A5067079294- Inflammatory mediators and NSAID effects
- Antiplatelet Therapy and Cardiovascular Diseases
- Eicosanoids and Hypertension Pharmacology
- Synthesis of β-Lactam Compounds
- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Venous Thromboembolism Diagnosis and Management
- Cancer, Lipids, and Metabolism
- Hormonal Regulation and Hypertension
- Inflammatory Biomarkers in Disease Prognosis
- Neuropeptides and Animal Physiology
- Antioxidant Activity and Oxidative Stress
- Analytical Methods in Pharmaceuticals
- Helicobacter pylori-related gastroenterology studies
- Cancer-related molecular mechanisms research
- Lipoproteins and Cardiovascular Health
- Blood Pressure and Hypertension Studies
- Extracellular vesicles in disease
- Free Radicals and Antioxidants
- Platelet Disorders and Treatments
- Drug-Induced Adverse Reactions
- Peroxisome Proliferator-Activated Receptors
- Alcohol Consumption and Health Effects
- Adenosine and Purinergic Signaling
- Gastroesophageal reflux and treatments
University of Chieti-Pescara
2015-2025
University of Pennsylvania
2006-2019
Translational Therapeutics (United States)
2006-2019
Shanghai Institutes for Biological Sciences
2019
Chinese Academy of Sciences
2019
Tianjin Medical University
2019
Catholic University of America
2000-2019
Institute on Aging
2012
Ospedale SS. Annunziata
2004-2011
New York Medical College
2008
The current controversy on the potential cardioprotective effect of naproxen prompted us to evaluate extent and duration platelet, monocyte, vascular cyclooxygenase (COX) inhibition by compared with low-dose aspirin.We performed a crossover, open-label study aspirin (100 mg/d) or (500 mg BID) administered 9 healthy subjects for 6 days. effects thromboxane (TX) prostacyclin biosynthesis were assessed up 24 hours after oral dosing. Serum TXB2, plasma prostaglandin (PG) E2, urinary...
Hypertensive patients with renovascular disease (RVD) may be exposed to increased oxidative stress, possibly related activation of the renin-angiotensin system.We measured urinary excretion 8-iso-prostaglandin (PG) F2alpha and 11-dehydro-thromboxane (TX) B2 as indexes in vivo lipid peroxidation platelet activation, respectively, 25 RVD, essential hypertension, healthy subjects. Plasma renin activity peripheral renal veins, angiotensin II cholesterol, glucose, triglycerides, homocysteine,...
Diabetes mellitus is associated with platelet hyperactivity, which leads to increased morbidity and mortality from cardiovascular disease. This coupled enhanced levels of thromboxane (TX), an eicosanoid that facilitates aggregation. Although intensely studied, the mechanism underlying relationship among hyperglycemia, TX generation, hyperactivity remains unclear. We sought identify key signaling components connect high glucose generation examine their clinical relevance. In human platelets,...
Aspirin affords cardioprotection through the acetylation of serine 529 in human cyclooxygenase-1 (COX-1) anucleated platelets, inducing a permanent defect thromboxane A 2 (TXA )–dependent platelet function. However, heterogeneity COX-1 suppression by aspirin has been detected cardiovascular disease and may contribute to failure prevent clinical events. The recent recognized capacity platelets make proteins de novo paves way identify new mechanisms involved variable response aspirin. We found...
We performed a placebo-controlled, randomized study to address whether celecoxib or ibuprofen undermines the functional range of inhibition platelet cyclooxygenase (COX)-1 activity by aspirin in patients with osteoarthritis and stable ischemic heart disease.Twenty-four who were undergoing long-term treatment (100 mg daily) for cardioprotection coadministered celecoxib, 200 twice daily, ibuprofen, 600 3 times placebo 7 days.The coadministration did not undermine aspirin-related COX-1...
3-(5-Chlorofuran-2-yl)-5-methyl-4-phenylisoxazole (P6), a known selective cyclooxygenase-1 (COX-1) inhibitor, was used to design new series of 3,4-diarylisoxazoles in order improve its biochemical COX-1 selectivity and antiplatelet efficacy. Structure-activity relationships were studied using human whole blood assays for COX-2 inhibition vitro, results showed that the simultaneous presence 5-methyl (or -CF3), 4-phenyl, 5-chloro(-bromo or -methyl)furan-2-yl groups on isoxazole core essential...
Recent findings have shown that aspirin, taken for several years, reduces the long-term risk of some cancers, particularly colorectal cancer. The result aspirin benefit is detectable at daily low-doses (at least 75mg), same used prevention cardiovascular disease, positions antiplatelet action center its antitumor efficacy. At given every 24 h, acting by a complete and persistent inhibition cyclooxygenase (COX)-1 in platelets (in pre-systemic circulation) while causing limited rapidly...
Platelet-cancer cell interactions modulate tumor metastasis and thrombosis in cancer. Platelet-derived extracellular vesicles (EVs) can contribute to these outcomes.We characterized the medium-sized EVs (mEVs) released by thrombin-stimulated platelets of colorectal cancer (CRC) patients healthy subjects (HS) on capacity induce epithelial-mesenchymal transition (EMT)-related genes cyclooxygenase (COX)-2(PTGS2), thromboxane (TX)B2 production cocultures with four lines. mEVs were assessed for...
Background —Increased formation of 8-iso-prostaglandin (PG) F 2α and thromboxane (TX) A 2 , potent agonists platelet vascular (TH)/PGH receptors, has been detected in cigarette smokers. We performed a randomized, double-blind, placebo-controlled study the effects vitamin E (300, 600, 1200 mg/d, each dose for 3 consecutive weeks) on 8-iso-PGF TXA biosynthesis 46 moderate Methods Results —Urinary immunoreactive 11-dehydro-TXB plasma E, serum TXB were measured by previously validated...
The aim of the present cross-sectional study was to investigate whether activation renin-angiotensin system in renovascular disease affects cytochrome P450 omega/omega-1 hydroxylase (20-hydroxyeicosatetraenoic acid [20-HETE]) and epoxygenase (epoxyeicosatrienoic acids [EETs]) pathways arachidonic metabolism vivo, each which interacts with angiotensin II. Plasma concentration urinary excretion 20-HETE EETs their metabolites, dihydroxyeicosatrienoic acids, were measured urine plasma by mass...
We compared the variability in degree and recovery from steady-state inhibition of cyclooxygenase (COX)-1 COX-2 ex vivo platelet aggregation by naproxen sodium at 220 versus 440 mg b.i.d. low-dose aspirin healthy subjects. Six subjects received consecutively (220 b.i.d.) (100 daily) for 6 days, separated washout periods 2 weeks. COX-1 was determined using indices enzymatic activity: 1) measurement serum thromboxane (TX)B<sub>2</sub> levels whole-blood lipopolysaccharide-stimulated...
Background.Inflammation, oxidative damage, and platelet activation are hypothesized biological mechanisms driving the disablement process. The aim of present study is to assess whether biomarkers representing these predicted major adverse health-related events in older persons.