A5048417448- Cancer, Lipids, and Metabolism
- Autoimmune Bullous Skin Diseases
- Immune cells in cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Curcumin's Biomedical Applications
- Urticaria and Related Conditions
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Lipoproteins and Cardiovascular Health
- Immunotherapy and Immune Responses
- Nanoparticles: synthesis and applications
- Bone Tissue Engineering Materials
- Graphene and Nanomaterials Applications
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Cancer Immunotherapy and Biomarkers
- Cancer, Stress, Anesthesia, and Immune Response
- Coccidia and coccidiosis research
- RNA Interference and Gene Delivery
- Bird parasitology and diseases
- Laser-Ablation Synthesis of Nanoparticles
- Chemotherapy-induced cardiotoxicity and mitigation
Babeș-Bolyai University
2014-2024
Iuliu Hațieganu University of Medicine and Pharmacy
2017-2019
University of Freiburg
2010-2013
University Medical Center Freiburg
2012
University of Lübeck
2008-2009
The aim of this work was to use the quality-by-design (QbD) approach in development long-circulating liposomes co-loaded with curcumin (CUR) and doxorubicin (DOX) evaluate cytotoxic potential these vitro using C26 murine colon carcinoma cell line. Based on a risk assessment, six parameters, namely phospholipid, CUR DOX concentrations, phospholipid:cholesterol molar ratio, temperature during evaporation hydration steps pH phosphate buffer, were identified as factors for quality final product....
Tailoring extracellular vesicles (EVs) as targeted drug delivery systems to enhance the therapeutic efficacy showed superior advantage over liposomal therapies. Herein, we developed a novel nanotool for targeting B16.F10 murine melanoma, based on EVs stabilized with Polyethylene glycol (PEG) and loaded doxorubicin (DOX). Small were efficiently enriched from melanoma cells cultured under metabolic stress by ultrafiltration coupled size exclusion chromatography (UF-SEC) characterized size,...
The main objective of our research is to develop tumor-targeted therapies that modulate the tumor microenvironment (TME) enhance therapeutic efficacy while minimizing side effects. Specifically, focus lies in two key strategies: targeting tumor-associated macrophages (TAMs) supress their protumor functions and utilizing extracellular vesicles (EVs) derived from dendritic cells intratumor drug delivery immunotherapy. We have previously explored potential statins, such as simvastatin (SIM),...
Chemoresistance continues to remain a significant barrier the effective treatment of various cancers, including melanoma. Previous studies, our own, have demonstrated limited efficacy doxorubicin (DOX) against melanoma cells and tumors, both in vitro vivo, primarily due acquired chemoresistance. The lack favorable outcomes metastatic patients treated with liposomal DOX highlights need identify early DOX-induced resistance biomarkers develop combination strategies improve efficacy. This study...
Abstract Epidermolysis bullosa acquisita is a prototypical organ-specific autoimmune disease caused by autoantibodies against type VII collagen of the dermal-epidermal junction. Although mechanisms autoantibody-induced blister formation were extensively characterized, initiation autoantibody production in blistering diseases still poorly defined. In current study, we addressed role T cells for blister-inducing mice immunized with collagen. To detect autoreactive collagen-specific cells,...
Quality by design principles (QbD) were used to assist the formulation of prednisolone-loaded long-circulating liposomes (LCL-PLP) in order gain a more comprehensive understanding preparation process. This approach enables us improve final product quality terms liposomal drug concentration, encapsulation efficiency and size, minimize variability. A 19-run D-optimal experimental was study impact highest risk factors on PLP concentration (Y1- μg/ml), (Y2-%) size (Y3-nm). Out six investigated...
IgA represents the most prominent Ab class at mucosal surfaces and second prevalent in human blood after IgG. We recently demonstrated that cross-linking of granulocyte FcR (FcαRI) by induces a chemotactic-driven positive-feedback migration loop, hereby amplifying recruitment granulocytes to deposits. Therefore, we postulated aberrant IgA-Ag complexes, which can be found tissues IgA-mediated diseases, are responsible for tissue damage inducing continuous activation. Using an IgA-dependent...
The goal of the current study was to investigate pharmacokinetic profile, tissue distribution and adverse effects long-circulating liposomes (LCL) with curcumin (CURC) doxorubicin (DOX), in order provide further evidence for previously demonstrated enhanced antitumor efficacy colon cancer models. studies were carried out healthy rats, following i.v. injection a single dose LCL-CURC-DOX (1 mg/kg DOX). For study, DOX concentration tumours, heart liver measured after administration two doses...
Background Epidermolysis bullosa acquisita (EBA) is a severe autoimmune skin disease characterized by tissue-bound and circulating autoantibodies to type VII collagen, the major component of anchoring fibrils. When passively transferred into mice, rabbit IgG against collagen induces Fc-dependent activation complement, recruitment leucocytes skin, subepidermal blistering. In addition these inflammatory mechanisms, clinical experimental evidence suggests that antibodies might induce blisters...
Regardless of recent progress, melanoma is very difficult to treat, mainly due the drug resistance modulated by tumor cells as well microenvironment (TME). Among immune recruited at site, associated macrophages (TAMs) are most abundant, promoting important tumorigenic processes: angiogenesis, inflammation and invasiveness. Furthermore, it has been shown that TAMs involved in mediating cells. Thus, present study, we used liposomal formulation prednisolone disodium phosphate (LCL-PLP) inhibit...
An increasing number of studies published so far have evidenced the benefits Simvastatin (SIM) and Doxorubicin (DOX) co-treatment in colorectal cancer. In view this, current study aimed to investigate pharmaceutical development liposomes co-encapsulating SIM DOX, by implementing Quality Design (QbD) concept, as a means enhance antiproliferative effect co-formulation on C26 murine colon cancer cells co-cultured with macrophages. It is known that quality profile dependent critical attributes...
Primary melanoma aggressiveness is determined by rapid selection and growth of cellular clones resistant to conventional treatments, resulting in metastasis recurrence. In addition, a reprogrammed tumor-immune microenvironment supports progression response therapy. There an urgent need develop selective specific drug delivery strategies for modulating the interaction between cancer cells immune within tumor microenvironment. This study proposes novel combination therapy consisting sequential...
Purpose: Besides cholesterol lowering effects, simvastatin (SIM) at very high doses possesses antitumor actions.Moreover our previous studies demonstrated that tumor-targeted delivery of SIM by using long-circulating liposomes (LCL) improved the therapeutic index this drug in murine melanoma-bearing mice.To evaluate whether finding can be exploited for future therapy colorectal cancer activity and underlying mechanisms liposomal (LCL-SIM) efficacy inhibition C26 colon carcinoma growth vivo...
5-Fluorouracil-based therapy remains the main approach in colorectal cancer, even though there are still some drawbacks, such as chemoresistance. In this study we combined 5-fluorouracil encapsulated long-circulating liposomes with simvastatin, also liposomes, that was previously proved to exert antitumor actions on same tumor model. The production of angiogenic/inflammatory proteins assessed by protein array and markers for aggressiveness (Bcl-2, Bax, nuclear factor [NF]-κB) were determined...
Bullous pemphigoid is an autoimmune blistering skin disease associated with autoantibodies against the dermal-epidermal junction. Passive transfer of antibodies BP180/collagen (C) XVII, a major hemidesmosomal antigen, into neonatal mice results in separation upon applying gentle pressure to their skin, but not spontaneous blistering. In addition, this mouse model precludes treatment and observation diseased animals beyond 2-3 days. Therefore, present study we have developed new reproducing...
Colorectal cancer remains one of the major causes morbidity and mortality in both developed emerging countries. Cancer stem cells (CSCs) are a subpopulation within tumor mass harboring cell characteristics, considered responsible for initiation, growth, relapse, treatment failure. Lately, it has become clear that CSCs non-CSCs have to be eliminated successful eradication cancer. Drug delivery systems been extensively employed enhance drug efficacy. In this study, salinomycin (SAL), selective...
Backround:Ajuga species have been used in traditional medicine for their diuretic, anti-inflammatory, wound-healing, and hepatoprotective properties. Purpose: The phytochemical profile anticancer potential of three Ajuga sp. (A. genevensis, A. chamaepitys, laxmannii) from Romania was investigated. Materials Methods: phytochemicals were extracted the aerial parts by using different solvents methods. hydroalcoholic extracts examined total phenolic, flavonoid iridoid contents, HPLC/MS to...
Previous studies have demonstrated that tumor-associated macrophages (TAMs) are pivotal players in tumor progression via modulation of angiogenesis, inflammation, metastasis and oxidative stress, as well the response cancer cells to cytotoxic drugs. Nevertheless, role TAMs prognosis colorectal remains controversial. Therefore, present study aimed investigate how mediate C26 colon carcinoma drug 5-fluorouracil (5-FU), upon TAM co-cultivation with these vitro. In this respect, 5-FU...