A5035552644- Extracellular vesicles in disease
- RNA Research and Splicing
- RNA modifications and cancer
- RNA regulation and disease
- Amyotrophic Lateral Sclerosis Research
- Microbial Community Ecology and Physiology
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Methane Hydrates and Related Phenomena
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Caveolin-1 and cellular processes
- Alzheimer's disease research and treatments
- Hydrocarbon exploration and reservoir analysis
- Liver Disease Diagnosis and Treatment
- Neurogenetic and Muscular Disorders Research
- CRISPR and Genetic Engineering
- Atmospheric and Environmental Gas Dynamics
- Atherosclerosis and Cardiovascular Diseases
- Genetics and Physical Performance
- Metabolomics and Mass Spectrometry Studies
- Genetic Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
Translational Genomics Research Institute
2018-2025
City Of Hope National Medical Center
2023
City of Hope
2023
Shell (United States)
2016-2019
Joint Genome Institute
2016-2019
Shell (France)
2017
Arizona State University
2012-2015
Developing strategies that promote the resolution of vascular inflammation and atherosclerosis remains a major therapeutic challenge. Here, we show exosomes produced by naive bone marrow-derived macrophages (BMDM-exo) contain anti-inflammatory microRNA-99a/146b/378a are further increased in BMDM polarized with IL-4 (BMDM-IL-4-exo). These exosomal microRNAs suppress targeting NF-κB TNF-α signaling foster M2 polarization recipient macrophages. Repeated infusions BMDM-IL-4-exo into Apoe−/− mice...
Subsurface petroleum reservoirs are an important component of the deep biosphere where indigenous microorganisms live under extreme conditions and in isolation from Earth's surface for millions years. However, unlike bulk biosphere, reservoir is subject to anthropogenic perturbation, with introduction new electron acceptors, donors exogenous microbes during oil exploration production. Despite fundamental practical significance this there has never been a systematic evaluation ecological...
ABSTRACT Offshore oil production facilities are frequently victims of internal piping corrosion, potentially leading to human and environmental risks significant economic losses. Microbially influenced corrosion (MIC) is believed be an important factor in this major problem for the petroleum industry. However, knowledge microbial communities metabolic processes still limited. Therefore, from three anaerobic biofilms recovered inside a steel pipe exhibiting high rates, iron oxide deposits,...
Previous translational studies implicate plasma extracellular microRNA-30d (miR-30d) as a biomarker in left ventricular remodeling and clinical outcome heart failure (HF) patients, although precise mechanisms remain obscure.
The Foundational Data Initiative for Parkinson Disease (FOUNDIN-PD) is an international collaboration producing fundamental resources disease (PD). FOUNDIN-PD generated a multi-layered molecular dataset in cohort of induced pluripotent stem cell (iPSC) lines differentiated to dopaminergic (DA) neurons, major affected type PD. were derived from the Parkinson's Progression Markers study, which included participants with PD carrying monogenic variants, variants intermediate effects, and...
Abstract The C9ORF72 -linked diseases amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are characterized by the nuclear depletion cytoplasmic accumulation of TAR DNA-binding protein 43 (TDP-43). Recent studies have shown that loss TDP-43 function leads to inclusion cryptic exons (CE) in several RNA transcript targets TDP-43. Here, we show for first time detection CEs a single-nuclei sequencing (snRNA-seq) dataset obtained from frontal occipital cortices patients...
The emergence of single nucleus RNA sequencing (snRNA-seq) offers to revolutionize the study Alzheimer's disease (AD). Integration with complementary multiomics data such as genetics, proteomics and clinical provides powerful opportunities link cell subpopulations molecular networks a broader disease-relevant context. We report snRNA-seq profiles from superior frontal gyrus samples 101 well characterized subjects Banner Brain Body Donation Program in combination whole genome sequences....
We have constructed a conceptual model of biogeochemical cycles and metabolic microbial community shifts within hot spring ecosystem via coordinated analysis the "Bison Pool" (BP) Environmental Genome complementary contextual geochemical dataset ∼75 parameters. 2,321 16S rRNA clones 470 megabases environmental sequence data were produced from biofilms at five sites along outflow BP, an alkaline in Sentinel Meadow (Lower Geyser Basin) Yellowstone National Park. This channel acts as >22 m...
Evolving interest in comprehensively profiling the full range of small RNAs present tissue biopsies and circulating biofluids, how profile differs with disease, has launched RNA sequencing (RNASeq) into more frequent use. However, known biases associated RNASeq, compounded by low inputs, have been both a significant concern hurdle to widespread adoption. As RNASeq is becoming viable choice for discovery input samples labs are employing it, there should be benchmark datasets test evaluate...
Abstract Oil and gas percolate profusely through the sediments of Gulf Mexico, leading to numerous seeps at seafloor, where complex microbial, sometimes animal communities flourish. Sediments from three areas (two cold with contrasting hydrocarbon composition a site outside any area active seepage) Mexico were investigated compared. Consistent existence seep microbiome, distinct microbial community was observed in compared sediment seepage. The without influence seepage characterized by...
Rapid identification of patients suffering from cerebral ischaemia, while excluding intracerebral haemorrhage, can assist with patient triage and expand access to chemical mechanical revascularization. We sought identify blood-based, extracellular microRNAs 15 (ex-miRNAs) derived vesicles associated major stroke subtypes using clinical samples subjects spontaneous intraparenchymal haemorrhage (IPH), aneurysmal subarachnoid (SAH) ischaemic due vessel occlusion. collected blood presenting IPH...
Abstract Biomarkers that are clinically useful for the diagnosis and treatment of schizophrenia lacking. critical tools reduce incidence misdiagnosis, identify subgroups patients, assist in proper characterization patient phenotypes, predict response to or development side effects, can serve as targets novel therapeutic interventions. In this study, we evaluated small (< 200 nucleotide) long (> RNAs found extracellular vesicles (EVs) isolated from cerebrospinal fluid (CSF) plasma...
We investigated whether extracellular vesicles (EVs) produced under hyperglycemic conditions could communicate signaling to drive atherosclerosis. did so by treating Apoe-/- mice with exosomes bone marrow-derived macrophages (BMDM) exposed high glucose (BMDM-HG-exo) or control. Infusions of BMDM-HG-exo increased hematopoiesis, circulating myeloid cell numbers, and atherosclerotic lesions an accumulation macrophage foam apoptotic cells. Transcriptome-wide analysis cultured treated plasma EVs...
While motor and cortical neurons are affected in C9orf72 amyotrophic lateral sclerosis frontotemporal dementia (ALS/FTD), it remains largely unknown if how non-neuronal cells induce or exacerbate neuronal damage. We differentiated ALS/FTD patient-derived induced pluripotent stem into microglia (iPSC-MG) examined their intrinsic phenotypes. Similar to iPSC neurons, iPSC-MG mono-cultures form G 4 C 2 repeat RNA foci, exhibit reduced protein levels, generate dipeptide proteins. Healthy control...
We examined small and long transcriptomics in skeletal muscle serum-derived extracellular vesicles before after a single exposure to traditional combined exercise (TRAD) high-intensity tactical training (HITT). Across 40 young adults, we found more consistent protein-coding gene responses TRAD, whereas HITT elicited differential expression of microRNA enriched brain regions. Follow-up analysis revealed relationships temporal dynamics across transcript networks, highlighting potential avenues...
The majority of exercise physiology research has been conducted in males, resulting a skewed biological representation how impacts the physiological system. Extrapolating male-centric findings to females is not universally appropriate and may even be detrimental. Thus, addressing this imbalance taking into consideration sex as variable mandatory for optimization precision interventions and/or regimens. Our present analysis focused on establishing multiomic profiles young, exercise-naïve...
Gulf of Mexico sediments harbor numerous hydrocarbon seeps associated with high sedimentation rates and thermal maturation organic matter. These ecosystems host abundant diverse microbial communities that directly or indirectly metabolize components the emitted fluid. To investigate function activities in these ecosystems, metabolic potential (metagenomic) gene expression (metatranscriptomic) analyses two cold seep areas were carried out. Seeps emitting biogenic methane harbored dominated by...
The Pennsylvania region hosts numerous oil and gas reservoirs the presence of hydrocarbons in groundwater has been locally observed. However, these methane-containing freshwater ecosystems remain poorly explored despite their potential importance carbon cycle. Methane isotope analysis low molecular weight hydrocarbon gases from 18 water wells indicated that active methane cycling may be occurring region. Consistent with this observation, multigenic qPCR gene sequencing (16S rRNA genes, mcrA...