A5010797456- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- Hormonal and reproductive studies
- Prostate Cancer Diagnosis and Treatment
- Estrogen and related hormone effects
- Cancer Cells and Metastasis
- Mass Spectrometry Techniques and Applications
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Neuropeptides and Animal Physiology
- Cancer Treatment and Pharmacology
- Inflammatory mediators and NSAID effects
- Cancer Genomics and Diagnostics
- Xenotransplantation and immune response
- Cancer, Hypoxia, and Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA Interference and Gene Delivery
- Antioxidant Activity and Oxidative Stress
- Microfluidic and Bio-sensing Technologies
- PARP inhibition in cancer therapy
- Cancer-related gene regulation
Erasmus University Rotterdam
2015-2025
Erasmus MC
2016-2025
Erasmus MC Cancer Institute
2014-2024
John Wiley & Sons (United States)
2018-2019
Hudson Institute
2018-2019
Codarts Rotterdam
2013-2015
Meertens Institute
1999-2011
University of Fukui
2011
Rotterdam University of Applied Sciences
1993-2009
Baylor College of Medicine
1996
Abstract Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery validation. Here, 2D culture 3D mono- stromal co-culture increasing have been established cross-comparisons made using standard carcinoma lines: MCF7, LNCaP, NCI-H1437. Fluorescence-based growth curves, image analysis, immunohistochemistry treatment responses showed that end points...
Recently, a unique fusion between the prostate-specific, androgen-regulated TMPRSS2 gene and ETS genes ERG, ETV1, or ETV4 has been described in clinical prostate cancer. We investigated mechanisms of expression four genes, ETV4, FLI1, 11 xenografts representing different stages All five androgen-dependent showed as major transcript overexpression two splice variants TMPRSS2:ERG, linking exon 1 2 sequences to ERG 4. In one androgen-sensitive xenografts, transcripts ETV1 were detected....
Abstract The present work focused on the potential involvement of selective adaptations androgen receptor pathway in initiation and progression prostate cancer. We defined by selecting 200 genes that were responsive cancer cell lines and/or xenografts. This gene signature was then used for profiling xenografts patient-derived samples. Approximately half up-regulated well-differentiated compared with normal prostate. Functionally distinct parts specifically down-regulated high-grade cancers....
Androgen-deprivation therapy for prostate cancer (PC) eventually leads to castration-resistant PC (CRPC). Intratumoral androgen production might contribute tumor progression despite suppressed serum concentrations. In the present study, we investigated whether or CRPC tissue may be capable of intratumoral synthesis. Steroidogenic enzyme mRNAs were quantified in hormonally manipulated human cell lines and xenografts as well samples normal prostate, locally confined advanced PC, local...
Prostate-specific membrane antigen (PSMA) is overexpressed in prostate cancer (PCa) and a promising target for molecular imaging therapy. Nanobodies (single-domain antibodies, V<sub>HH</sub>) are the smallest antibody-based fragments possessing ideal properties, such as high specificity rapid background clearance. We developed novel anti-PSMA Nanobody (JVZ-007) targeted therapy of PCa. Here, we report on application <sup>111</sup>In-radiolabeled SPECT/CT <b>Methods:</b> A library was...
Precision-cut slices of in vivo tumours permit interrogation vitro heterogeneous cells from solid together with their native microenvironment. They offer a low throughput but high content experimental platform. Using mouse models as surrogates for three common human tumours, we describe standardised workflow systematic comparison tumour slice cultivation methods and tissue microarray-based method to archive them. Cultivated were compared source using immunohistochemical transcriptional...
Prostate-specific membrane antigen (PSMA) is a well-established target for nuclear imaging and therapy of prostate cancer (PCa).Radiolabeled small-molecule PSMA inhibitors are excellent candidates PCa theranostics-they rapidly efficiently localize in tumor lesions.However, high tracer uptake kidneys salivary glands major concerns therapeutic applications.Here, we present the preclinical application I&T, DOTAGA-chelated urea-based inhibitor, SPECT/CT radionuclide PCa. 111 In-PSMA I&T showed...
Background Prostate cancer is initially dependent on androgens for survival and growth, making hormonal therapy the cornerstone treatment late-stage tumors. However, despite initial remission, will inevitably recur. The present study was designed to investigate how androgen-dependent prostate cells eventually survive resume growth under androgen-deprived antiandrogen supplemented conditions. As model system, we used androgen-responsive PC346C cell line its therapy-resistant sublines:...
Novel markers for prostate cancer (PCa) are needed because current established such as prostate-specific antigen lack diagnostic specificity and prognostic value. Proteomics analysis of serum from mice grafted with human PCa xenografts resulted in the identification 44 tumor-derived proteins. Besides secreted proteins we identified several cytoplasmic proteins, among which were most subunits proteasome. Native gel electrophoresis sandwich ELISA showed that these present proteasome complexes...
Background While it has been challenging to establish prostate cancer patient‐derived xenografts (PDXs), with a take rate of 10‐40% and long latency time, multiple groups throughout the world have developed methods for successful establishment serially transplantable human PDXs using variety immune deficient mice. In 2014, Movember Foundation launched Global Action Plan 1 (GAP1) project support an international collaborative PDX program involving eleven groups. Between these consortium...
Abstract Purpose Various radiolabeled prostate-specific membrane antigen (PSMA)–targeting tracers are clinically applied for prostate cancer (PCa) imaging and targeted radionuclide therapy. The PSMA binding affinities, biodistribution, DNA-damaging capacities of these radiotracers have not yet been compared in detail. A major concern PSMA-targeting is the toxicity other PSMA-expressing organs, such as salivary glands, thus demanding careful evaluation most optimal safest radiotracer. In this...
Clinical and preclinical studies have revealed that alterations in DNA damage response (DDR) pathways may play an important role prostate cancer (PCa) etiology progression. These can influence PCa responses to radiotherapy anti-androgen treatment. The identification of repair gene aberrations has driven the interest for further evaluation whether these genetic changes serve as biomarkers patient stratification.In this review, we summarize current knowledge on DDR PCa, their potential impact...