings many types. In bladder cancer, chemotherapy leads better outcomes a subset patients when neoadjuvant setting or combination with immunotherapy for advanced disease. Despite such promising results, extending benefits platinum drugs greater number highly desirable. Using multiomic assessment cisplatin-responsive -resistant human cell lines whole-genome CRISPR...
Abstract The molecular events that drive Hepatitis B virus (HBV)-mediated transformation and tumorigenesis have remained largely unclear, due to the absence of a relevant primary model system. Here we propose use human liver organoids as platform for modeling HBV infection related tumorigenesis. We first describe ex vivo HBV-infection derived from healthy donor after challenge with recombinant or HBV-infected patient serum. infected produced cccDNA, expressed intracellular RNA proteins,...
Hepatitis B virus (HBV) infection represents a major public health problem infecting approximately 400 million people worldwide. Despite the availability of preventive vaccine and anti-viral therapies, chronic HBV remains issue because it increases risk developing liver cirrhosis hepatocellular carcinoma (HCC). The lack relevant in vitro model for study molecular mechanisms that drive replication latency, as well HBV-related carcinogenesis, has been one obstacles to development curative...
Somatic mutations in ARID1A (AT-rich interactive domain-containing protein 1A) are present approximately 25% of bladder cancers (BC) and associated with poor prognosis. With a view to discover effective treatment options for ARID1A-deficient BC patients, we set out identify targetable effectors dysregulated consequent deficiency. Integrative analyses depletion normal organoids data mining publicly available datasets revealed upregulation DNA repair cell cycle-associated genes loss identified...
A major unmet clinical need is a therapeutic capable of removing hepatitis B virus (HBV) genome from the liver infected individuals to reduce their risk developing cancer. strategy deliver such therapy could utilize ability target and promote apoptosis hepatocytes. Presently there no clinically relevant that has been shown effectively remove persistent episomal covalently closed circular HBV DNA (cccDNA) nucleus We used linearized single length various genotypes establish cccDNA-like...
The absence of long term, primary untransformed in vitro models that support hepatitis B virus (HBV) infection and replication have hampered HBV pre-clinical research, which was reflected the a curative therapy until recently.One limitations for research has been high titer pure recombinant stocks, which, as we describe here, can be generated using simple, reproducible protocols.In addition to more conventional vivo liver model systems, purified stocks also used efficiently infect...
Malaria, a major public health burden, is caused by
<p>Supplementary Figures and Supplementary Table legends.</p>
<p>Supplementary Figures and Supplementary Table legends.</p>
<div>Abstract<p>There is an unmet need to improve the efficacy of platinum-based cancer chemotherapy, which used in primary and metastatic settings many types. In bladder cancer, chemotherapy leads better outcomes a subset patients when neoadjuvant setting or combination with immunotherapy for advanced disease. Despite such promising results, extending benefits platinum drugs greater number highly desirable. Using multiomic assessment cisplatin-responsive -resistant human cell...
<div>Abstract<p>There is an unmet need to improve the efficacy of platinum-based cancer chemotherapy, which used in primary and metastatic settings many types. In bladder cancer, chemotherapy leads better outcomes a subset patients when neoadjuvant setting or combination with immunotherapy for advanced disease. Despite such promising results, extending benefits platinum drugs greater number highly desirable. Using multiomic assessment cisplatin-responsive -resistant human cell...
<title>Abstract</title> Organoids as an aggregation of stem cells can recapitulate the function organs in miniature form and have developed great potential for clinical translation, drug screening personalized medicine over last decade. Most organoids are currently cultured basement membrane matrices (BMMs), which is hampered by xenogeneic origin, batch-to-batch variability, cost complexity. In addition, organoid culture relies on biochemical signals provided various growth factors...
ABSTRACT There is an unmet need to improve efficacy of platinum-based cancer chemotherapy. Using multi-omic assessment cisplatin-responsive and -resistant human bladder cell lines whole-genome CRISPR screens, we identified Puromycin-Sensitive Aminopeptidase, NPEPPS, as a novel driver cisplatin resistance. NPEPPS depletion sensitizes resistant cells in vitro vivo . Conversely, overexpression sensitive increased We show that affects treatment response by regulating intracellular...
Malaria, a major public health burden, is caused by Plasmodium spp parasites that first replicate in the human liver to establish infection before spreading erythrocytes. Liver-stage malaria research has remained challenging due lack of clinically relevant and scalable vitro model liver. Here, we demonstrate organoids derived from intrahepatic ductal cells differentiated into hepatocyte-like fate can support maturation falciparum. The P. falciparum exoerythrocytic forms observed expressed...
Abstract Introduction and Objectives: Cisplatin-based neoadjuvant chemotherapy (NAC) is the recommended treatment for muscle-invasive bladder cancer (MIBC) patients. Patients with a pathological complete response after NAC (pCR), have good 5-yr OS of 80%. However, due to resistance, only 25% patients achieve pCR. Our aim increase number reaching pCR by overcoming cisplatin resistance. Previously we identified NPEPPS as key player in resistance that prevents uptake via volume-regulated anion...