A5023188085- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Exercise and Physiological Responses
- Lymphatic System and Diseases
- Lipid metabolism and biosynthesis
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Skin and Cellular Biology Research
- FOXO transcription factor regulation
- Muscle metabolism and nutrition
- Diagnosis and Treatment of Venous Diseases
- Cancer, Hypoxia, and Metabolism
- Retinoids in leukemia and cellular processes
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- Fatty Acid Research and Health
- Estrogen and related hormone effects
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Body Contouring and Surgery
- Lymphatic Disorders and Treatments
- Regulation of Appetite and Obesity
Beth Israel Deaconess Medical Center
2021-2025
Harvard University
2021-2025
Broad Institute
2021-2025
Hadassah Medical Center
2025
University of Chicago
2010-2024
University of Michigan
2015-2020
Washtenaw Community College
2017
Inflammation has profound but poorly understood effects on metabolism, especially in the context of obesity and nonalcoholic fatty liver disease (NAFLD). Here, we report that hepatic interferon regulatory factor 3 (IRF3) is a direct transcriptional regulator glucose homeostasis through induction Ppp2r1b , component serine/threonine phosphatase PP2A, subsequent suppression production. Global ablation IRF3 mice high-fat diet protected against both steatosis dysglycemia, whereas...
Adipocyte size and fragility commercial kit costs impose significant limitations on single-cell RNA sequencing of adipose tissue. Accordingly, we developed a workflow to isolate sample-barcode nuclei from individual tissue samples, integrating flow cytometry for quality control, counting, precise pooling direct loading onto the popular 10× Chromium controller. This approach can eliminate batch confounding, significantly reduces poor-quality nuclei, ambient contamination, droplet...
Chronic lymphedema is a progressive, disfiguring disease that results from dysfunction of the lymphatic vasculature, causing distal accumulation interstitial fluid, localized development tissue edema, and expansion subcutaneous adipose (SAT). As molecular mechanisms governing SAT remodeling in this are unclear, we performed single-nucleus RNA sequencing on paired control affected biopsies patients with unilateral lymphedema. Lymphedema samples were characterized by SAA + adipocytes,...
Adipose tissue is a central player in energy balance and glucose homeostasis, expanding the face of caloric overload order to store safely. If continues unabated, however, adipose becomes dysfunctional, leading systemic metabolic compromise form insulin resistance type 2 diabetes. Changes during development disease are varied complex, made all more so by heterogeneity cell types within tissue. Here we present detailed comparisons atlases murine WAT setting diet-induced obesity, as well after...
Obesity is a global epidemic that contributes to the increasing medical burdens related type 2 diabetes, cardiovascular disease and cancer. A better understanding of mechanisms regulating adipose tissue expansion could lead therapeutics eliminate or reduce obesity-associated morbidity mortality. The extracellular matrix (ECM) has been shown regulate development function numerous tissues organs. However, there little its in tissue. In this manuscript we describe role laminin α4, specialized...
It has long been recognized that body fat distribution and regional adiposity play a major role in the control of metabolic homeostasis. However, ability to study compare cell autonomous regulation response adipocytes from different depots hampered by difficulty inducing preadipocytes isolated visceral depot differentiate into mature culture. Here, we present an easily created 3-dimensional (3D) culture system can be used as robustly those sc depot. The cells differentiated these 3D collagen...
Visceral adipose tissue (VAT) depots are associated with the adverse metabolic consequences of obesity, such as insulin resistance. The developmental origin VAT and identity regulation adipocyte progenitor cells have been active areas investigation. In recent years, a paradigm mesothelial source has emerged based on lineage tracing studies using Wilms' tumor gene, Wt1, marker for origin. Here, we show that Wt1 expression in is not limited to mesothelium but also expressed by distinct...
Abstract It has been reported that class I histone deacetylase (HDAC) inhibition increases thermogenesis in fat, but adipocyte-specific Hdac3 deletions have presented inconsistent results. In this study, we observed HDAC3 protein levels were lower brown fat compared with inguinal subcutaneous adipose tissue, and they decreased both depots upon cold exposure. PR domain–containing 16 (PRDM16) physically interacted HDAC3, treatment HDAC3-selective inhibitor RGFP966 induced thermogenic gene...
ABSTRACT We characterized circulating extracellular vesicles (EVs) in obese and lean humans, identifying transcriptional cargo differentially expressed obesity. Since EVs may have broad origin, we compared this obesity EV transcriptome to expression from human visceral adipose tissue derived freshly collected cultured biopsies the same individuals. Using a comprehensive set of adipose-specific epigenomic chromatin conformation assays, found that transcripts were those regulated by...
Brief summary: Emont et al. created a cell atlas of human and mouse white adipose tissue, which goes long way in informing our understanding the complexity tissue.