A5003575996- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Microscopic Colitis
- Galectins and Cancer Biology
- Celiac Disease Research and Management
- Radiopharmaceutical Chemistry and Applications
- Carbohydrate Chemistry and Synthesis
- Inflammatory Bowel Disease
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Treatments and Studies
- Cell Adhesion Molecules Research
- Reproductive System and Pregnancy
- Molecular Biology Techniques and Applications
- Gastric Cancer Management and Outcomes
- Peptidase Inhibition and Analysis
- Escherichia coli research studies
- Genetic factors in colorectal cancer
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Chemokine receptors and signaling
- Antimicrobial Peptides and Activities
Umeå University
2014-2024
In-Q-Tel
2011
Pediatrics and Genetics
2004
Lawrence Livermore National Laboratory
1994
Stockholm University
1979-1988
Karolinska Institutet
1965-1983
Wenner-Gren Foundations
1967-1982
Karolinska University Hospital
1971-1981
Columbia University
1972
The Neurological Institute
1972
Abstract The accuracy of 18S rRNA, β‐actin mRNA and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) as indicators cell number when used for normalization in gene expression analysis T lymphocytes at different activation stages was investigated. Quantitative real‐time reverse transcriptase‐polymerase chain reaction to determine the level mRNA, GAPDH six cytokines carefully counted samples resting human peripheral blood mononuclear cells (PBMCs), intestinal PBMCs subjected polyclonal T‐cell...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and characterization of a blood-group A reactive hemagglutinin from the snail Helix pomatia study its combining siteSten Hammarstrom Elvin A. KabatCite this: Biochemistry 1969, 8, 7, 2696–2705Publication Date (Print):July 1, 1969Publication History Published online1 May 2002Published inissue 1 July 1969https://pubs.acs.org/doi/10.1021/bi00835a002https://doi.org/10.1021/bi00835a002research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbohydrate binding specificity of four N-acetyl-D-galactosamine-"specific" lectins: Helix pomatia A hemagglutinin, soy bean agglutinin, lima lectin, and Dolichos biflorus lectinSten Hammarstrom, Lee A. Murphy, Irwin J. Goldstein, Marilynn E. EtzlerCite this: Biochemistry 1977, 16, 12, 2750–2755Publication Date (Print):June 14, 1977Publication History Published online1 May 2002Published inissue 14 June...
SUMMARY The impact of chronic inflammation on the expression human α-defensins 5 and 6 (HD-5, HD-6), β-defensins 1 2 (hBD-1, hBD-2) lysozyme in epithelial cells small large intestine was investigated. Intestinal specimens from 16 patients with ulcerative colitis (UC), 14 Crohn's disease (CD) 40 controls no history inflammatory bowel were studied. mRNA levels five defence molecules determined freshly isolated by real-time quantitative RT-PCR. Specific copy standards used allowing comparison...
The human gut epithelium is a unique immunological compartment, containing substantial amounts of Intra-epithelial lymphocytes (IEL) with unknown functions. In this study we show that distinct and unusual subpopulations IEL are present at different levels Intestine. phenotypes normal jejunum, ileum colon were compared using Immunoflow cytometry immunohlstochemistry. expression mRNA for recombination-activating gene-1 (RAG-1) from all three was reverse-transcription polymerase chain reaction,...
OBJECTIVES Exposure to gliadin and related prolamins appropriate HLA-DQ haplotype are necessary but not sufficient for contracting celiac disease (CD). Aberrant innate immune reactions could be contributing risk factors. Therefore, jejunal biopsies were screened bacteria the status of epithelium investigated. METHODS Children with untreated, treated, challenged CD, controls analyzed. Bacteria identified by scanning electron microscopy. Glycocalyx composition mucin antimicrobial peptide...
Alterations in the composition of microbiota intestine may promote development celiac disease (CD). Using scanning electron microscopy (SEM) we previously demonstrated that rod-shaped bacteria were present on epithelium proximal small children with CD but not controls. In this study characterize and controls identify CD-associated bacteria.Proximal biopsies from 45 18 clinical studied. Bacteria identified by 16S rDNA sequencing DNA extracted washed buffer containing dithiothreitol to enrich...
The incidence and height of antibody titers to colon, assayed by indirect hemagglutination with a heat stable colon extract from germ free rats, is significantly higher in sera patients ulcerative colitis than those healthy controls or amebic liver abscess dysentery. While are indistinguishable regard Forsman antigen, Staphylococcus aureus S 209, Clostridium difficile, several common strains E. coli, they have elevated increased antibodies antigen coli O14. Patients dysentery normal such...
A new type of immunoassay (enzyme-linked immunosorbent assay, ELISA) has been used for the detection antibodies against Salmonella O antigens. The results show that ELISA is specific and highly sensitive. When compared with Widal reaction, passive hemagglutination, quantitative precipitation, was shown to be more In addition, immunoglobulin G M were both detected approximately as efficiently ELISA.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpecificity and binding properties of the blood group A reactive hemagglutinin from Helix pomatiaElvin A. Kabat Sten HammarstromCite this: Biochemistry 1971, 10, 9, 1684–1692Publication Date (Print):April 1, 1971Publication History Published online1 May 2002Published inissue 1 April 1971https://pubs.acs.org/doi/10.1021/bi00785a028https://doi.org/10.1021/bi00785a028research-articleACS PublicationsRequest reuse permissionsArticle...
Autoantibodies to germ free rat colon were produced in rats by repeated intraperitoneal injections with newborn rabbit Freund's complete adjuvant. Antibodies assayed means of indirect hemagglutination sheep erythrocytes sensitized a heat stable phenol-water extract or fluorescent antibody staining sections. The response injected was less intense. A few the adjuvant alone also developed an anti-colon titer. Hemagglutination inhibition experiments demonstrated that antibodies specific for...
SUMMARY Ulcerative colitis (UC), a chronic inflammatory bowel disease, exhibits pronounced increase of T lymphocytes in the inflamed mucosa. To understand role intestinal pathogenesis UC their cytokine production mucosa was analysed. Intestinal UC, Crohn's disease and control patients were analysed for mRNA levels by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) directly after isolation without vitro stimulation. Frequencies positive cells determined colon...
The functional properties of intraepithelial lymphocytes (IEL) in normal human jejunum, ileum, and colon were investigated. Cytokine mRNA expression IEL enterocytes was determined by reverse transcriptase-PCR IFN-gamma+ immunohistochemistry. Polyclonal activators used to study proliferation IFN-gamma secretion IEL, an anti-CD3-mediated redirected cytotoxicity assay determine the lytic potential IEL. Freshly isolated at all three gut levels expressed for IL-1 beta, IL-2, IL-8, IFN-gamma,...
The carbohydrate binding specificity of leukoagglutinin (La; Phaseolus vulgaris isolectin L4) was studied by using quantitative precipitation and precipitation-inhibition. A series purified glycopeptides synthetic oligosaccharides were used as inhibitors. minimal structural unit required for La the disaccharide GlcNac(1 leads to beta 2)Man. Additions this basic different sugar residues gave a positive or negative contribution binding. most complementary structure pentasaccharide (formula:...
SUMMARY mRNA expression of two recently described human β-defensins (hBD-3 and hBD-4) in epithelial cells normal small large intestine the impact chronic intestinal inflammation on their levels was investigated. Intestinal specimens from patients with ulcerative colitis (UC), Crohn's disease (CD) controls no history inflammatory bowel were studied. hBD-3 hBD-4 mRNAs determined freshly isolated by real-time quantitative reverse transcription–polymerase chain reaction (QRT-PCR) situ...
We have shown that gamma delta T cells in human gingiva an intraepithelial location and, the chronic inflammatory disease periodontitis, expression of CD45RO and CD8 or CD4 is induced on cells. To study role local antibacterial responses, we determined cytokine profiles isolated gingival Different cell subpopulations, by positive selection with mAb-coated magnetic beads macrophages, as well epithelial cells, were analyzed for mRNA 15 cytokines reverse transcriptase-PCR. The ultrastructure...