A5008257091- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Plant Pathogens and Fungal Diseases
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Advanced Proteomics Techniques and Applications
- Fungal Infections and Studies
- Banana Cultivation and Research
- Influenza Virus Research Studies
- Peptidase Inhibition and Analysis
- Adipose Tissue and Metabolism
- Circular RNAs in diseases
- Mitochondrial Function and Pathology
- Antifungal resistance and susceptibility
- Cancer-related gene regulation
- Protein Structure and Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Protease and Inhibitor Mechanisms
- Advanced biosensing and bioanalysis techniques
- Autophagy in Disease and Therapy
- Viral Infections and Vectors
- Birth, Development, and Health
University of Aveiro
2015-2024
Administração Regional de Saúde de Lisboa e Vale do Tejo
2024
Hospital de Santa Marta
2024
Universidade Federal do Piauí
2018-2021
Kansas State University
2017-2018
Universidade de São Paulo
2008-2012
University of Coimbra
2009-2010
Universidade Brasil
2010
MicroRNAs (miRNAs) are short (∼22 nt) non-coding regulatory RNAs that control gene expression at the post-transcriptional level. Deregulation of miRNA has been discovered in a wide variety tumours and it is now clear they contribute to cancer development progression. Cervical one most common cancers women worldwide there strong need for non-invasive, fast efficient method diagnose disease. We investigated profiles cervical using microarray platform containing probes mature miRNAs. have...
Transfer RNA (tRNA) molecules contain various post-transcriptional modifications that are crucial for tRNA stability, translation efficiency, and fidelity. Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of non-coding with regulatory functions ranging from regulation to gene expression control cellular stress response. Recent evidence indicates tsRNAs modified, however, the impact epitranscriptome deregulation on generation is only...
Abstract Background MicroRNAs (miRNAs) are a new class of small RNAs approximately 22 nucleotides in length that control eukaryotic gene expression by fine tuning mRNA translation. They regulate wide variety biological processes, namely developmental timing, cell differentiation, proliferation, immune response and infection. For this reason, their identification is essential to understand biology. Their size, low abundance high instability complicated early identification, however...
Autophagy is a key degradative pathway coordinated by external cues, including starvation, oxidative stress, or pathogen detection. Rare are the molecules known to contribute mechanistically regulation of autophagy and expressed specifically in particular environmental contexts distinct cell types. Here, we unravel role RUN FYVE domain-containing protein 4 (RUFY4) as positive molecular regulator macroautophagy primary dendritic cells (DCs). We show that exposure interleukin-4 (IL-4) during...
Prenatal exposure to ethanol leads a myriad of developmental disorders known as fetal alcohol spectrum disorder, often characterized by growth and mental retardation, central nervous system damage, specific craniofacial dysmorphic features. The mechanisms toxicity are not fully understood, but during development affects the expression several genes involved in cell cycle control, apoptosis, transcriptional regulation. MicroRNAs (miRNAs) implicated some these processes, however, it is yet...
Background Codon pair usage (codon context) is a species specific gene primary structure feature whose evolutionary and functional roles are poorly understood. The data available show that codon-context has direct impact on both translation accuracy efficiency, but one does not yet understand how it affects these two variables or whether context biases shape evolution. Methodologies/Principal Findings Here we study using set of 72 orthologous highly conserved genes from bacteria, archaea,...
Small non-coding RNAs (sncRNAs) are a class of transcripts implicated in several eukaryotic regulatory mechanisms, namely gene silencing and chromatin regulation. Despite significant progress their identification by next generation sequencing (NGS) we still far from understanding full diversity functional repertoire. Here report the tRNA derived fragments (tRFs) NGS sncRNA fraction zebrafish. The tRFs identified 18–30 nt long, specific 5′ 3′ processing mature tRNAs differentially expressed...
Protein conformational disorders are characterized by disruption of protein folding and toxic accumulation aggregates. Here we describe a sensitive simple method to follow monitor general aggregation in human cells. Heat shock 27 (HSP27) is an oligomeric small heat that binds keeps unfolded proteins competent state. This high specificity HSP27 for aggregated can be explored living cells fusing it fluorescent as Green Fluorescent (GFP). We have constructed HeLa stable cell line expressing...
Protein synthesis rate and accuracy are tightly controlled by the cell essential for proteome homoeostasis (proteostasis); however, full picture of how mRNA translational factors maintain protein co-translational folding far from being fully understood. To address this question, we evaluated role 70 yeast tRNA-modifying enzyme genes on aggregation used mass spectrometry to identify aggregated proteins. We show that modification uridine at anticodon position 34 (U34) enzymes Elp1, Elp3, Sml3...
Abstract The pathogenic yeast Candida glabrata is intrinsically resilient to azoles and rapidly acquires resistance these antifungals, in vitro vivo. In most cases azole-resistant C. clinical strains encode hyperactive CgPdr1 variants, however, resistant encoding wild-type CgPDR1 alleles have also been isolated, although remaining be disclosed the underlying mechanism. this study, we scrutinized mechanisms of 8 strains, identified along course epidemiological surveys undertaken Portugal....
Reversing protein aggregation within cells may be an important tool to fight protein-misfolding disorders such as Alzheimer's, Parkinson's, and cardiovascular diseases. Here we report the design synthesis of a family steroid–quinoline hybrid compounds based on framework combination approach. This set effectively inhibited Aβ1–42 self-aggregation in vitro by delaying exponential growth phase and/or reducing quantity fibrils steady state. Their disaggregation efficacy was further demonstrated...
Mutations in genes that encode tRNAs, aminoacyl-tRNA syntheases, tRNA modifying enzymes and other interacting partners are associated with neuropathies, cancer, type-II diabetes hearing loss, but how these mutations cause disease is unclear. We have hypothesized levels of decoding error (mistranslation) do not fully impair embryonic development can accelerate cell degeneration through proteome instability saturation the proteostasis network. To test this hypothesis we induced mistranslation...
The epidemiologic triad (epi-triad) classically consists of agent, host, and environment. However, with vector-borne diseases (arthropod-transmitted pathogens), the vector plays a vital role, without there is no sustained pathogen transmission. By not emphasizing importance arthropod vector, role often neglected or underappreciated. complexities are reviewed by describing ecology transmission Japanese encephalitis virus (JEV), causative agent (JE). JE mosquito-borne zoonosis that affects...
Aging | doi:10.18632/aging.203379. Margarida Ferreira, Stephany Francisco, Ana R. Soares, Nobre, Miguel Pinheiro, Andreia Reis, Sonya Neto, João Rodrigues, Nuno Sousa, Gabriela Moura, Manuel A. S. Santos
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by accumulation of β-amyloid aggregates and loss proteostasis. Transfer RNA (tRNA) modifications play crucial role in maintaining proteostasis, but their impact AD remains unclear. Here, we report that expression the tRNA modifying enzyme ELP3 reduced brain patients amyloid mouse models negatively correlates with plaque mean density. We further show SH-SY5Y neuronal cells carrying amyloidogenic Swedish familial mutation...