A5079170618- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Ethics in Clinical Research
- Epigenetics and DNA Methylation
- Diabetes Treatment and Management
- Nutrition and Health in Aging
- Bariatric Surgery and Outcomes
- Bipolar Disorder and Treatment
- Diabetes Management and Research
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
- BRCA gene mutations in cancer
- Genomics and Rare Diseases
- Muscle Physiology and Disorders
- Enhanced Recovery After Surgery
- Regulation of Appetite and Obesity
- Single-cell and spatial transcriptomics
- Asthma and respiratory diseases
- Nutrition, Genetics, and Disease
- Fatty Acid Research and Health
- IL-33, ST2, and ILC Pathways
Mayo Clinic in Arizona
2015-2025
Mayo Clinic Hospital
2021-2023
Beth Israel Deaconess Medical Center
2018-2021
Harvard University
2018
Mayo Clinic in Florida
2015
Arizona State University
2006-2011
The University of Texas Health Science Center at San Antonio
2006-2011
Texas Diabetes Institute
2006
Endocrinology Research Center
2005
Mitochondrial dysfunction, associated with insulin resistance, is characterized by low expression of peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) and nuclear-encoded mitochondrial genes. This deficit could be due to decreased physical activity or a response gene exercise. The objective this study was investigate whether bout exercise induces the same increase in insulin-sensitive insulin-resistant subjects matched for capacity. Seven lean nine obese took...
Abstract Objective: The present study was undertaken to determine whether improved vasodilatory function accompanies increased insulin sensitivity in overweight, insulin-resistant subjects (OW) and type 2 diabetic patients (T2DM) who participated an 8-wk exercise training regimen. Design: Before after training, had euglycemic clamps sensitivity. Brachial artery catheterization done on another occasion for measurement of function. A lean, healthy, untrained group studied as nonexercised...
Peripheral edema, mild weight gain, and anemia are often observed in type II diabetic patients treated with thiazolidinediones (TZDs). Small decreases hemoglobin (Hb) hematocrit (Hct) appear to be a class effect of TZDs generally attributed fluid retention, although experimental data lacking. We analyzed 50 diabetes mellitus undergoing either placebo or pioglitazone (PIO, 45 mg/day) for 16 weeks. Before after therapy, we measured Hb/Hct used 3H2O bioimpedance quantitate total body water...
BACKGROUND. Obesity is a multifactorial disease with adverse health implications including insulin resistance (IR). In patients obesity, the presence of high circulating levels leptin, deemed hyperleptinemia, associated IR. Recent data in mice diet-induced obesity (DIO) show that partial reduction leptin improves Additional animal studies demonstrate IL-4 decreases levels. rodents, resident adipose tissue eosinophils (AT-EOS) are main source and instrumental maintaining metabolic...
Purpose The Sangre Por Salud (SPS) Biobank was established to facilitate biomedical research opportunities for the Latino community by creating an easily accessible prospective cohort scientists interested in studying health conditions and disparities this population. Participants Individuals self-identifying as Latino, aged 18–85 years, were prospectively recruited from primary care Internal Medicine clinic at Mountain Park Health Center Phoenix, Arizona. After obtaining informed consent,...
The growing prevalence of obesity, a risk factor for disorders such as Alzheimer's Disease (AD), raises concerns about the effects on cognitive health. AD currently impacts 6.9 million Americans aged 65 and older is characterized by presence amyloid beta (Aβ) plaques, neurofibrillary tau tangles, neuroinflammation, all which contribute to impairment. Insulin resistance, common in both obesity AD, disrupts brain glucose metabolism accelerates neurodegeneration. Understanding factors that link...
Bisphenol-A (BPA) is a lipophilic compound widely used in the manufacture of plastic items and thought to play role growing obesity epidemic. Recent publications suggest that BPA may have pro-adipogenic effect. Here we explore effect low, but environmentally relevant, concentrations on adipogenesis using variety cellular models. Mouse 3T3-L1, C3H10T1/2 human adipose-derived stromal cells (hADSCs) were cultured with ranging from 0.1nM 100μM. We failed observe positive effects differentiation...
Skeletal muscle is highly plastic and dynamically regulated by the body's physical demands. This study aimed to determine plasticity of skeletal DNA methylation in response 8 weeks supervised exercise training volunteers with a range insulin sensitivities. We studied 13 sedentary participants performed euglycemic hyperinsulinemic clamps basal vastus lateralis biopsies peak aerobic activity (VO2 peak) tests before after training. extracted from global using Illumina's Methylation EPIC 850K...
The adiponectin signalling pathway is largely unknown, but recently the adaptor protein containing pleckstrin homology domain, phosphotyrosine binding domain and leucine zipper motif (APPL1), has been shown to interact directly with receptor (ADIPOR)1. APPL1 present in C2C12 myoblasts mouse skeletal muscle, its presence human muscle not investigated. Samples from type 2 diabetic, lean non-diabetic obese participants were analysed by: immunoprecipitation western blot; HPLC-electrospray...
Abstract Background Long chain omega-3 polyunsaturated fatty acids (ω-3PUFA) supplementation in animal models of diet-induced obesity has consistently shown to improve insulin sensitivity. The same is not always reported human studies with resistant (IR) subjects obesity. Objective We studied whether high-dose ω-3PUFA for 3 months improves sensitivity and adipose tissue (AT) inflammation IR Methods Thirteen (BMI = 39.3 ± 1.6 kg/m 2 ) underwent 80 mU/m ·min euglycemic-hyperinsulinemic clamp...
Abstract Context Metformin is the first-line drug for treating diabetes but has a high failure rate. Objective To identify demographic and clinical factors available in electronic health record (EHR) that predict metformin failure. Methods A cohort of patients with at least 1 abnormal screening test initiated was identified 3 sites (Arizona, Mississippi, Minnesota). We 22 047 initiators (48% female, mean age 57 ± 14 years) including 2141 African Americans, 440 Asians, 962 Other/Multiracial,...
<b><i>Aim:</i></b> To develop a process for returning medically actionable genomic variants to Latino patients receiving care in Federally Qualified Health Center. <b><i>Methods:</i></b> Prior recruitment, researchers met with primary providers (1) orient clinicians the project, (2) establish and nonactionable results participants through electronic health record, (3) offering clinical decision support follow-up education care. A Community...
<b><i>Background/Aims:</i></b> The Sangre Por Salud (Blood for Health; SPS) Biobank was created the purpose of expanding precision medicine research to include underrepresented Latino patients. It is result a unique collaboration between Mayo Clinic and Mountain Park Health Center, federally qualified community health center in Phoenix, Arizona. This report describes rationale, development, implementation, characteristics SPS Biobank....
Our previous studies show reduced abundance of the β-subunit mitochondrial H+-ATP synthase (β-F1-ATPase) in skeletal muscle obese individuals. The β-F1-ATPase forms catalytic core ATP synthase, and it is critical for production muscle. mechanism(s) impairing metabolism obesity, however, are not completely understood. First, we studied total protein synthesis translation efficiency (BMI, 36±1 kg/m2) lean 22±1 subjects. Both (0.044±0.006 vs 0.066±0.006%·h-1) (0.0031±0.0007 0.0073±0.0004) were...
OBJECTIVE—We sought to examine the mechanisms by which addition of glargine insulin or rosiglitazone improves glycemic control in type 2 diabetic subjects poorly controlled on maximally effective doses metformin plus sulfonylurea. RESEARCH DESIGN AND METHODS—Subjects (aged 47 ± 11 years, BMI 31 5 kg/m2, HbA1c [A1C] 9.4 1.3%) received bedtime (titrated based fasting plasma glucose [FPG], n = 10) (4 mg twice daily, 10). At baseline and after 4 months, A1C was measured an oral tolerance test a...
Abstract Context Humans with obesity and insulin resistance exhibit lipid accumulation in skeletal muscle, but the underlying biological mechanisms responsible for of muscle these individuals remain unknown. Objective We investigated how plasma modulates extraction circulating triglycerides (TGs) nonesterified fatty acids (NEFAs) from ingested endogenous origin lean, insulin-sensitive humans (Lean-IS) contrasted responses to those (Obese-IR). Methods The studies were performed a postprandial...
The mechanisms of weight loss and metabolic improvements following bariatric surgery in skeletal muscle are not well known; however, epigenetic modifications likely to contribute. aim our study was investigate DNA methylation after induced by Roux-en-Y gastric bypass (RYGB) surgery. Muscle biopsies were obtained basally from seven insulin-resistant obese (BMI > 40 kg/m2) female subjects (45.1 ± 3.6 years) pre- 3-month post-surgery with euglycemic hyperinsulinemic clamps assess insulin...
Abstract Context Humans with obesity and insulin resistance exhibit lipid accumulation in skeletal muscle, but the underlying biological mechanisms responsible for of muscle these individuals remain unknown. Objective We investigated how plasma modulates extraction circulating triglycerides (TGs) non-esterified fatty acids (NEFAs) from ingested endogenous origin lean, insulin-sensitive humans (Lean-IS) contrasted responses to those (Obese-IR). Methods The studies were performed a...