A5049031951- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Plant Stress Responses and Tolerance
- Receptor Mechanisms and Signaling
- Plant Molecular Biology Research
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Plant and Biological Electrophysiology Studies
- Photosynthetic Processes and Mechanisms
- Influenza Virus Research Studies
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Biofuel production and bioconversion
- Tissue Engineering and Regenerative Medicine
- Orthopaedic implants and arthroplasty
- Mesenchymal stem cell research
- Plant Micronutrient Interactions and Effects
- Hepatitis C virus research
- Lipid Membrane Structure and Behavior
- Ion Transport and Channel Regulation
- Autophagy in Disease and Therapy
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood transfusion and management
Uniformed Services University of the Health Sciences
2021-2025
George Mason University
2025
Henry M. Jackson Foundation
2020-2023
Massachusetts General Hospital
2018-2023
Institut des Sciences des Plantes de Montpellier
2012-2021
Institut Agro Montpellier
2014-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Centre National de la Recherche Scientifique
2015-2021
L'Institut Agro
2021
Jackson Foundation
2021
Nitrogen and phosphorus are among the most widely used fertilizers worldwide. Nitrate (NO3−) phosphate (PO43−) also signalling molecules whose respective transduction pathways being intensively studied. However, plants continuously challenged with combined nutritional deficiencies, yet very little is known about how these integrated. Here we report identification of a highly NO3−-inducible NRT1.1-controlled GARP transcription factor, HRS1, document its genome-wide transcriptional targets,...
Abstract Ca2 +-dependent protein kinases (CPKs) form a large family of 34 genes in Arabidopsis (Arabidopsis thaliana). Based on their dependence Ca2+, CPKs can be sorted into three types: strictly Ca2+-dependent CPKs, Ca2+-stimulated (with significant basal activity the absence Ca2+), and essentially calcium-insensitive CPKs. Here, we report third type CPK, CPK13, which is expressed guard cells but whose role still unknown. We confirm expression CPK13 cells, show that its overexpression...
Calcium (Ca(2+)) is a second messenger involved in many plant signaling processes. Biotic and abiotic stimuli induce Ca(2+) signals within cells, which, when decoded, enable these cells to adapt response environmental stresses. Multiple examples of from plants containing the fluorescent yellow cameleon sensor (YC) have contributed definition signature some cell types such as root hairs, pollen tubes guard cells. YC is, however, limited use highly autofluorescent tissues, particular mesophyll...
Much remains to be learned about the molecular mechanisms underlying a class of human disorders called actinopathies. These genetic are characterized by loss-of-function mutations in actin-associated proteins that affect immune cells, leading immunopathology. However, much how cytoskeletal dysregulation promotes immunological dysfunction. The current study reveals macrophage actin cytoskeleton responds LPS/IFNγ stimulation biphasic manner involves cellular contraction followed spreading....
ZFHX3 (zinc finger homeobox 3), a gene that encodes large transcription factor, is at the second-most significantly associated locus with atrial fibrillation (AF), but its function in heart unknown. This study aims to identify causative genetic variation related AF and examine impact of Zfhx3 loss on cardiac mice. CRISPR-Cas9 genome editing, chromatin immunoprecipitation, luciferase assays pluripotent stem cell-derived cardiomyocytes were used locus. Cardiac was assessed by echocardiography,...
Structural virology has emerged as the foundation for development of effective antiviral therapeutics. It is pivotal in providing crucial insights into three-dimensional frame viruses and viral proteins at atomic or near atomic-level resolution. Structure-based assessment components, including capsids, envelope proteins, replication machinery, host interaction interfaces, are instrumental unravelling multiplex mechanisms infection, replication, pathogenesis. The structural elucidation...
The p24-family member, TMED9, has recently emerged as a player in secretory pathway protein quality control (PQC) that influences the trafficking and degradation of misfolded proteins. Here, we show TMED9 plays central role PQC GPI-anchored proteins (GPI-APs). Typically, upon release from endoplasmic reticulum (ER)-resident chaperone calnexin, GPI-APs traffic to Golgi by an ER-export called Rapid ER stress-induced Export (RESET). From Golgi, they access plasma membrane where are rapidly...
Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used data normalization differ between tissue types pathological states. Systematically, we investigated stability nine HKGs (Actb, B2m, Gapdh, Hprt1, Pgk1, Rplp0, Rplp2, Tbp, Tfrc) from tissues prone to remote organ dysfunction (lung, liver, kidney, muscle) following extremity trauma....
Abstract Statins are prescribed for prevention and treatment of coronary artery disease. have different cholesterol lowering abilities, with rosuvastatin atorvastatin being the most effective, while statins like simvastatin fluvastatin having lower effectiveness. Statins, in addition to their effects, can prevent isoprenylation Rab-GTPase proteins, a protein family important regulation membrane-bound trafficking. Here we show that endosomal localization Rab-GTPases (Rab5, Rab7 Rab11) was...
Abstract Background Transdermal osseointegrated prosthesis have relatively high infection rates leading to implant revision or failure. A principle cause for this complication is the absence of a durable impervious biomechanical seal at interface hard structure (implant) and adjacent soft tissues. This study explores possibility recapitulating an analogous cellular musculoskeletal-connective tissue interface, which present naturally occurring integumentary tissues where exits skin, such as...
Abstract The p24-family member, TMED9, has recently emerged as a player in secretory pathway protein quality control (PQC) that influences the trafficking and degradation of misfolded proteins. Here we show TMED9 plays central role PQC GPI-anchored proteins (GPI-APs). Typically, upon release from endoplasmic reticulum (ER)-resident chaperone calnexin, GPI-APs traffic to Golgi by an ER-export called Rapid ER stress-induced Export (RESET). From Golgi, they access plasma membrane where are...
Post-translational regulations of Shaker-like voltage-gated K+ channels were reported to be essential for rapid responses environmental stresses in plants. In particular, it has been shown that calcium-dependent protein kinases (CPKs) regulate Shaker Here, the focus was on KAT2, a channel cloned model plant Arabidopsis thaliana, where is expressed namely vascular tissues leaves. After co-expression KAT2 with AtCPK6 Xenopuslaevis oocytes, voltage-clamp recordings demonstrated stimulates...