A5081797812- Pluripotent Stem Cells Research
- Amyotrophic Lateral Sclerosis Research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Alzheimer's disease research and treatments
- Peptidase Inhibition and Analysis
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
German Center for Neurodegenerative Diseases
2020-2023
University of Aveiro
2015
Instituto Gulbenkian de Ciência
2015
The Foundational Data Initiative for Parkinson Disease (FOUNDIN-PD) is an international collaboration producing fundamental resources disease (PD). FOUNDIN-PD generated a multi-layered molecular dataset in cohort of induced pluripotent stem cell (iPSC) lines differentiated to dopaminergic (DA) neurons, major affected type PD. were derived from the Parkinson's Progression Markers study, which included participants with PD carrying monogenic variants, variants intermediate effects, and...
Summary The Sn RK 1 protein kinase balances cellular energy levels in accordance with extracellular conditions and is thereby key for plant stress tolerance. In addition, has been implicated numerous growth developmental processes from seed filling maturation to flowering senescence. Despite its importance, the mechanisms that regulate activity are poorly understood. Here, we demonstrate complex SUMO ylated on multiple subunits identify SIZ as E3 Small Ubiquitin‐like Modifier ( ) ligase...
Small non-coding RNAs (sncRNAs) are a class of transcripts implicated in several eukaryotic regulatory mechanisms, namely gene silencing and chromatin regulation. Despite significant progress their identification by next generation sequencing (NGS) we still far from understanding full diversity functional repertoire. Here report the tRNA derived fragments (tRFs) NGS sncRNA fraction zebrafish. The tRFs identified 18–30 nt long, specific 5′ 3′ processing mature tRNAs differentially expressed...
Manual culture and differentiation protocols for human induced pluripotent stem cells (hiPSC) are difficult to standardize, show high variability prone spontaneous into unwanted cell types. The methods labor-intensive not easily amenable large-scale experiments. To overcome these limitations, we developed an automated system coupled a high-throughput imaging implemented maintaining multiple hiPSC lines in parallel neuronal differentiation. We describe the automation of short-term protocol...
Abstract Understanding the molecular mechanisms underlying frontotemporal dementia (FTD) is essential for development of successful therapies. Systematic studies on human post-mortem brain tissue patients with genetic subtypes FTD are currently lacking. The Risk and Modyfing Factors Frontotemporal Dementia (RiMod-FTD) consortium therefore has generated a multi-omics dataset to identify common distinct disturbed in disease. Here, we present datasets from frontal lobe mutations MAPT, GRN...
Abstract Despite extensive research, the contribution of LRRK2 p.G2019S mutation to Parkinson’s disease (PD) remains unclear. Recent findings indicate oligodendrocytes (ODCs) and their progenitors are vulnerable in PD pathogenesis. Notably, oligodendrocyte precursor cells (OPCs) exhibit high endogenous expression . We induced patient-iPSCs with into oligodendroglial lineages performed single-cell RNA sequencing. Cell type composition analysis revealed an increase OPCs, proliferating OPCs...
Abstract Understanding the molecular mechanisms underlying frontotemporal dementia (FTD) is essential for development of successful therapies. Here we integrated transcriptomic and epigenomic analyses postmortem human brains FTD patients with mutations in MAPT, GRN C9orf72 detected common distinct dysregulated cellular pathways between patient groups. Our results highlight that excitatory neurons are most vulnerable neuronal cell type vascular aberrations a hallmark FTD. Via integration...
Abstract Understanding the molecular mechanisms underlying frontotemporal dementia (FTD) is essential for development of successful therapies. Systematic studies on human post-mortem brain tissue patients with genetic subtypes FTD are currently lacking. The Risk and Modyfing Factors Frontotemporal Dementia (RiMod-FTD) consortium therefore has generated a multi-omics dataset to identify common distinct disturbed in disease. Here, we present datasets from frontal lobe mutations MAPT, GRN...
Manual culture and differentiation protocols for human induced pluripotent stem cells (hiPSC) are difficult to standardize, show high variability prone spontaneous into unwanted cell types. The methods labor-intensive not easily amenable large-scale experiments. To overcome these limitations, we developed an automated system coupled a high-throughput imaging implemented maintaining multiple hiPSC lines in parallel neuronal differentiation. We describe the automation of short-term protocol...
Abstract The FOUNdational Data INitiative for Parkinson’s Disease (FOUNDIN-PD) is an international collaboration producing fundamental resources disease (PD). FOUNDIN-PD generated a multi-layered molecular dataset in cohort of induced pluripotent stem cell (iPSC) lines differentiated to dopaminergic (DA) neurons, major affected type PD. were derived from the Progression Markers Initiative study including participants with PD carrying monogenic ( SNCA ) variants, variants intermediate effects...