A5054061366- Biomarkers in Disease Mechanisms
- Pancreatic and Hepatic Oncology Research
- Inflammasome and immune disorders
- Drug Transport and Resistance Mechanisms
- Polymer Surface Interaction Studies
- Epigenetics and DNA Methylation
- Molecular Junctions and Nanostructures
- MicroRNA in disease regulation
- RNA modifications and cancer
- Dendrimers and Hyperbranched Polymers
- Kruppel-like factors research
- Genetic Syndromes and Imprinting
- IL-33, ST2, and ILC Pathways
- Peptidase Inhibition and Analysis
- Genetic factors in colorectal cancer
- Blood groups and transfusion
- Leptospirosis research and findings
- Cancer-related gene regulation
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic function and diabetes
- Angiogenesis and VEGF in Cancer
- Psoriasis: Treatment and Pathogenesis
- Cancer Diagnosis and Treatment
- Gastrointestinal Tumor Research and Treatment
- Dermatology and Skin Diseases
Nippon Medical School
2019-2021
The University of Tokyo
2006-2017
Humanitas University
2014-2016
IRCCS Humanitas Research Hospital
2015
Fondazione Humanitas per la Ricerca
2013
Osaka City University
2009
Hepatocyte nuclear factor-4α (HNF4α) exists in multiple isoforms that are generated by alternative promoter (P1 and P2) usage splicing. Here we establish monoclonal antibodies (mAbs) for detecting P1 P2 promoter-driven HNF4α, evaluate their expression normal adult human tissues surgically resected carcinomas of different origins. Using immunohistochemical analysis, demonstrate that, while HNF4α is expressed hepatocytes, small intestine, colon, kidney epididymis, bile duct, pancreas, stomach,...
Abstract The physiological roles of the factor H (FH)-related proteins are controversial and poorly understood. Based on genetic studies, FH-related protein 5 (CFHR5) is implicated in glomerular diseases, such as atypical hemolytic uremic syndrome, dense deposit disease, CFHR5 nephropathy. was also identified immune deposits at level. For CFHR5, weak complement regulatory activity competition for C3b binding with plasma inhibitor FH have been reported, but its function remains elusive. In...
Cholesterol homeostasis is maintained by coordinate regulation of cholesterol synthesis and its conversion to bile acids in the liver. The excretion from liver intestine regulated ATP-binding cassette half-transporters ABCG5 ABCG8. genes for these two proteins are closely linked divergently transcribed a common intergenic promoter region. Here, we identified binding site hepatocyte nuclear factor 4alpha (HNF4alpha) ABCG5/ABCG8 promoter, through which HNF4alpha strongly activated expression...
Abstract Gastrointestinal adenocarcinomas (GIAC) of the tubular gastrointestinal (GI) tract including esophagus, stomach, colon, and rectum comprise most GI cancers share a spectrum genomic features. However, unified epigenomic changes specific to GIAC are poorly characterized. Using 907 samples from The Cancer Genome Atlas, we applied mathematical algorithms large-scale DNA methylome transcriptome profiles reconstruct transcription factor (TF) networks identify list functionally hyperactive...
Pentraxin 3 (PTX3), a long pentraxin subfamily member in the family, plays an important role innate immunity as soluble pattern recognition receptor. Plasma PTX3 is elevated sepsis (~200 ng/ml) and correlates with mortality. The roles of sepsis, however, are not well understood. To investigate ligands we performed targeted proteomic study circulating complexes using magnetic bead-based immunopurification shotgun proteomics for label-free relative quantitation via spectral counting. From...
Pentraxin 3 (PTX3) is a soluble pattern recognition receptor which classified as long-pentraxin in the pentraxin family. It known to play an important role innate immunity, inflammatory regulation and female fertility. PTX3 synthesized by specific cells, primarily response signals. Among these various neutrophils have unique production system. Neutrophils store neutrophil-specific granules then stored released localizes neutrophil extracellular traps (NETs). Although certain NET components...
Bundling extracellular histone proteins together blocks their cytotoxic effects and protects against sepsis.
Hepatocyte nuclear factor-4α (HNF4α, NR2A1) is a receptor that has critical role in hepatocyte differentiation and the maintenance of homeostasis adult liver. However, detailed understanding native HNF4α steady-state remains to be elucidated. Here we report isoform, phosphorylation status, complexes steady-state, as shown by shotgun proteomics HepG2 hepatocarcinoma cells. Shotgun proteomic analysis revealed complexity HNF4α, including multiple sites inter-isoform heterodimerization. The...
Type 1 iodothyronine deiodinase (Dio1), a selenoenzyme catalyzing the bioactivation of thyroid hormone, is highly expressed in liver. Dio1 mRNA and enzyme activity levels are markedly reduced livers hepatocyte nuclear factor 4alpha (HNF4alpha)-null mice, thus accounting for its liver-specific expression. Consistent with this deficiency, serum T4 rT3 concentrations elevated these mice compared those HNF4alpha-floxed control littermates; however, T3 unchanged. Promoter analysis mouse gene...
Kawasaki disease (KD) is a febrile of childhood characterized by systemic vasculitis that can lead to coronary artery lesions (CAL). This was prospective cohort study determine the levels pentraxin 3 (PTX3), soluble CD24-Subtype (Presepsin) and N-terminal pro-brain natriuretic peptide (NT-pro BNP) in consecutive KD patients. From January 2013 March 2015, all patients with admitted Aichi Medical University Hospital who provided consent had their plasma saved before IVIG administration. In...
Myosin is a functional protein associated with cellular movement, cell division, muscle contraction and other functions. Members of the myosin super-family are distinguished from heavy chains that play crucial roles in processes. Although there many studies this family, fewer on non-muscle than chains. classified as type I (myosin I) or II II). I, called unconventional mini-myosin, has one head, while II, conventional myosin, two heads. We transfected polypeptide 9 (MYH9) into HeLa cells...
Ribosomal RNA (rRNA), the most abundant non-coding species, is a major component of ribosome. Impaired ribosome biogenesis causes dysfunction protein synthesis and diseases called “ribosomopathies,” including genetic disorders with cancer risk. However, potential role rRNA gene (rDNA) alterations in unknown. We investigated germline somatic single-nucleotide variants (SNVs) rDNA promoter region (positions −248 to +100, relative transcription start site) 82 lung adenocarcinomas (LUAC)....
Abstract Backgrounds Gastrointestinal adenocarcinomas (GIACs) of the tubular GI tract including esophagus, stomach, colon and rectum comprise most cancers share a spectrum genomic features. However, unified epigenomic changes specific to GIACs are less well-characterized.We applied mathematical algorithms large-scale DNA methylome transcriptome profiles reconstruct transcription factor (TF) networks using 907 GIAC samples from The Cancer Genome Atlas (TCGA). Complementary technologies were...
VOLUME 286 (2011) PAGES 674–686 PAGE 684: In Fig. 5C, the primer set names (Proximal and Distal) were switched. The corrected figure is shown below.