A5021773468- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS Research and Interventions
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Influenza Virus Research Studies
- Chemical Synthesis and Analysis
- Developmental Biology and Gene Regulation
- vaccines and immunoinformatics approaches
- Neurobiology and Insect Physiology Research
- Hemophilia Treatment and Research
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Blood groups and transfusion
Georg Speyer Haus
2013-2024
Goethe University Frankfurt
2006-2014
University of Siena
2010
Centre National de la Recherche Scientifique
2006
Bayer (Germany)
1997-1999
Hessian Ministry for Science and the Arts
1999
Tel Aviv University
1997
Israel Water Authority
1997
Kaplan Medical Center
1997
Hadassah Academic College
1997
Abstract The neurogenic genes of Drosophila melanogaster are involved in the decision ectodermal cells to take on a neural or an epidermal fate. We present evidence support notion that six functionally related. studied phenotype embryos lacking one presence increased dosage wild-type allele another gene. Our analysis also included Hairless locus, whose function is related genes, as well many other genes. effects observed were asymmetric triploidy for given gene modified loss gene, but latter...
As cancer treatment tools, oncolytic viruses (OV) have yet to realize what some see as their ultimate clinical potential. In this study, we engineered a chimeric vesicular stomatitis virus (VSV) that is devoid of its natural neurotoxicity while retaining potent activity. The envelope glycoprotein (G) VSV was replaced with variant the lymphocytic choriomeningitis (LCMV-GP), creating replicating therapeutic, rVSV(GP), benign in normal brain but can effectively eliminate multiple preclinical...
DNA sequences encoding the surface envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and 2 (HIV-2) were amplified by PCR from uncultured peripheral blood mononuclear cells obtained patients with serologically defined HIV-1/HIV-2 mixed infections Bombay, India. HIV-1-specific products in seven randomly chosen doubly reactive cases, while HIV-2-specific detected five cases (71%). sequence analysis showed that HIV-1 gp120 coding closely related to each other (nucleotide...
Six zygotically expressed genomic loci of Drosophila melanogaster, N, bib, mam, neu, Dl and E (spl), have previously been found to be involved in the commitment ectodermal cells as neuroblasts. We studied phenotypes induced by various mutant alleles at these 6 imaginal epidermal cells, order assess possible implications genes functions other than early neurogenesis. When homozygous, any mutations except bib affected development bristles compound eye ways. These range from cell death...
ABSTRACT Vesicular stomatitis virus (VSV)-based oncolytic virotherapy has the potential to significantly improve prognosis of aggressive malignancies such as brain cancer. However, VSV's inherent neurotoxicity hindered clinical development so far. Given that this neurotropism is attributed glycoprotein VSV-G, VSV was pseudotyped with nonneurotropic envelope lymphocytic choriomeningitis (LCMV-GP→VSV-GP). Compared VSV, VSV-GP showed enhanced infectivity for cancer cells in vitro while sparing...
DEAD-box proteins are enzymes endowed with nucleic acid-dependent ATPase, RNA translocase and unwinding activities. The human protein DDX3 has been shown to play important roles in tumor proliferation viral infections. In particular, identified as an essential cofactor for HIV-1 replication. Here we characterized a set of mutants biochemically respect acid binding, ATPase helicase activity. addressed the functional role unique insertion between motifs I Ia provide evidence its implication...
Objective: To gain molecular insights into different HIV-1 strains present in two states of India, nucleotide sequences derived from the env region four were analysed. Design: was isolated high-risk patients Maharashtra (city Bombay) and Goa. The analysis encompassed all variable domains external glycoprotein, gp120. Methods: Genomic DNA cultured cells infected with each Indian independently amplified by polymerase chain reaction (PCR). PCR fragments cloned sequenced a phylogenetic tree...
V3 serotyping refers to a system based on binding of antibody in patient sera V3-loop peptides derived from HIV-1 env genetic subtypes. The V3X serotype represents reactivity serum an HIV-1-infected (regardless viral subtype), which reacts preferentially peptide the X subtype sequence. We have classified serotypes, determined relationship between serotypes and subtypes large study (n = 125), evaluated performance three different peptide-binding assays. Seven were identified: A, B, B-Br,...
Abstract Neutralizing antibodies often recognize conformational, discontinuous epitopes. Linear peptides mimicking such conformational epitopes can be selected from phage display peptide libraries by screening with the respective antibodies. However, it is difficult to localize these “mimotopes” within three‐dimensional (3D) structures of target proteins. Knowledge neutralizing would help design antigens able elicit protective immune responses. Therefore, we provide here a software that...
We investigated whether V3-binding assays might be useful to analyze human immunodeficiency virus type 1 (HIV-1) variants in different geographic regions. showed that strong cross-reactivity between subtype-specific V3 peptides is almost inevitable standard indirect enzyme-linked immunosorbent (EIA), impairing precise serological subtyping. therefore developed a EIA (HIV-1 SSEIA) uses the principle of blocking by an excess peptide liquid phase. Using 231 serum samples collected from...
Human immunodeficiency virus type 2 (HIV-2)-related viruses were isolated from a Gambian dying of exclusively neurological disease (HIV-2D194) and an asymptomatic Ghanian (HIV-2D205). Both strains exhibited properties HIV-1 biological subtype c: they grew slowly induced few or no syncytia but eventually produced high levels particle-associated reverse transcriptase in cultures fresh peripheral blood lymphocytes, established stable infection T-lymphoma (HUT-78) monocytic (U937) cell lines....
The interaction between the human immunodeficiency virus (HIV) integrase (IN) and its cellular cofactor lens epithelium-derived growth factor (LEDGF/p75) is crucial for HIV replication. While recently discovered LEDGINs inhibit HIV-1 replication by occupying LEDGF/p75 pocket in IN, it remained to be demonstrated whether itself can targeted. By phage display we identified cyclic peptides (CPs) as first ligands that LEDGF/p75–IN interaction. CPs different cell lines without overt toxicity. In...
Abstract Newly emerging influenza A viruses (IAV) pose a major threat to human health by causing seasonal epidemics and/or pandemics, the latter often facilitated lack of pre-existing immunity in general population. Early recognition candidate pandemic (CPIV) is crucial importance for restricting virus transmission and developing appropriate therapeutic prophylactic strategies including effective vaccines. Often, potential newly IAV only fully recognized once starts spread efficiently...