A5062018843- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Trauma and Emergency Care Studies
- Heterotopic Ossification and Related Conditions
- Traumatic Brain Injury and Neurovascular Disturbances
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Orthopedic Infections and Treatments
- TGF-β signaling in diseases
- Sports injuries and prevention
- Traumatic Brain Injury Research
- Blood disorders and treatments
- Orthopaedic implants and arthroplasty
- Anesthesia and Neurotoxicity Research
- Molecular Biology Techniques and Applications
- Blood transfusion and management
- Extracellular vesicles in disease
- Diabetic Foot Ulcer Assessment and Management
- S100 Proteins and Annexins
- Bone fractures and treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive biology and impacts on aquatic species
- Pelvic and Acetabular Injuries
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Retinal Diseases and Treatments
Henry M. Jackson Foundation
2021-2024
Uniformed Services University of the Health Sciences
2020-2024
American University
2020-2022
Ischemia reperfusion (IR) injury results in devastating skeletal muscle fibrosis. Here, we recapitulate this with a mouse model of hindlimb IR which leads to Injury resulted extensive immune infiltration robust neutrophil extracellular trap (NET) formation the muscle, however, direct targeting NETs via peptidylarginine deiminase 4 (PAD4) mechanism was insufficient reduce Circulating levels IL-10 and TNFα were significantly elevated post injury, indicating toll-like receptor (TLR) signaling...
We describe a visual stimulus that can be used with both larval and adult zebrafish (Danio rerio). This protocol is modification of standard behavior analysis, the optomotor response (OMR). The OMR often to determine spatial or detect directional visuomotor deficiencies. An generated using high contrast grated pattern, typically vertical bars. sensitivity measured by detection change in grating bar width reported cycles per degree (CPD). test has been extensively larvae adults identify...
Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used data normalization differ between tissue types pathological states. Systematically, we investigated stability nine HKGs (Actb, B2m, Gapdh, Hprt1, Pgk1, Rplp0, Rplp2, Tbp, Tfrc) from tissues prone to remote organ dysfunction (lung, liver, kidney, muscle) following extremity trauma....
Trauma can result in systemic inflammation that leads to organ dysfunction, but the impact on brain, particularly following extracranial insults, has been largely overlooked.
Prolonged hyperglycemia causes long-term vision complications and an increased risk of cognitive deficits. High blood sugar also confers osmotic load/stress to cells. We assessed behavioral neurochemical changes in zebrafish brain retina following prolonged for 4-weeks or 8-weeks. At each time point, behavior was using 3-chamber choice task optomotor response; tissue then collected levels inflammatory markers, tight junction proteins, neurotransmitters determined Western Blots. After...
Neurodegenerative diseases are age-dependent, debilitating, and incurable. Recent reports have also correlated hyperglycemia with changes in memory and/or cognitive impairment. We modified developed a three-chamber choice task similar to that used rodents for use hyperglycemic zebrafish. The testing chamber consists of centrally located starting two compartments on either side, shoal conspecifics as the reward. provide data showing once acquired, zebrafish remember at least 8 weeks later....
Zebrafish (Danio rerio) are an excellent model to investigate the effects of chronic hyperglycemia, a hallmark Type II Diabetes Mellitus (T2DM). This alternate immersion protocol is noninvasive, step-wise method inducing hyperglycemia for up eight weeks. Adult zebrafish alternately exposed sugar (glucose) and water 24 hours each. The begin treatment in 1% glucose solution 2 weeks, then 2% finally 3% remaining 4 Compared water-treated (stress) mannitol-treated (osmotic) controls,...
Introduction Heterotopic ossification (HO) is a complex pathology often observed in combat injured casualties who have sustained severe, high energy polytraumatic extremity injuries. Once HO has developed, prophylactic therapies are limited outside of surgical excision. Tourniquet-induced ischemia injury (IR) exacerbates trauma-mediated musculoskeletal tissue injury, inflammation, osteogenic progenitor cell development and formation. Others shown that focal adhesion kinase-2 (FAK2) plays key...
Neurodegenerative diseases are age-dependent, debilitating, and incurable. Recent reports have also correlated hyperglycemia with changes in memory and/or cognitive impairment. We modified developed a three-chamber choice task similar to that used rodents for use hyperglycemic zebrafish. The testing chamber consists of centrally located starting two compartments on either side, shoal conspecifics as the reward. provide data showing once acquired, zebrafish remember at least 8 weeks later....
Zebrafish (Danio rerio) are an excellent model to investigate the effects of chronic hyperglycemia, a hallmark Type II Diabetes Mellitus (T2DM). This alternate immersion protocol is noninvasive, step-wise method inducing hyperglycemia for up eight weeks. Adult zebrafish alternately exposed sugar (glucose) and water 24 hours each. The begin treatment in 1% glucose solution 2 weeks, then 2% finally 3% remaining 4 Compared water-treated (stress) mannitol-treated (osmotic) controls,...
Purpose: Muscle fibrosis is a devastating condition that can occur following traumatic injury leading to loss of function and poor outcomes. Limited noninvasive modalities exist diagnose characterize the extent muscle damage. Here we investigate spectral ultrasound imaging (SUSI), quantitative technique which detects tissue parameters based on intrinsic structure composition, identify early fibrotic changes established in rat model. We hypothesize SUSI will detect clinically translational...