A5026705198- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Ovarian cancer diagnosis and treatment
- Circular RNAs in diseases
- Endometriosis Research and Treatment
- Telomeres, Telomerase, and Senescence
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Congenital heart defects research
- Liver Disease Diagnosis and Treatment
- Reproductive Biology and Fertility
- Endometrial and Cervical Cancer Treatments
- Planarian Biology and Electrostimulation
- Caveolin-1 and cellular processes
- Diet and metabolism studies
- Nuclear Structure and Function
- Thyroid Disorders and Treatments
- Uterine Myomas and Treatments
- Digestive system and related health
- Muscle Physiology and Disorders
- Pregnancy and preeclampsia studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Intraperitoneal and Appendiceal Malignancies
Carol Davila University of Medicine and Pharmacy
2016-2025
Institutul National pentru Sanatatea Mamei si Copilului "Alessandrescu-Rusescu"
2017-2025
Institutul National Victor Babes
2006-2021
Bukovinian State Medical University
2021
Iuliu Hațieganu University of Medicine and Pharmacy
2021
Academy of Romanian Scientists
2020
Henri Coanda Air Force Academy
2016
Military Technical Academy
2016
Clinical Emergency Hospital Bucharest
2009-2012
National Institute of Pathology
2012
AIM:To investigate the diagnostic accuracy of acoustic radiation force impulse (ARFI) imaging as a noninvasive method for assessment liver fibrosis in chronic hepatitis C (CHC) patients. METHODS:We performed prospective blind comparison ARFI elastography, APRI index and FibroMax consecutive series patients who underwent biopsy CHC University Hospital Bucharest.Histopathological staging according to METAVIR scoring system served reference.A total 74 index, successful biopsy. RESULTS:The tests...
We show here (presumably for the first time) a special type of cell in human and rat exocrine pancreas. These cells have phenotypic characteristics enteric interstitial Cajal (ICC). To identify pancreatic (pICC) we used routine light microscopy, non-conventional microscopy (less than 1 μm semi-thin sections Epon-embedded specimens cut by ultramicrotomy stained with Toluidine blue), transmission electron (TEM), immunocytochemistry. The results showed that pICC can be recognized easily...
Conventionally, cells described in the stroma of intestinal wall are fibroblasts/fibrocytes, mast cells, plasma eosinophils, macrophages and, interstitial Cajal (ICCs), latter being considered as pacemakers gastrointestinal rhythmicity. Recently, a new type stromal cell called telocyte (TCs) was found various cavitary and non-cavitary organs (www.telocytes.com). We show here direct electron microscopical evidence for presence TCs lamina propria rat jejunum just beneath epithelial layer...
Telocytes (TCs) have been described in various organs and species (www.telocytes.com) as cells with telopodes (Tps) - very long cellular extensions an alternation of thin segments (podomers) dilated portions (podoms). We examined TCs using electron microscopy (EM), immunohistochemistry (IHC), immunofluorescence (IF), time-lapse videomicroscopy whole-cell patch voltage clamp. EM showed a three-dimensional network dichotomous-branching Tps, labyrinthine system homocellular heterocellular...
Dysregulation of microRNAs has been implicated in many cardiovascular diseases including fibrosis.Here we report that miR-433 was consistently elevated three models heart disease with prominent cardiac fibrosis, and enriched fibroblasts compared to cardiomyocytes.Forced expression neonatal rat increased proliferation their differentiation into myofibroblasts as determined by EdU incorporation, α-SMA staining, levels fibrosis-associated genes.Conversely, inhibition exhibited opposite...
Telocytes (TCs), a novel type of interstitial cells, were recently described in the space tissues (www.telocytes.com). TCs have several very long, moniliform extensions, namely telopodes (Tps). However, functional role(s) is not yet understood. Successive photomicrographs ultrathin sections concatenated to capture entire length Tps which usually measure tens hundreds micrometres. Besides podoms (dilations) and podomers (thin segments), ultrastructural features include dichotomous branching...
Exercise can stimulate physiological cardiac growth and provide cardioprotection effect in ischemia/reperfusion (I/R) injury. MiR-210 is regulated the adaptation process induced by exercise; however, its impact on exercise-induced contribution to exercise-driven remain unclear. We investigated role mechanism of miR-210 explored whether contributes protection alleviating I/R Here, we first observed that regular swimming exercise markedly increase levels heart blood samples rats mice....
We describe here - presumably for the first time-a Cajal-like type of tubal interstitial cells (t-ICC), resembling archetypal enteric ICC. t-ICC were demonstrated in situ and vitro on fresh preparations (tissue cryosections primary cell cultures) using methylene-blue, crystal-violet, Janus-Green B or Mito Tracker-Green FM Probe vital stainings. Also, identified fixed specimens by light microscopy (methylene-blue, Giemsa, trichrome stainings, Gomori silver-impregnation) transmission electron...
Previous reports describing Cajal-like interstitial cells in human uterus are contradictory terms of c-kit immunoreactivity: either negative (but vimentin-positive) pregnant myometrium, or positive, presumably the endometrium. The aim this study was to verify existence myometrial (m-CLIC). Six different, complementary approaches were used: 1) methylene-blue supravital staining tissue samples (cryosections), 2) methylene blue and Janus green B vital (m-CLIC mitochondrial markers,...
We present transmission electron microscope (TEM) evidence that ICC and ICC-like cells frequently establish close contacts (synapses) with several types of immunoreactive (IRC): lymphocytes, plasma cells, eosinophils, basophils, macrophages mast cells. Such synapses were found in various organs: human mammary gland myometrium, as well rat stomach, gut, bladder uterus. Specimens observed by conventional TEM on ultrathin sections. Based morphometric analyses computer-aided 3-D reconstructions...
Abstract Telocytes ( TC s) were recently described as interstitial cells with very long prolongations named telopodes (Tps; www.telocytes.com ). Establishing the proteome is a priority to show that s are distinct type of cells. Therefore, we examined molecular aspects lung by comparison fibroblasts FB s). Proteins extracted from primary cultures these analysed automated 2‐dimensional nano‐electrospray ionization liquid chromatography tandem mass spectrometry (2D Nano‐ ESI LC ‐ MS /...
Abstract We have shown in 2012 the existence of telocytes ( TC s) human dermis. s were described by transmission electron microscopy TEM ) as interstitial cells located non‐epithelial spaces (stroma) many organs (see www.telocytes.com ). very long prolongations (tens to hundreds micrometers) named Telopodes (Tps). These Tps a special conformation with dilated portions podoms (containing mitochondria, endoplasmic reticulum and caveolae) thin segments (below resolving power light microscopy),...
Abstract Telocyte ( TC ) is a newly identified type of cell in the cardiac interstitium www.telocytes.com ). s are described by classical transmission electron microscopy as cells with very thin and long telopodes (Tps; cellular prolongations) having podoms (dilations) podomers (very segments). s' three‐dimensional (3D) morphology still unknown. Cardiac seem to be particularly involved short distance intercellular signalling and, therefore, their 3D architecture important for understanding...
Telocytes (TCs) are described as a particular type of cells the interstitial space (www.telocytes.com). Their main characteristics very long telopodes with alternating podoms and podomers. Recently, we performed comparative proteomic analysis human lung TCs fibroblasts, demonstrating that clearly distinct cell type. Therefore, present study aims to reinforce this idea by comparing endothelial (ECs), since ECs share immunopositivity for CD34. We applied isobaric tag relative absolute...
Recently, telocytes (TCs) were described as a new cell type in the interstitial space of many organs, including myometrium. TCs are cells with very long, distinctive extensions named telopodes (Tps). It is suggested that play major role intercellular signaling, well morphogenesis, especially morphogenetic bioelectrical signaling. However, TC plasma membrane yet unexplored regarding presence and activity ion channels pumps. Here, we used combination vitro immunofluorescence patch-clamp...