A5011496603- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cardiac Fibrosis and Remodeling
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Ion channel regulation and function
- Muscle Physiology and Disorders
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Function and Risk Factors
- Fuel Cells and Related Materials
- Cardiovascular Effects of Exercise
- Signaling Pathways in Disease
- Erythrocyte Function and Pathophysiology
- Sarcoidosis and Beryllium Toxicity Research
- Takotsubo Cardiomyopathy and Associated Phenomena
- Nuclear Receptors and Signaling
Massachusetts General Hospital
2021-2024
Harvard University
2021-2024
Sanford Consortium for Regenerative Medicine
2024
University of California, San Diego
2024
Scintillon Institute
2024
Vanderbilt University Medical Center
2024
University of Calcutta
2015-2019
Extracellular vesicles (EVs) are membrane-bound nanoparticles with different types of cargo released by cells and postulated to mediate functions such as intercellular communications. Recent studies have shown that long non-coding RNAs (lncRNAs) or their fragments present within EVs. LncRNAs a heterogeneous group RNA species length exceeding 200 nucleotides diverse in based on localization. While lncRNAs known for important cellular regulation, presence role EVs only recently been explored....
Cardiac hypertrophy and associated heart fibrosis remain a major cause of death worldwide. Phytochemicals have gained attention as alternative therapeutics for managing cardiovascular diseases. These include the extract from plant Terminalia arjuna, which is popular cardioprotectant may prevent or slow progression pathological to failure. Here, we investigated mode action principal bioactive T. arjuna compound, arjunolic acid (AA), in ameliorating hemodynamic load-induced cardiac identified...
BACKGROUNDCardiorenal syndrome (CRS) - renal injury during heart failure (HF) is linked to high morbidity. Whether circulating extracellular vesicles (EVs) and their RNA cargo directly impact its pathogenesis remains unclear.METHODSWe investigated the role of EVs from patients with CRS on epithelial/endothelial cells using a microfluidic kidney-on-chip (KOC) model. The small was regressed against serum creatinine prioritize subsets functionally relevant EV-miRNAs mRNA targets in silico...
Exosomes are small extracellular vesicles (sEVs) of ~30-150 nm in diameter that enriched exosome marker proteins and play important roles health disease. To address large unanswered questions regarding biology
Cell chirality is extremely important for the evolution of cell morphogenesis to manipulate performance due left-right asymmetry. Although chiral micro- and nanoscale biomaterials have been developed regulate functions, how affects nanomechanics command nuclear mechanotransduction was ambiguous. In this study, engineered microcircle arrays were prepared by photosensitive cross-linking synthesis on culture plates control clockwise/counterclockwise geometric topology stem cells. Asymmetric...
Antibodies are critical tools for research into extracellular vesicles (EVs) and other nanoparticles (ENPs), where they can be used their identification, characterization, isolation. However, the lack of a centralized antibody platform researchers share validation results thus minimizing wasted personnel time reagents, has been significant obstacle. Moreover, because performance antibodies varies among assay types conditions, detailed information on variables protocols is also value. To...
Abstract Little is known about if and how circular RNAs (circRNAs) are involved in skeletal muscle atrophy. Here a conserved RNA Damage‐specific DNA binding protein 1 (circDdb1), derived from the host gene encoding (DDB1), as mechanism of atrophy identified. circDdb1 expression markedly increased variety types vivo vitro, human aging muscle. Both ectopic causes In contrast, multiple forms caused by dexamethasone, tumor necrosis factor‐alpha (TNF‐α), or angiotensin II (Ang II) myotube cells,...
Aims: Metabolic remodeling of cardiac muscles during pathological hypertrophy is characterized by downregulation fatty acid oxidation (FAO) regulator, peroxisome proliferator-activated receptor alpha (PPARα). Thereby, we hypothesized that a cardiac-specific induction PPARα might restore the FAO-related protein expression and resultant energy deficit. In present study, consequences augmentation were evaluated for amelioration chronic oxidative stress, myocyte apoptosis, function hypertrophy....
ABSTRACT Exosomes are small extracellular vesicles (sEVs) of ∼30-150 nm in diameter that have the same topology as cell, enriched selected exosome cargo proteins, and play important roles health disease. To address large unanswered questions regarding biology vivo , we created exomap1 transgenic mouse model. In response to Cre recombinase, mice express HsCD81mNG, a fusion protein between human CD81, most highly yet described, bright green fluorescent mNeonGreen. As expected, cell...
Mitochondria play a significant role in the functioning of cell, acting both as an intracellular energy source and signaling organelle. In heart, mitochondria produce more than 95% ATP addition to regulating redox state, calcium homeostasis, apoptosis, lipid synthesis. However, there is also evidence that exist outside cells where they have been observed mediate cell-to-cell communication, repair, participate immune response. Cardiometabolic disease (CMD) has complex pathogenesis closely...
ABSTRACT We characterized circulating extracellular vesicles (EVs) in obese and lean humans, identifying transcriptional cargo differentially expressed obesity. Since EVs may have broad origin, we compared this obesity EV transcriptome to expression from human visceral adipose tissue derived freshly collected cultured biopsies the same individuals. Using a comprehensive set of adipose-specific epigenomic chromatin conformation assays, found that transcripts were those regulated by...
Atherosclerosis, formed by fibrofatty lesions in the artery wall, underpins myocardial infarctions, strokes and disabling peripheral diseases. Inflammation with multiple maladaptive roles runs through atherosclerotic inception, propagation, complications. Given degree of inflammation differing among atherosclerotics, inflammation-targeted nanomedicine may produce individualized monitoring manipulation inflammatory regulators beyond traditionally slowing down atherosclerosis progression....
SUMMARY Metabolic dysfunction-associated steatotic liver disease (MASLD) prevalence is increasing in parallel with an obesity pandemic, calling for novel strategies prevention and treatment. We defined a circulating proteome of human MASLD across ≈7000 proteins ≈5000 individuals from diverse, at-risk populations the metabolic health spectrum, demonstrating reproducible diagnostic performance specifying both known pathways relevant to (central carbon amino acid metabolism, hepatocyte...
SUMMARY The ability to track disease without tissue biopsy in patients is a major goal biology and medicine. Here, we identify characterize cardiomyocyte-derived extracellular vesicles circulation (EVs; “cardiovesicles”) through comprehensive studies of induced pluripotent stem cell-derived cardiomyocytes, genetic mouse models, state-of-the-art mass spectrometry low-input transcriptomics. These identified two markers ( POPDC2 , CHRNE ) enriched on cardiovesicles for biotinylated...
ABSTRACT Background Acute decompensation is associated with increased mortality in heart failure (HF) patients, though the underlying etiology remains unclear. Extracellular vesicles (EVs) and their cargo may mark specific cardiovascular physiologic states. We hypothesized that EV transcriptomic cargo, including long non-coding RNAs (lncRNAs) mRNAs, dynamic from decompensated to recompensated HF state, reflecting molecular pathways relevant adverse remodeling. Methods examined differential...
Sarcoidosis is an inflammatory granulomatous disease of unknown etiology involving any organ or tissue along with combination active sites, even the most silent ones clinically. The unpredictable nature sites involved in sarcoidosis dictates highly variable natural history and necessity to cluster cases at diagnosis based on clinical and/or imaging common characteristics attempt classify patients their more homogeneous phenotypes, possibly similar behavior, prognosis, outcome, therefore...
Abstract Background Cardiorenal syndrome (CRS)—renal injury during heart failure (HF)—is linked to higher morbidity. Whether circulating extracellular vesicles (EVs) and their RNA cargo directly impact its pathogenesis remains unclear. Methods Using a microfluidic kidney chip model (KC), we investigated transcriptional effects of EVs from patients with CRS on renal epithelial/endothelial cells. We used small RNA-seq regression prioritize subsets EV miRNAs associated serum creatinine,...