A5100368178- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Cancer Immunotherapy and Biomarkers
- Hippo pathway signaling and YAP/TAZ
- Thyroid Cancer Diagnosis and Treatment
- Integrated Circuits and Semiconductor Failure Analysis
- Neonatal and fetal brain pathology
- Gut microbiota and health
- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Advancements in Semiconductor Devices and Circuit Design
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic and Hepatic Oncology Research
- Salivary Gland Disorders and Functions
- Cancer-related Molecular Pathways
- Connective tissue disorders research
Shanghai Advanced Research Institute
2023
Chinese Academy of Sciences
2023
Allen Institute
2022-2023
University of Science and Technology of China
2023
Hefei National Center for Physical Sciences at Nanoscale
2023
Nanjing Medical University
2014-2022
Xuzhou Central Hospital
2022
Koch Institute for Integrative Cancer Research At MIT
2022
Jiangsu Province Hospital
2022
National Health and Family Planning Commission
2021
The effectiveness of chimaeric antigen receptor (CAR) T cell therapies for solid tumours is hindered by difficulties in the selection an effective target antigen, owing to heterogeneous expression tumour antigens and healthy tissues. Here we show that cells with a CAR specific fluorescein isothiocyanate (FITC) can be directed against via intratumoural administration FITC-conjugated lipid-poly(ethylene)-glycol amphiphile inserts itself into membranes. In syngeneic human xenografts mice,...
Wnt signaling is one of the key regulators hepatocellular carcinoma (HCC) tumor progression. In addition to classical receptor frizzled (FZD), various coreceptors including heparan sulfate proteoglycans (HSPGs) are involved in activation. Glypican‐3 (GPC3) an HSPG that overexpressed HCC and functions as a coreceptor modulates cell proliferation. These features make GPC3 attractive target for liver cancer therapy. However, precise interaction how GPC3, Wnt, FZD cooperate with each other...
Background Glypican-3 (GPC3), a cell surface glycoprotein that is pathologically highly expressed in hepatocellular carcinoma (HCC), an attractive target for immunotherapies, including chimeric antigen receptor (CAR) T cells. The serum GPC3 frequently elevated HCC patients due to the shedding effect of GPC3. shed (sGPC3) reported block function cell-surface as negative regulator. Therefore, it would be worth investigating potential influence anti-GPC3 CAR-T therapy HCC. Methods In this...
The topographical features and material composition of scaffolds have a powerful influence on cell behaviors such as proliferation differentiation. Here, consisting aligned fibers with incorporated bioactive collagen I were tested for their ability to enhance osteogenesis in vitro. Rat adipose-derived mesenchymal stem cells (ADSCs) seeded the morphology, proliferation, osteogenic differentiation examined. Aligned showed best properties. Also, adhesion-related genes higher expression I. Our...
Significant differences in salivary microbiota communities between polycystic ovary syndrome (PCOS) patients and healthy controls have been reported, interestingly, some exhibit diurnal oscillation people. However, whether the of is present PCOS unknown. In this study, we describe saliva microbiome group control at different time points over 24 h. 16S rRNA gene amplicon sequencing was performed on fecal samples from 10 controls, were collected 6-h intervals h (Zeitgeber (ZT)0, ZT6, ZT12,...
Aim: This study aimed to investigate the distinct expression pattern of circular RNAs (circRNAs) in stem cells from apical papilla (SCAPs) during osteogenesis. Materials & methods: Isolated SCAPs were cultured growth medium or osteogenic medium, respectively. Total RNA was extracted and submitted RNA-sequencing. Expression profiles circRNAs constructed circRNA–miRNA–mRNA networks determined. Results: A total 333 unregulated 317 downregulated differentiation detected. Bioinformatics analysis...
Long non-coding RNA (lncRNA) growth arrest specific 5 (GAS5) and microRNA (miR)-146a both have inhibitory effects on LPS-induced inflammation, suggesting the crosstalk between them. In this study, expression of GAS5 miR-146a in patients with sepsis-induced acute lung injury (sepsis-ALI), sepsis without obvious complications (sepsis) healthy controls were studied by RT-qPCR. The role methylation human bronchial epithelial cells (HBEpCs) RT-qPCR methylation-specific PCR (MSP), respectively....
A nonwoven membrane for guided bone regeneration, constituting of poly(butylene carbonate), with a backbone that is similar to poly(ϵ-caprolactone), was prepared by electrospinning. The as-fabricated carbonate) membranes were be used as regeneration efficacies equal or better than poly(ϵ-caprolactone) membranes. contact angles electrospun (fPBC) (101.90 ± 4.19°) lower those (fPCL) (117.79 3.38°) ( p < 0.01). To examine the biocompatibility, we investigated cell morphology, proliferation,...
Abstract Ligand-induced receptor dimerization or oligomerization is a widespread mechanism for ensuring communication specificity, safeguarding activation, and facilitating amplification of signal transduction across the cellular membrane. However, cell-surface antigen-induced multimerization (dubbed AIM herein) has not yet been consciously leveraged in chimeric antigen (CAR) engineering enriching T cell-based therapies. We co-developed ciltacabtagene autoleucel (cilta-cel), whose CAR...
Aggressive medullary thyroid carcinomas (MTC) have a high mortality rate and the treatment for patients diagnosed with advanced MTC is comparatively ineffective. We hence aimed to test effects of miR-376c-3p on explore relevant mechanism.Cell Counting Kit-8 (CCK-8) soft agar colony formation assay were applied evaluate proliferation transfected MZ-CRC-1 cells. Wound healing transwell employed cell migration invasion, respectively. Luciferase was performed validate downstream target in...
Abstract Chimeric Antigen Receptor (CAR) T-cell therapy can be effective in treating human cancer but loss of the antigen recognized by CAR poses a major obstacle. Here, we report an approach for vaccine boosting T-cells, which triggers engagement endogenous immune system to circumvent antigen-negative tumor escape. Vaccine-boosted T-cells promoted dendritic cell (DC) recruitment tumors, increased uptake DCs, and elicited priming anti-tumor (antigen spreading). This process was accompanied...
The treatment of hepatocellular carcinoma (HCC) involves multidisciplinary clinical divisions and patients may be admitted to different disciplinary nature. Few studies have assessed the potential effect hospital admissions into on patient options survival. This study aimed analyze this effect.We analyzed data HCC between 2002 2011 in a large northwest China compared outcomes following initial admission two major divisions: internal medicine surgical. Barcelona Clinic Liver Cancer criteria...
Objective Intraventricular hemorrhage (IVH) is a serious neurological complication in premature infants. This study aimed to investigate the white matter impairments and neurodevelopmental outcomes of severe IVH extremely preterm infants with gestation age less than 28 weeks. Methods We retrospectively evaluated between 2017 2020. Neurodevelopmental were Bayley Scales Infant Toddler Development-III at 2 years corrected age. Diffusional kurtosis imaging (DKI) was employed evaluate...
<div>Abstract<p>Target-mediated toxicity is a major limitation in the development of chimeric antigen T-cell receptors (CAR) for adoptive cell therapy solid tumors. In this study, we developed strategy to adjust affinities scFv component CAR discriminate tumors overexpressing target from normal tissues that express it at physiologic levels. A CAR-expressing panel was generated with varying over three orders magnitude. High-affinity cells recognized expressed any level, including...
<div>Abstract<p>Target-mediated toxicity is a major limitation in the development of chimeric antigen T-cell receptors (CAR) for adoptive cell therapy solid tumors. In this study, we developed strategy to adjust affinities scFv component CAR discriminate tumors overexpressing target from normal tissues that express it at physiologic levels. A CAR-expressing panel was generated with varying over three orders magnitude. High-affinity cells recognized expressed any level, including...
The membrane trafficking of cation-independent mannose 6-phosphate receptor (CI-M6PR) between the trans-Golgi network (TGN) and endosomal compartments is not only critical for maintaining lysosomal function but also a well-known event understanding molecular cellular mechanisms in retrograde endosome-to-TGN trafficking. Although it has been well established literature that C-terminus bovine CI-M6PR determines its trafficking, remains unclear whether luminal domain protein plays role on these...
Li, Yin-Ping1,5; Paczesny, Sophie2; Latger-Canard, Véronique3; Marchal, Luc4; Na1; Stoltz, Jean-François1; Yang, Jing-Ping5; Eljaafari, Assia1 Author Information