A5025932423- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Single-cell and spatial transcriptomics
- Metabolism, Diabetes, and Cancer
- Congenital gastrointestinal and neural anomalies
- Intestinal Malrotation and Obstruction Disorders
- Atherosclerosis and Cardiovascular Diseases
- Lymphatic System and Diseases
- Neurogenesis and neuroplasticity mechanisms
- Liver Disease Diagnosis and Treatment
- Lymphatic Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Gastrointestinal motility and disorders
- Regulation of Appetite and Obesity
- TGF-β signaling in diseases
- Cardiovascular Disease and Adiposity
- Diagnosis and Treatment of Venous Diseases
- Neurobiology and Insect Physiology Research
- Diet, Metabolism, and Disease
Hadassah Medical Center
2022-2025
Beth Israel Deaconess Medical Center
2021-2025
Harvard University
2022-2024
Adipose tissue is a central player in energy balance and glucose homeostasis, expanding the face of caloric overload order to store safely. If continues unabated, however, adipose becomes dysfunctional, leading systemic metabolic compromise form insulin resistance type 2 diabetes. Changes during development disease are varied complex, made all more so by heterogeneity cell types within tissue. Here we present detailed comparisons atlases murine WAT setting diet-induced obesity, as well after...
Inflammation has profound but poorly understood effects on metabolism, especially in the context of obesity and nonalcoholic fatty liver disease (NAFLD). Here, we report that hepatic interferon regulatory factor 3 (IRF3) is a direct transcriptional regulator glucose homeostasis through induction Ppp2r1b , component serine/threonine phosphatase PP2A, subsequent suppression production. Global ablation IRF3 mice high-fat diet protected against both steatosis dysglycemia, whereas...
Adipocyte size and fragility commercial kit costs impose significant limitations on single-cell RNA sequencing of adipose tissue. Accordingly, we developed a workflow to isolate sample-barcode nuclei from individual tissue samples, integrating flow cytometry for quality control, counting, precise pooling direct loading onto the popular 10× Chromium controller. This approach can eliminate batch confounding, significantly reduces poor-quality nuclei, ambient contamination, droplet...
Maintenance of normal structure the enteric nervous system (ENS), which regulates key gastrointestinal functions, requires robust homeostatic mechanisms, since by virtue its location within gut wall, ENS is subject to constant mechanical, chemical, and biological stressors. Using transgenic thymidine analogue-based experiments, we previously discovered that neuronal turnover – where continual neurogenesis offsets ongoing loss at steady state represents one such mechanism. Although other...
Chronic lymphedema is a progressive, disfiguring disease that results from dysfunction of the lymphatic vasculature, causing distal accumulation interstitial fluid, localized development tissue edema, and expansion subcutaneous adipose (SAT). As molecular mechanisms governing SAT remodeling in this are unclear, we performed single-nucleus RNA sequencing on paired control affected biopsies patients with unilateral lymphedema. Lymphedema samples were characterized by SAA + adipocytes,...
Brief summary: Emont et al. created a cell atlas of human and mouse white adipose tissue, which goes long way in informing our understanding the complexity tissue.
Abstract Maintenance of normal structure the enteric nervous system (ENS), which regulates key gastrointestinal functions, requires robust homeostatic mechanisms, since by virtue its location within gut wall, ENS is subject to constant mechanical, chemical, and biological stressors. Using transgenic thymidine analogue-based experiments, we previously discovered that neuronal turnover – where continual neurogenesis offsets ongoing loss at steady state represents one such mechanism. Although...
ABSTRACT White adipose tissue (WAT), once regarded as morphologically and functionally bland, is now recognized to be dynamic, plastic, heterogenous, involved in a wide array of biological processes including energy homeostasis, glucose lipid handling, blood pressure control, host defense 1 . High fat feeding other metabolic stressors cause dramatic changes morphology, physiology, cellular composition , alterations adiposity are associated with insulin resistance, dyslipidemia, type 2...
ABSTRACT AgRP neurons in the arcuate nucleus of hypothalamus (ARC) coordinate homeostatic changes appetite associated with fluctuations food availability and leptin signaling. Identifying relevant transcriptional regulatory pathways these has been a priority, yet such attempts have stymied due to their low abundance rich cellular diversity ARC. Here we generated neuron-specific transcriptomic chromatin accessibility profiles during opposing states fasting-induced hunger leptin-induced...
ABSTRACT Growth differentiation factor 3 (GDF3) is a relatively understudied member of the TGFβ superfamily that highly expressed during development. However, function GDF3 in adult biology contentious. We use vivo approaches to show loss-of-function adipose tissue obese mice causes reduced body weight and improved whole-body insulin sensitivity. These effects are accompanied by altered regulation genes targeted . Using vitro approaches, we can influence both arms superfamily: simultaneously...