A5014041622- Extracellular vesicles in disease
- Magnetic and Electromagnetic Effects
- MicroRNA in disease regulation
- Cardiovascular Disease and Adiposity
- Microbial Inactivation Methods
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Melanoma and MAPK Pathways
- Microfluidic and Bio-sensing Technologies
- Computational Drug Discovery Methods
- Bacterial Infections and Vaccines
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Immunology Research
- Cardiac Fibrosis and Remodeling
- Phagocytosis and Immune Regulation
- RNA Research and Splicing
- Plant Genetic and Mutation Studies
- Atherosclerosis and Cardiovascular Diseases
- interferon and immune responses
- Gut microbiota and health
Northern California Institute for Research and Education
2021-2024
Yale University
2024
University College Cork
2024
San Francisco VA Medical Center
2022-2024
University of California, San Francisco
2021-2023
Abstract The intestinal microbiota influences mammalian host physiology in health and disease locally the gut but also organs devoid of direct contact with bacteria such as liver brain. Extracellular vesicles (EVs) or outer membrane (OMVs) released by microbes are increasingly recognized for their potential role biological shuttle systems inter‐kingdom communication. However, physiologically relevant evidence transfer functional biomolecules from to individual cells OMVs vivo is scarce. By...
Cardiometabolic disease is an increasing cause of morbidity and death in society. While M1-like macrophages contribute to metabolic inflammation insulin resistance, those polarized M2-like phenotype exert protective properties. Building on our observations reporting macrophage exosomes atherosclerosis control, we tested whether they could serve control the liver adipose tissue obese mice. In thinking clinical translation, studied human THP-1 exposed interleukin (IL)-4 as a source...
Abstract While apolipoprotein E (apoE) expression by myeloid cells is recognized to control inflammation, whether such benefits can be communicated via extracellular vesicles not known. Through the study of produced macrophages derived from bone marrow Wildtype (WT‐BMDM‐EV) and ApoE deficient (EKO‐BMDM‐EV) mice, we uncovered a critical role for apoE in regulating their cell signaling properties. WT‐BMDM‐EV anti‐inflammatory properties recipient increasing cellular levels miR‐146a‐5p, that...
Exosomes are small extracellular vesicles (sEVs) of ~30-150 nm in diameter that enriched exosome marker proteins and play important roles health disease. To address large unanswered questions regarding biology
ABSTRACT Exosomes are small extracellular vesicles (sEVs) of ∼30-150 nm in diameter that have the same topology as cell, enriched selected exosome cargo proteins, and play important roles health disease. To address large unanswered questions regarding biology vivo , we created exomap1 transgenic mouse model. In response to Cre recombinase, mice express HsCD81mNG, a fusion protein between human CD81, most highly yet described, bright green fluorescent mNeonGreen. As expected, cell...
Apolipoprotein E (ApoE) is recognized for its pleiotropic properties that suppress inflammation. We report ApoE serves as a metabolic rheostat regulates microRNA control of glycolytic and mitochondrial activity in myeloid cells hematopoietic stem progenitor (HSPCs). expression increases microRNA-146a, which reduces nuclear factor κB (NF-κB)-driven GLUT1 activity. In contrast, microRNA-142a, carnitine palmitoyltransferase 1a (CPT1A) expression, fatty acid oxidation, oxidative phosphorylation....
Abstract Irreversible electroporation (IRE) is a non-thermal tissue ablative technology that has emerging applications in surgical oncology and regenerative surgery. To advance its therapeutic usefulness, it important to understand the mechanisms through which IRE induces cell death role of innate immune system mediating subsequent repair. Through intravital imaging liver mice, we show produces distinctive injury features, including delayed yet robust recruitment neutrophils, consistent with...
Melanoma is one of the most prevalent cancers in United States, accounting for 5% all new cancer cases, and has been increasing worldwide. 1Melanoma arises from malignant proliferation melanocytes, cells that produce melanin provides skin pigmentation.The high metastatic potential melanoma drastically affects prognosis patients diagnosed at later stages.In particular, 50%-75% cases metastasize to brain, 54% melanoma-related deaths. 2,3It thus critical diagnose earlier stages limit metastasis...
Introduction: Extracellular vesicles (EVs) produced by IL4-polarized macrophages (Mφ) suppress inflammation communicating microRNA-controlled immunometabolic signaling to recipient immune cells and adipocytes. However, detailed cellular molecular pathways responsible for these effects remain incompletely understood. Objectives: Examine transcriptional responses sensitive Mφ EVs among circulating Ly6C hi monocytes derived from mice with hyperlipidemia. Methods/Approach: were culturing THP1...
Abstract Effective treatment strategies to alleviate heart failure that develops as a consequence of myocardial infarction (MI) remain an unmet need in cardiovascular medicine. In this study, we uncovered exosomes produced by human THP-1 macrophages cultured with the cytokine IL-4 (THP1-IL4-exo), reverse cardiac functional decline mice develop MI diet-induced occlusive coronary atherosclerosis. Therapeutic benefits THP1-IL4-exo stem from their ability reprogram circulating Ly-6C hi monocytes...
Irreversible electroporation (IRE) is a tissue ablation technology that kills cells with short electrical pulses do not induce thermal damage, thereby preserving the extracellular matrix. Preclinical research suggests IRE may be developed as tool for regenerative surgery by clearing existing host within solid organ and creating supportive niche new cell engraftment. We hypothesized hepatocytes transplanted injection into portal circulation would preferentially engraft liver parenchyma pretreated IRE.