A5046543021- Blood transfusion and management
- Blood groups and transfusion
- Blood donation and transfusion practices
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Hematopoietic Stem Cell Transplantation
- Platelet Disorders and Treatments
- Hemoglobin structure and function
- Hepatitis B Virus Studies
- Complement system in diseases
- Immune Cell Function and Interaction
- Erythropoietin and Anemia Treatment
- CAR-T cell therapy research
- Neurological Complications and Syndromes
- Organ Donation and Transplantation
- Virus-based gene therapy research
- Retinal and Optic Conditions
- Iron Metabolism and Disorders
- Parvovirus B19 Infection Studies
- Neonatal Health and Biochemistry
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- HIV Research and Treatment
- Sepsis Diagnosis and Treatment
- Mesenchymal stem cell research
National Institutes of Health Clinical Center
2015-2025
National Institutes of Health
2012-2024
Hadassah Medical Center
2021
Seattle University
2016-2020
Hebrew University of Jerusalem
2020
Emerald Coast Science Center
2018
John Wiley & Sons (United States)
2016
Iowa City Public Library
2016
Hudson Institute
2016
Naval Medical Center San Diego
2012
In 1990, a clinical trial was started using retroviral-mediated transfer of the adenosine deaminase (ADA) gene into T cells two children with severe combined immunodeficiency (ADA − SCID). The number blood normalized as did many cellular and humoral immune responses. Gene treatment ended after 2 years, but integrated vector ADA expression in persisted. Although components remain to be perfected, it is concluded here that therapy can safe effective addition for some patients this disease.
Two monoclonal antibodies (CLB-CD 27/1 and CLB-CD 27/2) were raised against a novel determinant on human T lymphocytes. One of these antibodies, (clone 9F4), was grouped by the Third International Workshop Conference Human Leucocyte Differentiation Antigens together with three other (VIT 14, OKT 18A, S152) in new cluster CD27. In this paper we show that belonging to recognize an antigen present large subset peripheral lymphocytes most medullary thymocytes. At least two different...
Interleukin-2 (IL-2) has direct pluripotent effects on cells with immune and inflammatory function. Which of these a critical role in mediating tumor regression remains enigmatic. In this study, we compared early changes transcriptional profiles circulating mononuclear those occurring within the microenvironment melanoma metastases following systemic IL-2 administration.The results suggest that immediate administration is activation genes predominantly associated monocyte cell function;...
Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components millimolar concentration. Intracellular ascorbic depleted greater than 96% during cell culture rapidly reaccumulated after addition of physiologic concentrations to the extracellular medium. Purified cells maintained concentration gradients as large 100-fold across plasma membrane. The ability vary intracellular over such a wide range makes it possible for first time these study...
Clinical trial designs that randomize patients to fixed treatment regimens may disrupt preexisting relationships between illness severity and level of therapy. The practice misalignments created by such have unintended effects on results safety.To illustrate this problem, the Transfusion Requirements in Critical Care (TRICC) Acute Respiratory Distress Syndrome Network low tidal volume (ARMA) were analyzed.Publications before TRICC indicated clinicians used higher transfusion thresholds with...
Two patients receiving intravenous heparin for pulmonary emboli developed disseminated intravascular coagulation during therapy. Both were adequate anticoagulation before the onset of this syndrome. Mechanisms that might cause or prolong therapy are proposed.
Pooling multiple swab samples before RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis has been proposed as a strategy to reduce costs increase throughput of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests. However, reports on practical large-scale group testing for SARS-CoV-2 have scant. Key open questions concern reduced sensitivity due sample dilution, the rate false positives, actual efficiency (number tests saved by...
We have applied the technique of laser-Doppler velocimetry to compare patterns cutaneous blood flow in forearms patients with stable sickle-cell disease, normal subjects matched for age, race, and sex, anemia due beta+-thalassemia. The mean resting disease was comparable that control groups but associated large, local oscillations periods 7 10 seconds peak-to-trough magnitudes about half flow. Oscillations occurred simultaneously at sites separated by 1 cm were independent phase frequency....
We performed a multicenter study in 1989 to determine whether screening whole-blood donors for human immunodeficiency virus type 1 (HIV-1) p24 antigen would improve transfusion safety by identifying carriers of the who are seronegative HIV-1 antibody.
A retrospective study was undertaken to determine the frequency of alloimmunization in two multitransfused patient populations. Three hundred eighteen unselected patients whose transfusion records were available studied. One fifty-seven had sickle cell disease (SCD) and 161 thalassemia. Forty-four antibodies detected 23 99 transfused SCD patients. The predominate Rh group: seventeen anti-rh"(E), eight anti-rh'(C), four anti-rh'w(Cw). Of remaining antibodies, five anti-Kell, three anti-Jka,...
A retrospective study was performed to examine the natural course of uncontrolled thrombocytosis associated with chronic myeloproliferative disorders. Thirty-eight patients polycythaemia rubra vera (PV), myelofibrosis/myeloid metaplasia (MM), myelogenous leukaemia (CML) or essential thrombocythaemia (ET) had platelet counts greater than 1000 X 10(9)/1 and were followed closely for a total 246 patient years. Eleven experienced haemorrhagic episodes. Bleeding twice as frequent in over 59 years...