A5088738771- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- AI in cancer detection
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Radiomics and Machine Learning in Medical Imaging
- Cancer Genomics and Diagnostics
- Autoimmune Bullous Skin Diseases
- Nanoplatforms for cancer theranostics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune cells in cancer
- Cell Image Analysis Techniques
- Electrocatalysts for Energy Conversion
- Generative Adversarial Networks and Image Synthesis
- Digital Imaging for Blood Diseases
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Cancer, Hypoxia, and Metabolism
- Multiple Sclerosis Research Studies
- Urticaria and Related Conditions
- Metalloenzymes and iron-sulfur proteins
- HER2/EGFR in Cancer Research
- CAR-T cell therapy research
Charles River Laboratories (Germany)
2017-2024
Ludwig-Maximilians-Universität München
2021-2024
Charles River Laboratories (Netherlands)
2019-2024
Universität Ulm
2022-2023
Munich Cluster for Systems Neurology
2023
Koç University
2023
University of Tübingen
2021
LMU Klinikum
2021
University of Freiburg
2009-2014
University Medical Center Freiburg
2008-2012
Allergic contact dermatitis and its animal model, hypersensitivity (CHS), are T cell–mediated inflammatory skin diseases induced by allergens. Though numerous cellular molecular players known, the mechanism of chemical-induced sensitization remains poorly understood. Here, we identify neutrophils as crucial in phase CHS. Genetic deficiency caused myeloid-specific deletion Mcl-1 or antibody-mediated depletion before abrogated CHS response. Neutrophil reduced allergen-induced cytokine...
Precision-cut slices of in vivo tumours permit interrogation vitro heterogeneous cells from solid together with their native microenvironment. They offer a low throughput but high content experimental platform. Using mouse models as surrogates for three common human tumours, we describe standardised workflow systematic comparison tumour slice cultivation methods and tissue microarray-based method to archive them. Cultivated were compared source using immunohistochemical transcriptional...
Antibodies to CD20 efficiently reduce new relapses in multiple sclerosis (MS), and ocrelizumab has been shown be effective also primary progressive MS. Although anti-CD20 treatments deplete B cells blood, some
Cobaloximes are promising, earth-abundant catalysts for the light-driven hydrogen evolution reaction (HER). Typically, these cobalt(III) complexes prepared in situ or employed their neutral form, example, [Co(dmgH)2 (py)Cl], even though related complex salts have been reported previously and could, principle, offer improved catalytic activity as well more efficient immobilization on solid support. Herein, we report an interdisciplinary investigation into (py)2 ]+ [Co(dmgBPh2 )2 Cl2 ]- ,...
Abstract Introduction In Multiple Sclerosis (MS), patients´ characteristics and (bio)markers that reliably predict the individual disease prognosis at onset are lacking. Cohort studies allow a close follow-up of MS histories thorough phenotyping patients. Therefore, multicenter cohort study was initiated to implement wide spectrum data in newly diagnosed Methods ProVal-MS (Prospective validate multidimensional decision score predicts treatment outcome 24 months untreated patients with...
Bullous pemphigoid is an autoimmune blistering skin disease associated with autoantibodies against the dermal-epidermal junction. Passive transfer of antibodies BP180/collagen (C) XVII, a major hemidesmosomal antigen, into neonatal mice results in separation upon applying gentle pressure to their skin, but not spontaneous blistering. In addition, this mouse model precludes treatment and observation diseased animals beyond 2-3 days. Therefore, present study we have developed new reproducing...
In up to 30% of non-small cell lung cancer (NSCLC) patients, the oncogenic driver tumor growth is a constitutively activated epidermal factor receptor (EGFR). Although these patients gain great benefit from treatment with EGFR tyrosine kinase inhibitors, development resistance inevitable. To model emergence drug resistance, an EGFR-driven, patient-derived xenograft (PDX) NSCLC was treated continuously Gefitinib in vivo. Over period more than three months, separate clones developed and were...
Abstract To date most studies focusing on light‐driven water splitting for green energy conversion schemes depend bulk analyses of the produced hydrogen using, e. g ., head‐space gas chromatography. In this contribution, we present fabrication Pd‐modified microelectrodes in situ electrochemical measurements using de‐alloyed Au−Ni microelectrodes. Stability and pH‐dependent investigations revealed stability these microsensors over a period seven days, confirmed their applicability pH range...
Background The field of cancer immunology is rapidly moving towards innovative therapeutic strategies, resulting in the need for robust and predictive preclinical platforms reflecting immunological response to cancer. Well characterized models are essential development biomarkers oncology as well immune-oncology space. In current study, gold standard being refined combined with novel image analysis tools meet those requirements. Methods A panel 14 non-small cell lung patient-derived...
Abstract Relevant in vivo and vitro cancer models have been invaluable for our current understanding of biology, as well therapy development. Using appropriate the drug development process significantly supports reliable swift decision making proceeding along value chain. In parallel, animal experiments can be designed more specific to scientific regulatory needs, resulting shorter cycles eventually availability better therapies patients. Patient-derived xenografts (PDX) are generated by...
Abstract Organotypic, three-dimensional (3D) cancer models have enabled investigations of complex microtissues in increasingly realistic conditions. However, a drawback these advanced remains the poor biological relevance cell lines, while higher clinical significance would be obtainable with patient-derived cultures. Here, we describe generation and data analysis 3D microtissue from xenografts (PDX) non-small lung carcinoma (NSCLC). Standard care anti-cancer drugs were applied altered...
We propose a deep learning workflow for the classification of hematoxylin and eosin stained histological whole-slide images non-small-cell lung cancer. The includes automatic extraction meta-features characterization tumor. show that tissue-classification produces state-of-the-art results with an average F1-score 83%. Manual supervision indicates experts, in practice, accept far higher percentage predictions. Furthermore, extracted are validated via visualization revealing relevant...
<h3>Objectives</h3> To evaluate the long-term effects of natalizumab (NTZ) on different features intrathecal immunoglobulin (Ig) synthesis in patients with multiple sclerosis (MS) and to quantify expression α4-integrin stages B-cell maturation. <h3>Methods</h3> We combined a cross-sectional (49 NTZ-treated MS patients, mean treatment duration 5.1 years, 47 untreated patients) longitudinal study (33 before during NTZ, duration: 4.8 years), analyzing paired serum CSF samples for IgG, IgA, IgM...
Background: Data on the humoral vaccine response in patients anti-interleukin-6 (IL-6) receptor therapy remain scarce. Objective: The main objective of our study was to investigate after vaccination against SARS-CoV-2 neuromyelitis optica spectrum disorder (NMOSD)/myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) treated with anti-IL-6 therapy. Secondarily, we analyzed relapse activity timely associated vaccination. Methods: In this retrospective cross-sectional...
Rodent models have contributed significantly to the understanding of haematological malignancies. One important model system in this context are patient-derived xenografts (PDX). In current study, we examined 20 acute leukaemia PDX for growth behaviour, infiltration haemopoietic organs and sensitivity towards cytarabine. were injected intratibially (i.t.), intrasplenicaly (i.s.) or subcutaneously (s.c.) into immune compromised mice. For 18/20 engraftment capacity was independent implantation...