A5038112028- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and biosynthesis
- Cardiovascular Disease and Adiposity
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Pancreatic function and diabetes
- Bariatric Surgery and Outcomes
- Metabolism, Diabetes, and Cancer
- Nutrition and Health in Aging
- Peroxisome Proliferator-Activated Receptors
- Regulation of Appetite and Obesity
- Endoplasmic Reticulum Stress and Disease
- Diabetes and associated disorders
- RNA modifications and cancer
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- Muscle metabolism and nutrition
- Body Contouring and Surgery
- Nerve injury and regeneration
- RNA Research and Splicing
- Signaling Pathways in Disease
- Mesenchymal stem cell research
- Birth, Development, and Health
- Diabetes Treatment and Management
Karolinska University Hospital
2016-2025
Karolinska Institutet
2016-2025
Steno Diabetes Centers
2024-2025
Linköping University Hospital
2024
Landstinget i Kalmar län
2024
Linköping University
2010-2012
Inserm
2009
Université de Montpellier
2009
Humboldt-Universität zu Berlin
2008
Stockholm University
2008
Glial cell line-derived neurotrophic factor (GDNF) is a polypeptide, distantly related to transforming growth factor-beta (TGF-beta), originally isolated by virtue of its ability induce dopamine uptake and survival in cultures embryonic ventral midbrain dopaminergic neurons, more recently shown be potent for motorneurons. The biological activities distribution this molecule outside the central nervous system are presently unknown. We report here on mRNA expression, initial receptor binding...
OBJECTIVE Adipose tissue may contain few large adipocytes (hypertrophy) or many small (hyperplasia). We investigated factors of putative importance for adipose morphology. RESEARCH DESIGN AND METHODS Subcutaneous adipocyte size and total fat mass were compared in 764 subjects with BMI 18–60 kg/m2. A morphology value was defined as the difference between measured volume expected given by a curved-line fit body related to insulin values. In 35 subjects, vivo turnover exploiting incorporation...
Comprehensive proteomic profiling of the human adipocyte secretome identified dipeptidyl peptidase 4 (DPP4) as a novel adipokine. This study assessed functional implications adipokine DPP4 and its association to metabolic syndrome.Human adipocytes skeletal smooth muscle cells were used monitor release assess effects soluble on insulin signaling. In lean obese subjects, depot-specific expression from adipose tissue explants determined correlated parameters syndrome.Fully differentiated...
The mobilization of fat stored in adipose tissue is mediated by hormone-sensitive lipase (HSL) and the recently characterized triglyceride (ATGL), yet their relative importance lipolysis unknown. We show that a novel potent inhibitor HSL does not inhibit other lipases. compound counteracted catecholamine-stimulated mouse adipocytes had no effect on residual hydrolysis HSL-null mice. In human adipocytes, catecholamine- natriuretic peptide–induced were completely blunted inhibitor. When cells...
In obesity, white adipose tissue (WAT) inflammation is linked to insulin resistance. Increased adipocyte chemokine (C-C motif) ligand 2 (CCL2) secretion may initiate by attracting the migration of inflammatory cells into tissue. Using an unbiased approach, we identified microRNAs (miRNAs) that are dysregulated in human obesity and assessed their possible role controlling CCL2 production. subcutaneous WAT obtained from 56 subjects, 11 miRNAs were present all subjects downregulated obesity. Of...
The contribution of cellular heterogeneity and architecture to white adipose tissue (WAT) function is poorly understood. Herein, we combined spatially resolved transcriptional profiling with single-cell RNA sequencing image analyses map human WAT composition structure. This identified 18 cell classes unique propensities form organized homo- heterotypic clusters. Of these, three constituted mature adipocytes that were similar in size, but distinct their spatial arrangements profiles. Based on...
Abstract Mitochondrial dysfunction is a characteristic trait of human and rodent obesity, insulin resistance fatty liver disease. Here we show that high-fat diet (HFD) feeding causes mitochondrial fragmentation in inguinal white adipocytes from male mice, leading to reduced oxidative capacity by process dependent on the small GTPase RalA. RalA expression activity are increased after HFD. Targeted deletion prevents mitochondria diminishes HFD-induced weight gain increasing acid oxidation....
Abstract Reducing body weight to improve metabolic health and related comorbidities is a primary goal in treating obesity 1,2 . However, maintaining loss considerable challenge, especially as the seems retain an obesogenic memory that defends against changes 3,4 Overcoming this barrier for long-term treatment success difficult because molecular mechanisms underpinning phenomenon remain largely unknown. Here, by using single-nucleus RNA sequencing, we show both human mouse adipose tissues...
Loss of fat mass is a key feature cancer cachexia and has been attributed to increased adipocyte lipolysis. The mechanism behind this alteration unknown was presently investigated. We studied mature s.c. cells differentiated preadipocytes from 26 patients with without cachexia. Hormone-induced lipolysis expression lipolysis-regulating genes were determined together body composition in vivo lipolytic activity (fasting plasma glycerol or fatty acids related fat). Body reduced by 40% 2-fold (P...
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine with proposed role in obesity-related insulin resistance. This could be mediated by increased lipolysis adipose tissue resulting elevated free fatty acid levels. The early intracellular signals entailed TNF-α-mediated are unknown but may involve members of the mitogen-activated protein kinase (MAPK) family. We investigated possible contribution MAPK TNF-α-induced human preadipocytes. TNF-α activated three mammalian MAPK, p44/42, JNK,...
Fibroblast growth factor 21 (FGF21) is active in murine adipocytes and has beneficial metabolic effects animal models of type 2 diabetes mellitus. We assessed whether FGF21 influences lipolysis human 3T3‐L1 cells. had no short‐time effect (h) while a 3‐day incubation with attenuated hormone‐stimulated lipolysis. did not influence the mRNA expression genes involved regulating lipolysis, but significantly reduced lipid droplet‐associated phosphoprotein perilipin without affecting...
Lipolysis is the catabolic pathway by which triglycerides are hydrolyzed into fatty acids. Adipose triglyceride lipase (ATGL) and hormone-sensitive (HSL) have capacity to hydrolyze in vitro first ester bond of triglycerides, but their respective contributions whole cell lipolysis human adipocytes unclear. Here, we investigated roles HSL, ATGL, its coactivator CGI-58 basal forskolin-stimulated a white adipocyte model, hMADS cells. The express various components acid metabolism show lipolytic...
Site differences in adipose tissue function may have implications for insulin-resistant conditions. In mature tissue, subcutaneous adipocytes higher leptin secretion, similar tumor necrosis factor (TNF)-α and lower catecholamine-stimulated lipolysis as compared with omental adipocytes. this study, TNF-α secretion were between human preadipocytes. After 16 days of incubation a minimal differentiation medium, mRNA found to be two eight times than preadipocytes (P < 0.05). On the other...
Elevated circulating fatty acid concentration is a hallmark of insulin resistance and at least in part attributed to the action adipose tissue-derived tumor necrosis factor-α (TNF-α) on lipolysis. Cell death-inducing DFFA (DNA fragmentation factor-α)-like effector A (CIDEA) belongs family proapoptotic proteins that has five known members humans mice. The CIDEA unknown, but CIDEA-null mice are resistant obesity diabetes. We investigated tissue obese lean mRNA was expressed white human fat...
Abstract BACKGROUND. Cancer cachexia is an important, negative prognostic marker that has been linked to systemic inflammation and cell death through unclear mechanisms. A key feature of cancer loss white adipose tissue (WAT) because increased adipocyte lipolysis possibly reduced lipid synthesis (lipogenesis). In this study, the authors investigated whether alterations in fat numbers, lipogenesis, or cytokine and/or leukocyte infiltration could account for some functional changes observed...
Although elevated free fatty acid (FFA) levels in obesity have been considered to be of importance for insulin resistance, a recent meta-analysis suggested normal FFA obese subjects. We investigated fasting circulating and glycerol large cohort non-obese subjects.Subjects recruited study on genetics were the morning after an overnight fast (n = 3,888). Serum 3,306), plasma 3,776), sensitivity index (HOMA-IR,n 3,469) determined. Obesity was defined as BMI ≥ 30 kg/m 2 resistance HOMA-IR...