A5061133500- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Lipid metabolism and biosynthesis
- Protein Degradation and Inhibitors
- Plant Molecular Biology Research
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Celiac Disease Research and Management
- Food Allergy and Anaphylaxis Research
- Plant responses to water stress
- ATP Synthase and ATPases Research
- Plant Reproductive Biology
- Allergic Rhinitis and Sensitization
- NF-κB Signaling Pathways
- Plant Genetic and Mutation Studies
- DNA Repair Mechanisms
- Polar Research and Ecology
Max Perutz Labs
2012-2022
University of Vienna
2012-2022
Cross-talk between plant cells and their surroundings requires tight regulation of information exchange at the plasma membrane (PM), which involves dynamic adjustments PM protein localization turnover to modulate signal perception solute transport interface surroundings. In animals fungi, proteins is controlled by reversible ubiquitylation, signals endocytosis delivery cell’s lytic compartment, there emerging evidence for related mechanisms in plants. Here, we describe fate Arabidopsis PIN2...
Abstract The Arabidopsis thaliana genes PROTEIN INHIBITOR OF ACTIVATED STAT LIKE1 (PIAL1) and PIAL2 encode proteins with SP-RING domains, which occur in many ligases of the small ubiquitin-related modifier (SUMO) conjugation pathway. We show that PIAL1 function as SUMO capable chain formation require SUMO-modified SUMO-conjugating enzyme SCE1 for optimal activity. Mutant analysis indicates a role 2 salt stress osmotic responses, whereas under standard conditions, mutants close to normal...
Meiosis is a specialized cell division that gives rise to genetically distinct gametic cells. relies on the tightly controlled formation of DNA double-strand breaks (DSBs) and their repair via homologous recombination for correct chromosome segregation. Like all forms damage, meiotic DSBs are potentially harmful activates an elaborate response inhibit excessive break ensure successful repair. Previous studies established protein kinase ATM as DSB sensor regulator in several organisms. Here...
Summary The Sn RK 1 protein kinase balances cellular energy levels in accordance with extracellular conditions and is thereby key for plant stress tolerance. In addition, has been implicated numerous growth developmental processes from seed filling maturation to flowering senescence. Despite its importance, the mechanisms that regulate activity are poorly understood. Here, we demonstrate complex SUMO ylated on multiple subunits identify SIZ as E3 Small Ubiquitin‐like Modifier ( ) ligase...
Summary Conjugation of the small ubiquitin‐related modifier ( SUMO ) to protein substrates has an impact on stress responses and development. We analyzed proteome phosphoproteome mutants in this pathway. The chosen had defects ligase SIZ 1, which catalyzes attachment single moieties onto substrates, ligases PIAL 1 2, are known form chains. A total 2657 proteins 550 phosphopeptides were identified quantified. Approximately 40% 20% showed differences abundance at least one genotypes,...
Post-translational modifications are essential mediators between stimuli from development or the environment and adaptive transcriptional patterns. Recent data allow a first glimpse at how two modifications, phosphorylation sumoylation, act interdependently to modulate stress responses. In particular, many components of SUMO conjugation system phosphoproteins, some regulators enzymes protein can be sumoylated. Equally important, however, number proteins subject both modifications. These...
Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate vivo vitro SUMO conjugating enzyme with Cys Ser change the active site, show that it has dominant negative effect. In planta expression significantly perturbs normal development, leading growth retardation, early flowering gene changes. We suggest mutant protein can serve as probe...
The small ubiquitin-related modifier (SUMO) conjugation apparatus usually attaches single SUMO moieties to its substrates, but chains have also been identified. To better define the biochemical requirements and characteristics of chain formation, mutations in surface-exposed Lys residues Arabidopsis SUMO-conjugating enzyme (SCE) were tested for vitro activity. Lys-to-Arg changes amino-terminal region SCE allowed acceptance from SUMO-activating supported substrate mono-sumoylation, these had...
The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group plant E4 SUMO ligases. This protocol allows quick simple expression recombinant in Escherichia coli (E. coli) subsequent affinity purification using a maltose binding protein (MBP) tag. can be used SUMOylation reactions, where MBP part detected with commercially available antibody, or additional steps applied.