Nathan R. Tucker
A5057301426- Congenital heart defects research
- Cardiomyopathy and Myosin Studies
- Atrial Fibrillation Management and Outcomes
- Cancer-related gene regulation
- RNA Research and Splicing
- Cardiac Valve Diseases and Treatments
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Function and Risk Factors
- Single-cell and spatial transcriptomics
- Genetic Associations and Epidemiology
- RNA modifications and cancer
- Congenital Heart Disease Studies
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Cardiac Fibrosis and Remodeling
- Renal and Vascular Pathologies
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Cardiovascular Effects of Exercise
- Long-Term Effects of COVID-19
- Genetic Mapping and Diversity in Plants and Animals
- Transplantation: Methods and Outcomes
SUNY Upstate Medical University
2024-2025
Masonic Medical Research Laboratory
2020-2024
Memorial University of Newfoundland
2021-2024
Medical Research Institute
2022-2023
Broad Institute
2016-2022
Massachusetts General Hospital
2013-2022
Boston University
2017-2020
Oncode Institute
2020
University Medical Center Utrecht
2017-2020
Harvard University
2014-2019
The human heart requires a complex ensemble of specialized cell types to perform its essential function. A greater knowledge the intricate cellular milieu is critical increase our understanding cardiac homeostasis and pathology. As recent advances in low-input RNA sequencing have allowed definitions transcriptomes at single-cell resolution scale, we applied these approaches assess transcriptional diversity nonfailing heart.
ABSTRACT The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, creates an urgent need for identifying molecular mechanisms that mediate viral entry, propagation, and tissue pathology. Cell membrane bound angiotensin-converting enzyme 2 (ACE2) associated proteases, transmembrane protease serine (TMPRSS2) Cathepsin L (CTSL), were previously identified as mediators of SARS-CoV2 cellular entry. Here, we assess cell type-specific RNA expression ACE2 , TMPRSS2 CTSL through integrated...
<h3>Importance</h3> Atrial fibrillation (AF) is the most common arrhythmia affecting 1% of population. Young individuals with AF have a strong genetic association disease, but mechanisms remain incompletely understood. <h3>Objective</h3> To perform large-scale whole-genome sequencing to identify variants related AF. <h3>Design, Setting, and Participants</h3> The National Heart, Lung, Blood Institute's Trans-Omics for Precision Medicine Program includes longitudinal cohort studies that...
Fibromuscular dysplasia (FMD) is a nonatherosclerotic vascular disease leading to stenosis, dissection and aneurysm affecting mainly the renal cerebrovascular arteries. FMD often an underdiagnosed cause of hypertension stroke, has higher prevalence in females (~80%) but its pathophysiology unclear. We analyzed ~26K common variants (MAF>0.05) generated by exome-chip arrays 249 patients 689 controls. replicated 13 loci (P<10−4) 402 cases 2,537 controls confirmed association between variant...
Genetic variants identified by genome-wide association studies explain only a modest proportion of heritability, suggesting that meaningful associations lie 'hidden' below current thresholds. Here, we integrate information from with epigenomic maps to demonstrate enhancers significantly overlap known loci associated the cardiac QT interval and QRS duration. We apply functional criteria identify do not meet significance are missed existing studies. these 'sub-threshold' signals represent...
Restrictive cardiomyopathy (RCM) is a rare characterized by impaired diastolic ventricular function resulting in poor clinical prognosis. Rarely, heritable forms of RCM have been reported, and mutations underlying identified genes that govern the contractile cardiomyocytes.We evaluated 8 family members across 4 generations history, physical examination, electrocardiography, echocardiography. Affected individuals presented with pleitropic syndrome progressive RCM, atrioventricular septal...
ZFHX3 (zinc finger homeobox 3), a gene that encodes large transcription factor, is at the second-most significantly associated locus with atrial fibrillation (AF), but its function in heart unknown. This study aims to identify causative genetic variation related AF and examine impact of Zfhx3 loss on cardiac mice. CRISPR-Cas9 genome editing, chromatin immunoprecipitation, luciferase assays pluripotent stem cell-derived cardiomyocytes were used locus. Cardiac was assessed by echocardiography,...
BackgroundA recent genome-wide association study identified a susceptibility locus for atrial fibrillation at the KCNN3 gene. Since gene encodes small conductance calcium-activated potassium channel, we hypothesized that overexpression of SK3 channel increases to cardiac arrhythmias.