A5022701659- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Molecular Biology Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Cystic Fibrosis Research Advances
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Child Nutrition and Feeding Issues
- Bacterial Genetics and Biotechnology
- Respiratory and Cough-Related Research
- Microbial Community Ecology and Physiology
- Infections and bacterial resistance
- HIV/AIDS oral health manifestations
- Ecology and biodiversity studies
- Sensory Analysis and Statistical Methods
- Plant biochemistry and biosynthesis
- Phytase and its Applications
- Enterobacteriaceae and Cronobacter Research
- Amoebic Infections and Treatments
- Oral and gingival health research
- SARS-CoV-2 detection and testing
- Glycosylation and Glycoproteins Research
- Supply Chain Resilience and Risk Management
Universidade Católica Portuguesa
2018-2024
HealthInsight
2022
Instituto Superior Miguel Torga
2021
University of Coimbra
2009-2020
Universidade Federal de Pernambuco
2016
Universidade Lusófona
2013
Abstract The widespread occurrence of clinically relevant antibiotic resistance within humans, animals, and environment motivates the development sensitive accurate detection quantification methods. Metagenomics quantitative PCR (qPCR) are amongst most used approaches. In this study, we aimed to evaluate compare performance these methods screen genes in animal faecal, wastewater, water samples. Water wastewater samples were from hospital effluent, different treatment stages two plants,...
The economic and social environment caused by the COVID-19 pandemic has brought additional pressure on supply chains because they now have more to develop adapt a context of constraint. Delays in chain can bring consequences such as lack food products retailers, transporters manufacturers. Thus, this study aims examine during small- medium-sized companies central region Portugal, identifying potential problems pointing out respective solutions. For purpose, uses qualitative methodology...
Klebsiella pneumoniae are ubiquitous bacteria and recognized multidrug-resistant opportunistic pathogens that can be released into the environment, mainly through sewage, where they survive even after wastewater treatment. A major question is if once wastewater, selection of lineages missing clinically-relevant traits may occur. Wastewater (n = 25) clinical 34) 3rd generation cephalosporin-resistant K. isolates were compared based on phenotypic, genotypic genomic analyses.Clinical...
Cynara cardunculus, Fraxinus angustifolia and Pterospartum tridentatum are plants commercialized in Portugal for digestion-related ailments, among others. The aim of this study was to identify the chemical composition infusions determine inhibition acetylcholinesterase, antioxidant activity, evaluate if activities remained after vitro gastrointestinal digestion. These can be related with traditional uses these plants. toxicity also tested Caco-2 cells, a model intestinal cells. plant...
In this study we aimed to characterize antimicrobial resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bloodstream infections as well the associated genetic lineages of isolates. Sixteen MRSA isolates were recovered bacteremia samples inpatients between 2016 and 2019. The susceptibility these was tested by Kirby–Bauer disk diffusion method against 14 agents. To determine macrolide–lincosamide–streptogramin B (MLSB) phenotype isolates, erythromycin-resistant...
An extracellular collagenolytic serine protease was purified from Aspergillus sp., isolated the Caatinga biome in northeast Brazil by a two-step chromatographic procedure, using an anion-exchanger and gel filtration. The enzyme produced submerged fermentation of feather residue as substrate. collagenase showed 2.09-fold increase specific activity 22.85% yield. monomeric protein with molecular mass 28.7 kDa, estimated SDS-PAGE AKTA system. optimum temperature pH for were around 40°C 8.0,...
This study investigated the balance between treatment efficiency and impact caused by urban wastewater plants (UWTPs) on dissemination of antibiotic resistance. Four full-scale UWTPs (PT1-PT4) receiving river were sampled over four campaigns. The 16 S rRNA gene, two mobile genetic elements (MGEs), eight resistance genes (ARGs), culturable bacteria monitored different stages in hospital effluent. bacterial load was not significantly inflow (p > 0.01). Biological promoted ARGs reduction values...
Carbapenem-resistant Klebsiella pneumoniae represents a healthcare threat, already disseminated in the environment. This study aimed to compare behaviour of clinical and an environmental K. strain (multilocus sequence type ST147) harbouring gene blaKPC-3 water. The abundance genes phoE (specific for pneumoniae) was monitored by quantitative PCR urban runoff water sterile ultra-pure microcosms, aiming assess survival, persistence, effect native microbiota. In water, cultivable did not change...
Chronic Kidney Disease (CKD) is a growing public-health concern worldwide. Patients exhibit compromised immunity and are more prone to infection than other populations. Therefore, oral colonization by clinically relevant members of the Enterobacteriaceae family, major agents both nosocomial dialysis-associated infections with frequent prevalence antibiotic resistances, may constitute serious risk. Thus, this study aimed assess occurrence enterobacteria their resistance profiles in cavity CKD...
In addition to environmental pollution issues, social concerns about the sustainability, safety, and quality of conventionally grown fruits vegetables have been increasing. order evaluate if there were any microbiological differences between samples organic conventional lettuce, a wide range parameters tested, including pathogens indicator organisms: enumeration Escherichia coli; detection Salmonella spp.; detection/enumeration Listeria monocytogenes; lactic acid bacteria, Pseudomonas spp....
Carbapenem-resistant Klebsiella pneumoniae are a major global threat in healthcare facilities. The propagation of carbapenem resistance determinants can occur through vertical transmission, with genetic elements being transmitted by the host bacterium, or horizontal same transferred among distinct bacterial hosts. This work aimed to track transmission K . facility. study involved polyphasic approach based on conjugation assays, phenotype and genotype analyses, whole genome sequencing,...
<b>Introduction:</b> Bronchiectasis exacerbations are often treated with broad spectrum antibiotics and patients thus at risk of acquiring multidrug resistant (MDR) bacteria. The aim was to identify factors associated isolation MDR pathogens in bronchiectasis exacerbations. <b>Methods:</b> Retrospective observational study hospitalized patients. Data compared between two groups according <b>Results:</b> sample included 84 a mean age 75,6. bacteria were isolated 28,6% the being <i>Pseudomonas...
Asthma is a chronic disease with clinical variations throughout its course. Periods of symptom aggravation can occur the need to escalate therapy. Nevertheless, further investigation should be considered when atypical manifestations appear or worsening symptoms occurs. Proximal tracheal/bronchial obstruction mimic observed in patients asthma. Primary benign tracheal tumors are rare, but constitute cause for such symptoms. We present case report patient asthma and lesion.
<b>Introduction:</b> Non-cystic fibrosis bronchiectasis (NCFB) is a multidimensional and etiologically diverse disease and, therefore, no single parameter can be used to determine its overall severity prognosis. In this regard, two different validated scores are currently for such assessment: the FACED score BSI. <b>Objective:</b> To compare results of assessment NCFB obtained via BSI scores. <b>Methods:</b> A retrospective study including patients from sample attending Functional Breathing...
Over the years medical imaging has been developed based on interaction between two different requirements. The first is demand from clinicians to increase accuracy in their diagnostic. second relationship basic, translational, clinical researchers and biomedical engineers committed research development of new diagnostic techniques. In this paper we report application analysis techniques quantification [ <sup xmlns:mml="http://www.w3.org/1998/Math/MathML"...