A5031701013- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Microbial Inactivation Methods
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Metal complexes synthesis and properties
- Immune cells in cancer
- Liver physiology and pathology
- Single-cell and spatial transcriptomics
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microfluidic and Bio-sensing Technologies
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Magnetism in coordination complexes
- Wnt/β-catenin signaling in development and cancer
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Synthesis and biological activity
Huazhong Agricultural University
2019-2025
Hubei Provincial Center for Disease Control and Prevention
2024
National Institutes of Health
2010-2023
Center for Cancer Research
2010-2023
National Cancer Institute
2010-2023
MRC Laboratory of Molecular Biology
2011-2013
Cancer Genetics (United States)
2013
East China Normal University
2013
Human Computer Interaction (Switzerland)
2013
Frederick National Laboratory for Cancer Research
2013
Glypican-3 (GPC3) has emerged as a candidate therapeutic target in hepatocellular carcinoma (HCC), but the oncogenic role of GPC3 HCC is poorly understood. Here, we report human heavy-chain variable domain antibody, HN3, with high affinity ( K d = 0.6 nM) for cell-surface–associated molecules. The antibody recognized conformational epitope that requires both amino and carboxy terminal domains GPC3. HN3 inhibited proliferation GPC3-positive cells exhibited significant inhibition xenograft...
Wnt signaling is important for cancer pathogenesis and often up-regulated in hepatocellular carcinoma (HCC). Heparan sulfate proteoglycans (HSPGs) function as coreceptors or modulators of activation. Glypican-3 (GPC3) an HSPG that highly expressed HCC, where it can attract proteins to the cell surface promote proliferation. Thus, GPC3 has emerged a candidate therapeutic target liver cancer. While monoclonal antibodies are currently being evaluated preclinical clinical studies, none have...
Glypican-3 is a cell surface glycoprotein that associates with Wnt in liver cancer. We develop two antibodies targeting glypican-3, HN3 and YP7. The first antibody recognizes functional epitope inhibits signalling, whereas the second C-terminal but does not inhibit signalling. Both are fused to fragment of Pseudomonas exotoxin A (PE38) create immunotoxins. Interestingly, immunotoxin based on (HN3-PE38) has superior antitumor activity as compared YP7 (YP7-PE38) both vitro vivo. Intravenous...
Wnt signaling is one of the key regulators hepatocellular carcinoma (HCC) tumor progression. In addition to classical receptor frizzled (FZD), various coreceptors including heparan sulfate proteoglycans (HSPGs) are involved in activation. Glypican‐3 (GPC3) an HSPG that overexpressed HCC and functions as a coreceptor modulates cell proliferation. These features make GPC3 attractive target for liver cancer therapy. However, precise interaction how GPC3, Wnt, FZD cooperate with each other...
ABSTRACT Background Shark new antigen receptor variable domain (VNAR) antibodies can bind restricted epitopes that may be inaccessible to conventional antibodies. Methods Here, we developed a library construction method based on polymerase chain reaction (PCR)-Extension Assembly and Self-Ligation (named “EASeL”) construct large VNAR antibody with size of 1.2 × 1010 from six naïve adult nurse sharks (Ginglymostoma cirratum). Results The next-generation sequencing analysis 1.19 million...
Glypican-3 (GPC3) represents an attractive target for hepatocellular carcinoma (HCC) therapy because it is highly expressed in HCC but not adult normal tissue. Recently, high affinity anti-GPC3 antibodies have been developed; however, full may penetrate evenly into tumor parenchyma, reducing their effectiveness. In this study, we compared a whole IgG antibody, YP7, with human heavy chain HN3, regard to relative therapeutic effects. Both YP7 and HN3 bound GPC3-positive A431/G1 cells were...
Background Small cell lung cancer (SCLC) accounts for 15% of cancers, and the primary treatment this malignancy is chemotherapy radiotherapy. Delta-like 3 (DLL3) an attractive target SCLC immunotherapy since its expression highly restricted to with a neglectable appearance on normal adult tissues. In current study, we aimed explore efficacy DLL3-targeted via engagement T cell. Methods As proof concept, constructed bispecific antibody chimeric antigen receptor (CAR)-modified cells. vitro in...
Abstract Consumption of dietary fiber and anthocyanin has been linked to a lower incidence colorectal cancer (CRC). This study scrutinizes the potential antitumorigenic attributes black rice diet (BRD), abundantly rich in anthocyanin. Our results demonstrate notable effects mice on BRD, indicated by reduction both size number intestinal tumors consequent extension life span, compared control diet‐fed counterparts. Furthermore, fecal transplants from BRD‐fed germ‐free led decrease colonic...
Chimeric antigen receptor (CAR)-modified T cell therapy has achieved remarkable efficacy in treating hematological malignancies, but it confronts many challenges solid tumors, such as the immunosuppressive microenvironment of tumors. These factors reduce antitumor activity CAR-T cells clinical trials. Therefore, we used immunocytokine interleukin-12 (IL-12) to enhance therapy. In this study, engineered CAR-IL12R54 that targeted mesothelin (MSLN) and secreted a single-chain IL-12 fused scFv...
Current pancreatic cancer immunotherapy focused on alphabeta (αβ) T cells, either through CD3-engaged bispecific antibodies or CAR-T. Despite their promise, dose-limited toxicity (DLT) remains a challenge in clinical practice. In light of these concerns, there is growing interest exploring alternative cell types, natural killer (NKT) cells and gammadelta (γδ) that possess the capacity to lyse tumors while potentially offering safer therapeutic profile with fewer side effects. These present...
Background While the role of specific nutrients in cancer is established, associations between comprehensive dietary nutrient intake and presence remain underexplored. This cross-sectional study investigates global profiles relation to solid blood cancers. Methods A total 42,732 mobile adults from National Health Nutrition Examination Survey (NHANES, 2001–2023) were enrolled this study. The potential intakes 34 4 common trace components with investigated by weighted logistic regression...
Background: Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) are the two most common primary liver cancers, yet there have been no significant advances in effective therapeutics.Mesothelin has reported as a new therapeutic target various types of cancer.Here, we investigated expression mesothelin cancer its potential role novel for immunotherapy.Methods: HCC CCA specimens were examined by immunohistochemistry expression.Protein was assessed immunoblotting flow cytometry.The SS1P...
Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that highly expressed on the cell surface of mesothelioma, ovarian cancer and other malignant tumors. The interaction between mesothelin CA125 (also called MUC16) may facilitate implantation metastasis tumors in peritoneal cavity. A desirable therapeutic agent involves finding fully human monoclonal antibody (mAb) binds to or inhibits their interaction. Here, we report identification novel mAb mesothelin. HN1, single-chain Fv...
Monoclonal antibodies against mesothelin are being evaluated for the treatment of mesothelioma and multiple forms cancers, show great promise clinical development solid cancers. Antibodies have been shown to act via immunotoxin-based inhibition tumor growth induction antibody-dependent cell-mediated cytotoxicity (ADCC). However, complement-dependent (CDC), considered an important additional mechanism therapeutic tumors, is inactive such antibodies. Here, we used phage display antibody...
Glypican-3 (GPC3) is overexpressed in hepatocellular carcinoma (HCC). We constructed a recombinant immunotoxin, HN3-mPE24, which contains truncated form of Pseudomonas exotoxin A. The toxin portion lacks most domain II and has seven point mutations III to remove the B-cell epitopes thought be responsible for causing off-target side effects immunogenicity. also fused bivalent HN3 mPE24. tested these two molecules GPC3 binding cytotoxicity HCC cell models. KD values HN3-mPE24 HN3-HN3-mPE24...
Abstract Glypican-3 (GPC3) is a cell surface heparan sulfate proteoglycan that being evaluated as an emerging therapeutic target in hepatocellular carcinoma (HCC). GPC3 has been shown to interact with several extracellular signaling molecules, including Wnt, HGF, and Hedgehog. Here, we reported transmembrane protein (FAT1) new interacting protein. The binding region on FAT1 was initially mapped the C-terminal (Q14517, residues 3662-4181), which covered putative receptor tyrosine phosphatase...
Background Tumor microenvironments present significant barriers to penetration by antibodies and immunoconjugates. microenvironments, however, are difficult study in vitro. Cells cultured as monolayers exhibit less resistance therapy than those grown vivo an alternative research model more representative of the tumor is desirable. SS1P immunotoxin composed Fv portion a mesothelin-specific antibody fused bacterial toxin that presently undergoing clinical trials mesothelioma....
Choline participates in three major metabolic pathways: oxidation, phosphorylation, and acetylation. Through choline is converted to betaine contributes methyl metabolism epigenetic regulation. phospholipid metabolism, serves as the precursor of phosphocholine, phosphatidylcholine, glycerophosphocholine, other essential compounds, thereby modulating lipid transport. acetylation, transformed into acetylcholine cholinergic neurons, playing a vital role neurotransmission. Moreover, gut...
Cytopathology, crucial in disease diagnosis, commonly uses microscopic slides to scrutinize cellular abnormalities. However, processing high volumes of samples often results numerous negative diagnoses, consuming significant time and resources healthcare. To address this challenge, a surface acoustic wave-enhanced multi-view acoustofluidic rotation cytometry (MARC) technique is developed for pre-cytopathological screening. MARC enhances morphology analysis through comprehensive multi-angle...
A single G-quadruplex forming sequence from the human telomere can adopt six distinct topologies that are inter-convertible under physiological conditions. This presents challenges to design ligands show selectivity and specificity towards a particular conformation. Additional complexity is introduced in differentiating multimeric G-quadruplexes over monomeric species, which would be able form single-stranded 3' ends of telomeres. few have been reported bind dimeric quadruplexes, but their...
Abstract SARS‐CoV‐2 is the etiological agent of COVID‐19 pandemic. Antibody‐based therapeutics targeting spike protein, specifically S1 subunit or receptor binding domain (RBD) SARS‐CoV‐2, have gained attention due to their clinical efficacy in treating patients diagnosed with COVID‐19. An alternative conventional antibody use shark new antigen variable (V NAR ) antibodies. V s are small (<15 kDa) and can reach deep into pockets grooves target antigen. Here, we isolated 53 that bind S2 by...