A5052116403- Cancer Cells and Metastasis
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Angiogenesis and VEGF in Cancer
- Phagocytosis and Immune Regulation
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory mediators and NSAID effects
- Genetic factors in colorectal cancer
- Digestive system and related health
- Wnt/β-catenin signaling in development and cancer
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Estrogen and related hormone effects
- Congenital gastrointestinal and neural anomalies
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Research and Treatments
Erasmus MC
2015-2025
Erasmus University Rotterdam
2009-2025
Erasmus MC Cancer Institute
2015-2025
University of Chieti-Pescara
2004-2023
Meertens Institute
2012-2023
Rotterdam University of Applied Sciences
2020
Shanghai Jiao Tong University
2016
XinHua Hospital
2016
University Medical Center Utrecht
2016
Thorax Foundation
2016
IBD syndromes such as Crohn's disease and ulcerative colitis result from the inflammation of specific intestinal segments. Although many studies have reported on regenerative response progenitor stem cells to tissue injury, very little is known about differentiated lineages inflammatory cues. Here, we show that acute mouse small intestine followed by a dramatic loss Lgr5+ cells. Instead, Paneth re-enter cell cycle, lose their secretory expression signature, acquire stem-like properties, thus...
Trop-2/EGP-1/GA733-1 is a recently identified cell surface glycoprotein highly expressed by human carcinomas. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites phosphatidyl-inositol binding consensus sequence. Thus, we investigated whether might be functional signaling molecule. Using the fluorescent probe Fura-2, assayed calcium levels in cancer cells stimulated with anti-Trop-2 or control antibodies. Three MAbs, Rs7-7G11, MOv16 162-46.2...
Adult stem cell niches are often co-inhabited by cycling and quiescent cells. In the intestine, lineage tracing has identified Lgr5(+) cells as frequently cells, whereas Bmi1(+), mTert(+), Hopx(+) Lrig1(+) appear to be more quiescent. Here, we have applied a non-mutagenic cycle independent approach isolate characterize small intestinal label-retaining (LRCs) persisting in lower third of crypt Lieberkühn for up 100 days. LRCs do not express markers proliferation enterocyte, goblet or...
Abstract Objective: A high percentage of grade II and III gliomas have mutations in the gene encoding isocitrate dehydrogenase ( IDH1 ). This mutation is always a heterozygous point that affects amino acid arginine at position 132 results loss its native enzymatic activity gain alternative (producing D‐2‐hydroxyglutarate). The objective this study was to investigate cellular effects R132H . Methods: Functional consequences were examined among others using fluorescence‐activated cell sorting,...
Phenotypic plasticity represents the most relevant hallmark of carcinoma cell as it bestows with capacity transiently altering its morphological and functional features while
Abstract According to conventional views, colon cancer originates from stem cells. However, inflammation, a key risk factor for cancer, has been shown suppress intestinal stemness. Here, we used Paneth cells as model assess the capacity of differentiated lineages trigger tumorigenesis in context inflammation mice. Upon cell-specific Apc mutations led tumors reminiscent not only those arising patients with inflammatory bowel disease, but also larger fraction human sporadic cancers. The latter...
Aspirin affords cardioprotection through the acetylation of serine 529 in human cyclooxygenase-1 (COX-1) anucleated platelets, inducing a permanent defect thromboxane A 2 (TXA )–dependent platelet function. However, heterogeneity COX-1 suppression by aspirin has been detected cardiovascular disease and may contribute to failure prevent clinical events. The recent recognized capacity platelets make proteins de novo paves way identify new mechanisms involved variable response aspirin. We found...
Cancer stem-like cells (CSC) may be critical to maintain the malignant behavior of solid and hematopoietic cancers. Recently, patients with endometrial cancer whose tumors expressed high levels aldehyde dehydrogenase (ALDH), a detoxifying enzyme characteristic many progenitor stem cells, exhibited relative reduction in survival compared low ALDH. Given evidence its role as CSC marker, we hypothesized that level ALDH activity (ALDH(hi)) tumor might positively correlate presence stem-...
Canonical Wnt signaling plays a rate-limiting role in regulating self-renewal and differentiation mouse embryonic stem cells (ESCs). We have previously shown that mutation the Apc (adenomatous polyposis coli) tumor suppressor gene constitutively activates ESCs inhibits their capacity to differentiate towards ecto-, meso-, endodermal lineages. However, underlying molecular cellular mechanisms through which regulates lineage remain date largely unknown. To this aim, we derived studied...
The gross majority of colorectal cancer cases results from aberrant Wnt/β-catenin signalling through adenomatous polyposis coli (APC) or CTNNB1 mutations. However, a subset human colon tumours harbour, mutually exclusive with APC and mutations, gene fusions in RSPO2 RSPO3, leading to enhanced expression these R-spondin genes. This suggested that RSPO activation can substitute for the most common mutations as an alternative driver intestinal cancer. Involvement RSPO3 tumour growth was...
The majority of glioma-associated microglia/macrophages have been identified as M2-type macrophages with immune suppressive and tumor supportive action. Recently, the extracellular adenosine deaminase protein Cat Eye Syndrome Critical Region Protein 1 (CECR1) was shown to regulate macrophage maturation. In this study, we investigate role CECR1 in regulation response.Expression assessed human glioma samples. CECR1-mediated response studied vitro, using donor derived CD14+ monocytes THP-1...
Abstract Phenotypic plasticity, defined as the ability of individual cells with stable genotypes to exert different phenotypes upon exposure specific environmental cues, represent quintessential hallmark cancer cell en route from primary lesion distant organ sites where metastatic colonization will occur. plasticity is driven by a broad spectrum epigenetic mechanisms that allow for reversibility epithelial-to-mesenchymal and mesenchymal-to-epithelial transitions (EMT/MET). By taking...
In squamous cell carcinoma, the levels of nitric oxide (NO) derived from inducible NO synthase (iNOS) and prostaglandin E2 (PGE2) cyclooxygenase-2 (COX-2) originated tumor cells or tumor-associated inflammatory have been reported to correlate with growth, metastasis, angiogenesis. The present study examined role iNOS signaling pathway in PGE2-mediated invasiveness proliferation A431, SCC-9 cells. Cell invasion promoted by PGE2 were blocked silencing RNA iNOS/guanylate cyclase (GC)...
Prostate cancer consists of secretory cells and a population immature cells. The function their mutual relation with are still poorly understood. Immature either have hierarchical to (stem cell model) or represent an inducible emerging upon appropriate stimulation differentiated Hepatocyte Growth Factor (HGF) receptor c-MET is specifically expressed in prostate Our objective determine the role by analysis HGF/c-MET pathway. Gene-expression profiling DU145 stimulated HGF revealed induction...
In fertile women, the endometrium undergoes regular cycles of tissue build-up and regression. It is likely that uterine stem cells are involved in this remarkable turn over. The main goal our current investigations was to identify slow-cycling (quiescent) endometrial by means a pulse-chase approach selectively earmark, prospectively isolate, characterize label-retaining (LRCs). To aim, transgenic mice expressing histone2B-GFP (H2B-GFP) Tet-inducible fashion were administered doxycycline...
Inhibition of AKT is a key target area for personalized cancer medicine. However, predictive markers response to inhibitors are lacking. Correspondingly, the AKT-dependent chain command tumor growth, which will mediate therapeutic responses, remains unclear.Proteomic profiling was utilized identify nodal hubs Trop-2 growth-driving network. Kinase-specific were used dissect Trop-2-dependent from Trop-2-independent pathways. In vitro assays, in vivo preclinical models, and case series primary...
The recent approval of pembrolizumab in recurrent or metastatic cervical cancer warrants further investigations into the usefulness immunotherapies for more durable and less radical interventions. In this study, targeting potential anti-PD-L1-functionalized immunoliposomes was tested a 3D vitro cancer-on-a-chip model. Immunolipsomes were synthesized decorated externally with monovalent anti-PD-L1 Fab' fragments commercially available atezolizumab. Cervical cell lines varying levels PD-L1...
Isolation of neurons and glial cells from the enteric nervous system (ENS) enables ex-vivo studies, including analysis genomic transcriptomic profiles. While we previously reported a fluorescence activated cell sorting (FACS)-based isolation protocol for human ENS cells, no equivalent exists mice. As directly applying to mouse tissue, resulted in low recovery live compared different protocols optimize tissue dissociation colons. A 30-minute Liberase-based digestion showed optimal viable with...
Wnt signaling plays a central role in mammary stem cell (MaSC) homeostasis and breast cancer. In particular, epigenetic alterations at different members of the pathway have been identified among triple-negative, basal-like cancers. Previously, we developed mouse model for metaplastic adenocarcinoma, subtype triple-negative cancer, by targeting hypomorphic mutations endogenous Apc gene (Apc1572T/+). Here, employing CD24 CD29 surface antigens, subpopulation cancer cells (MaCSCs) from...