A5085838031- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Genetic Neurodegenerative Diseases
- RNA Interference and Gene Delivery
- Erythropoietin and Anemia Treatment
- Alzheimer's disease research and treatments
- Nerve injury and regeneration
- Virus-based gene therapy research
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Child and Adolescent Health
- Biochemical effects in animals
- Neurological Disorders and Treatments
- Neurological Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Barrier Structure and Function Studies
- CAR-T cell therapy research
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- Cancer Cells and Metastasis
- Migration, Health and Trauma
University of Tübingen
2013-2024
Yerevan State Medical University
2012-2024
University Children's Hospital Tübingen
2009-2023
National Association of Statutory Health Insurance Physicians
2021
American University of Armenia
2012
Columbia University
2012
University of Massachusetts Boston
2012
Northeastern University
2012
TH Bingen University of Applied Sciences
2009
Safe and effective cell delivery remains one of the main challenges in cell-based therapy neurodegenerative disorders. Graft survival, sufficient enrichment therapeutic cells brain, avoidance their distribution throughout peripheral organs are greatly influenced by method delivery. Here we demonstrate for first time noninvasive intranasal (IN) mesenchymal stem (MSCs) to brains unilaterally 6-hydroxydopamine (6-OHDA)-lesioned rats. IN application (INA) MSCs resulted appearance olfactory bulb,...
The local renin–angiotensin system (RAS) in the brain is a multitasking controlling plethora of essential functions such as neurogenic hypertension, baroreflexes, and sympathetic activity. Aside from its vasoactive actions, angiotensin II (AT-II) has also been implicated pathogenesis cognitive decline, beneficial effects receptor blockers (ARBs) Alzheimer (AD) Parkinson diseases (PD) are suggested. However, use ARBs at antihypertensive dosages would lead to unwanted hypotensive reactions AD...
In view of the rapid preclinical development cell-based therapies for neurodegenerative disorders, traumatic brain injury, and tumors, safe efficient delivery targeting therapeutic cells to central nervous system is critical maintaining efficacy safety in respective disease models. Our previous data demonstrated therapeutically efficacious targeted mesenchymal stem (MSCs) rat 6-hydroxydopamine model Parkinson's (PD). The present study examined bone marrow-derived MSCs, macrophages, microglia...
Intrastriatal administration of mesenchymal stem cells (MSCs) has shown beneficial effects in rodent models Huntington disease (HD). However, the invasive nature surgical procedure and its potential to trigger host immune response may limit clinical use. Hence, we sought evaluate non-invasive intranasal (INA) MSC delivery as an effective alternative route HD. GFP-expressing MSCs derived from bone marrow were intranasally administered 4-week-old R6/2 HD transgenic mice. detected olfactory...
Locus coeruleus-noradrenergic system dysfunction is known to contribute the progression of Alzheimer’s disease (AD). Besides a variety reports showing involvement norepinephrine and its receptor systems in cognition, amyloid β (Aβ) metabolism, neuroinflammation, neurogenesis, little about contribution specific receptors these actions. Here, we investigated neurogenic neuroprotective properties new α2 adrenoblocker, mesedin, astroglial primary cultures (APC) from C57BL/6 3×Tg-AD mice. Our...
Stem cells` (SC) functional heterogeneity and its poorly understood aetiology impedes clinical development of cell-based therapies in regenerative medicine oncology. Recent studies suggest a strong correlation between the SC migration potential their therapeutic efficacy humans. Designating as denominator heterogeneity, we sought to identify highly migrating subpopulations within different classes evaluate properties comparison parental non-selected cells.We selected from mesenchymal neural...
Angiotensin receptor blockers (ARBs) have demonstrated multiple neuroprotective benefits in Alzheimer’s disease (AD) models. However, their beneficial effects on memory deficits, cholinergic activity, neurogenesis and Amyloid beta (Aβ) clearance reveal significant interstudy variability. The delivery route can impact not only but also targeting therapeutic efficacy of ARBs. Our previous findings the intranasally delivered losartan APP/PS1 model AD prompted us to explore influence by...
Abstract The hepatic content of amyloid beta (Aβ) decreases drastically in human and rodent cirrhosis highlighting the importance understanding consequences Aβ deficiency liver. This is especially relevant view recent advances anti‐Aβ therapies for Alzheimer's disease (AD). Here, it shown that partial loss transgenic AD mice immunized with antibody 3D6 its absence precursor protein (APP) knockout (APP‐KO), as well liver spheroids APP knockdown upregulates classical hallmarks fibrosis, smooth...
Extracellular accumulation of toxic concentrations glutamate (Glu) is a hallmark many neurodegenerative diseases, often accompanied by hypoxia and impaired metabolism this neuromediator. To address the question whether multifunctional neuroprotective action erythropoietin (EPO) extends to regulation extracellular Glu-level age-related, young culture-aged rat astroglial primary cells (APC) were simultaneously treated with 1mM Glu and/or human recombinant EPO under normoxic hypoxic conditions...
Summary Incompatible blood group antigens are highly immunogenic and can cause graft rejections. Focusing on distinct carbohydrate‐ protein‐based membrane structures, defined by antigens, we investigated human bone marrow‐derived mesenchymal stem cells (MSCs) cultured in serum. The presence of H (CD173), ABO, RhD, RhCE, RhAG, Kell, urea transporter type B (SLC14A1, previously known as JK), Duffy antigen receptor chemokines (DARC) was evaluated at the levels genome, transcriptome antigen....
BackgroundLimited knowledge of stem cell therapies` mechanisms action hampers their sustainable implementation into the clinic. Specifically, interactions transplanted cells with host vasculature and its implications for therapeutic efficacy are not elucidated. We tested whether adhesion receptors chemokine on can be functionally modulated, consequently if such modulation may substantially affect therapeutically relevant endothelium.MethodsWe investigated effects cationic molecule...
The noradrenergic system is proposed to play a prominent role in the pathogenesis of liver fibrosis. While α1- and β-adrenergic receptors (ARs) are suggested be involved multitude profibrogenic actions, little known about α2-AR-mediated effects their expression pattern during fibrosis cirrhosis. We explored α2-AR two models experimental further evaluated capacity blocker mesedin deactivate hepatic stellate cells (HSCs) increase permeability human sinusoidal endothelial (hLSECs). mRNA α2a-,...
A glioblastoma (GBM) is an aggressive and lethal primary brain tumor with restricted treatment options a dismal prognosis. Oncolytic virotherapy (OVT) has developed as promising approach for GBM treatment. However, reaching invasive cells may be hindered by tumor-surrounding, non-neoplastic when the oncolytic virus (OV) applied intratumorally. Using two xenograft mouse models immunofluorescence analyses, we investigated intranasal delivery of adenovirus (OAV) XVir-N-31 via virus-loaded,...
Amyloid beta (Aβ) plays a major role in the pathogenesis of Alzheimer’s disease and, more recently, has been shown to protect against liver fibrosis. Therefore, we studied Aβ-42 levels and expression genes involved generation, degradation, transport Aβ proteins samples from patients at different stages metabolic dysfunction-associated (MASLD) under steatotic conditions vitro/in vivo. precursor protein (APP), key Aβ-metabolizing proteins, were analyzed using RT-PCR, Western blotting, Luminex...
In inner organs, glutamine contributes to proliferation, detoxification and establishment of a mechanical barrier, i.e., functions essential for skin, as well. However, the age-dependent regional peculiarities distribution synthetase (GS), an enzyme responsible generation glutamine, factors regulating its enzymatic activity in mammalian skin remain undisclosed. To explore this, GS localization was investigated using immunohistochemistry double-labeling young adult human rat sections well...
The function and regulation of amyloid-beta (Aβ) in healthy diseased liver remains unexplored. Because Aβ reduces the integrity blood-brain barrier we have examined its potential role regulating sinusoidal permeability normal cirrhotic liver. key proteins that generate (beta-secretase 1 presenilin-1) degrade it (neprilysin myelin basic protein) were decreased human In culture, activated hepatic stellate cells (HSC) internalized more efficiently than astrocytes HSC degraded leading to...
A number of the QL researches in case different pathologies are being increased during last decade. The existing traditional research methods provide mostly arbitrary data on disease and its treatment, which not sufficient for schoolchildren overall psychological social adaptation wellness evaluation.The object became 3 randomly selected schools Yerevan. 443 monitoring units formed selection population. degree situational personal anxiety was evaluated with help Spielberger's Gerbachevski's...
Amyloid-beta (Aβ) deposition in the brain is main pathological hallmark of Alzheimer disease. Peripheral clearance Aβ may possibly also lower levels. Recent evidence suggested that hepatic Aβ42 impaired liver cirrhosis. To further test this hypothesis, serum was measured by ELISA portal venous (PVS), systemic (SVS), and (HVS) 20 patients with Mean level 24.7 ± 20.4 pg/mL PVS, 21.2 16.7 HVS, 19.2 11.7 SVS. Similar levels three blood compartments cirrhotic does not clear Aβ42. neither...