A5075149163- Multiple Sclerosis Research Studies
- Peripheral Neuropathies and Disorders
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Hereditary Neurological Disorders
- Rheumatoid Arthritis Research and Therapies
- Polyomavirus and related diseases
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Ocular Diseases and Behçet’s Syndrome
- RNA Research and Splicing
- Peripheral Nerve Disorders
- Central Venous Catheters and Hemodialysis
- Psoriasis: Treatment and Pathogenesis
- Glaucoma and retinal disorders
- Intracerebral and Subarachnoid Hemorrhage Research
- Chronic Lymphocytic Leukemia Research
- Drug-Induced Adverse Reactions
- Neurogenesis and neuroplasticity mechanisms
- Myasthenia Gravis and Thymoma
- Parkinson's Disease and Spinal Disorders
- Ultrasound and Hyperthermia Applications
University of Tübingen
2015-2024
Hertie Institute for Clinical Brain Research
2015-2024
University Children's Hospital Tübingen
2019
German Center for Neurodegenerative Diseases
2016
University of Würzburg
2014
To analyze whether 1 of the 2 apheresis techniques, therapeutic plasma exchange (PE) or immunoadsorption (IA), is superior in treating neuromyelitis optica spectrum disorder (NMOSD) attacks and to identify predictive factors for complete remission (CR).This retrospective cohort study was based on registry German Neuromyelitis Optica Study Group, a nationwide network established 2008. It recruited patients with diagnosed according 2006 Wingerchuk criteria aquaporin-4...
Abstract Introduction Molecular biomarkers for Alzheimer's disease (AD) can support detection and improved care patients, but novel candidates require verification. We previously reported a 12‐micro RNA (miRNA) blood‐based signature using next‐generation sequencing (NGS) of 54 AD cases 22 controls. Methods performed validation 49 55 controls NGS also included 20 mild cognitive impairment 90 multiple sclerosis samples to identify nonspecific markers. Thus, 103 cases, 77 unaffected controls,...
Double negative (DN) (CD19 + CD20 low CD27 - IgD ) B cells are expanded in patients with autoimmune and infectious diseases; however their role the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood cell subsets, we observed an inflated DN population variety active inflammatory conditions: myasthenia gravis, Guillain-Barré syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, rheumatic disorders. Furthermore, were able...
Introduction B cells are acknowledged as crucial players in the pathogenesis of multiple sclerosis (MS). Several disease modifying drugs including cladribine have been shown to exert differential effects on peripheral blood cell subsets. However, little is known regarding functional changes within populations. In this study, we obtained a detailed picture repertoire under treatment combined immunoglobulin (Ig) transcriptome and proteome level. Methods We performed next-generation sequencing...
To investigate if patients with neuromyelitis optica spectrum disorder (NMOSD) develop subclinical visual pathway impairment independent of acute attacks.A total 548 longitudinally assessed full-field evoked potentials (VEP) 167 NMOSD from 16 centers were retrospectively evaluated for changes P100 latencies and P100-N140 amplitudes. Rates change in (RCL) amplitudes (RCA) over time analyzed each individual eye using linear regression compared generalized estimating equation models.The rates...
B cells are fundamental players in the pathophysiology of autoimmune diseases central nervous system, such as multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD). A deeper understanding disease-specific cell functions has led to differentiation both development different treatment strategies. While NMOSD is strongly associated with pathogenic anti-AQP4 IgG antibodies proinflammatory cytokine pathways, no valid autoantibodies have been identified MS yet, apart from...
Background Several disease modifying drugs (DMDs) have been approved for the treatment of multiple sclerosis (MS), however, little is known about their differential impact on peripheral blood (PB) B cell subsets. Methods We performed a cross sectional study PB cells in MS patients treated with interferon-β (n = 25), glatiramer acetate 19), dimethyl fumarate 15), fingolimod 16) or natalizumab 22), untreated 20), and non-inflammatory neurological diseases 12). Besides analyzing routine...
Abstract Introduction In Multiple Sclerosis (MS), patients´ characteristics and (bio)markers that reliably predict the individual disease prognosis at onset are lacking. Cohort studies allow a close follow-up of MS histories thorough phenotyping patients. Therefore, multicenter cohort study was initiated to implement wide spectrum data in newly diagnosed Methods ProVal-MS (Prospective validate multidimensional decision score predicts treatment outcome 24 months untreated patients with...
To assess alterations in the composition of peripheral immune cells acute progressive multifocal leukoencephalopathy (PML).Fresh blood samples from 5 patients with PML and 10 healthy controls were analyzed by flow cytometry for naive, central memory effector CD4 CD8 T cells, B lymphocytes, plasma blasts, natural killer (NK) cells. The frequency was determined longitudinally during course 2 patients.The frequencies NK not altered PML. In contrast, naive (p = 0.04) < 0.00001) reduced increased...
Background Within the past 10 years, immune mechanisms associated with acute ischemic stroke (AIS) have been brought into focus, but data on B cell activation and intrathecal Ig production is still scarce. In this study, we determined prevalence of an elevated IgG index, positive oligoclonal bands (OCBs) chemokine C-X-C motif ligand 13 (CXCL13) levels in cerebrospinal fluid (CSF) as markers synthesis patients AIS. Methods a retrospective study analyzed from 212 AIS December 2013 to May 2018...
A strong association between multiple sclerosis (MS) and Epstein-Barr virus (EBV) has been established but the exact role of EBV in MS remains controversial. Recently, molecular mimicry EBNA1 specific GlialCAM, CRYAB ANO2 peptides suggested as a possible pathophysiological mechanism. The aim this study was to analyse anti-EBV antibodies patients against (I) lifecycle proteins, (II) putative cross-reactive peptides, (III) during treatment.
1 Abstract Double negative (DN) (CD19 + CD20 low CD27 − IgD ) B cells are expanded in patients with autoimmune and infectious diseases; however their role the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood cell subsets, we observed an inflated DN population variety active inflammatory conditions: myasthenia gravis, Guillain-Barré syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, rheumatic disorders. Furthermore,...
The long-term and potential rare side effects of new immunomodulating drugs for the treatment multiple sclerosis (MS) are often not well known. Spontaneous case report systems adverse drug a valuable source in pharmacovigilance, but have several limitations. Primary data collections within registries allow comprehensive analysis effects, face challenges. This article will outline chances challenges registry-based event reporting, using example German immunotherapeutic registry REGIMS. REGIMS...
Relapses in multiple sclerosis (MS) patients are usually defined as subacute clinical symptoms that last for at least 24 h. To validate a relapse on magnetic resonance imaging (MRI), an anatomically fitting lesion with gadolinium enhancement the central nervous system (CNS) would be mandatory. The aim of this study was to relapses regard concomitant detection active, MRI lesions. We performed retrospective analysis 199 MS acute who had received scan before initiation methylprednisolone (MPS)...
All-trans retinoic acid (ATRA) is an derivative of vitamin A which discussed as a promising candidate to ameliorate the disease course multiple sclerosis (MS) by immunomodulation or even promoting regeneration in progressive MS. Here we report patient who significantly improved for MS related disability following administration chemotherapy including ATRA mitoxantrone-related acute promyelocytic leukemia and assess effect high-dose three additional patients with
In patients with slowly progressive spastic paraparesis, the differential diagnosis of primary multiple sclerosis (PPMS) and hereditary paraplegia (HSP) can be challenging. Serum neurofilament light chain (sNfL) glial fibrillary acidic protein (sGFAP) are promising fluid biomarkers to support diagnostic workup. NfL is a marker neuroaxonal decay sensitive temporal changes, while elevated sGFAP levels may reflect astrocytal involvement in PPMS. We assessed sNfL 25 PPMS, SPG4 (the most common...
Abstract BACKGROUND Meningeosis carcinomatosa is a diffuse dissemination of tumor cells in the cerebrospinal fluid (CSF) and/or meninges and occurs around 5% patients with malignant melanoma. In previous studies on CSF immune cell subsets an elevated B fraction was found subset melanoma leptomeningeal spread. Elevated are mostly associated neuro-inflammatory diseases largely absent during physiological conditions. Hence this study aims to assess if these resemble intrathecal tumor-specific...
Abstract COVID-19 vaccines based on a range of expression platforms have shown considerable protective efficacy, generating antibody and T cell immune responses. However, molecular pathways underpinning vaccine priming immunity against the SARS-CoV-2 virus not yet been explored extensively. This analysis is critical to optimization future vaccination strategies, schedules, combinations. Thus, we investigated cohort individuals pre- post-vaccination understand humoral cellular response...