A5101447041- CRISPR and Genetic Engineering
- HIV Research and Treatment
- interferon and immune responses
- Mosquito-borne diseases and control
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Innovation and Socioeconomic Development
- Viral Infections and Vectors
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Immune cells in cancer
- Viral-associated cancers and disorders
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Renal and related cancers
- Vector-Borne Animal Diseases
- Renal cell carcinoma treatment
Wuhan University
2015-2025
State Key Laboratory of Virology
2013-2024
Wuhan Institute of Virology
2013-2024
Zhongnan Hospital of Wuhan University
2023
Nanchang University
2022
First Affiliated Hospital of Jiangxi Medical College
2022
Jiangxi Provincial People's Hospital
2022
The Ohio State University
2017-2019
Hubei University
2013
Abstract Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes the genome of living cells. CXCR4 is a co-receptor for human immunodeficiency virus type 1 (HIV-1) infection been considered as important therapeutic target AIDS. mediates viral entry into CD4 + cells by binding envelope protein, gp120. Here, we show that gene efficiently disrupted CRISPR/Cas9-mediated editing, leading HIV-1 resistance primary T We also Cas9-mediated ablation...
The main approach to treat HIV-1 infection is combination antiretroviral therapy (cART). Although cART effective in reducing viral load and controlling disease progression, it has many side effects, expensive for infected patients who must remain on lifetime treatment. gene drawn much attention as studies of genome editing tools have progressed. For example, zinc finger nucleases (ZFN), transcription activator like effector (TALEN) clustered regularly interspaced short palindromic repeats...
Hepatitis B virus (HBV) remains a global health threat as chronic HBV infection may lead to liver cirrhosis or cancer. Current antiviral therapies with nucleoside analogues can inhibit the replication of HBV, but do not disrupt already existing covalently closed circular DNA. The newly developed CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated 9) system is powerful tool target cellular genome DNA for gene editing. In order investigate possibility...
Sterile alpha motif and HD-domain-containing protein 1 (SAMHD1) blocks replication of retroviruses certain DNA viruses by reducing the intracellular dNTP pool. SAMHD1 has been suggested to down-regulate IFN inflammatory responses viral infections, although functions mechanisms in modulating innate immunity remain unclear. Here, we show that suppresses immune infections stimuli inhibiting nuclear factor-κB (NF-κB) activation type I interferon (IFN-I) induction. Compared with control cells,...
The chemokine receptor CCR5, which belongs to the superfamily of G protein-coupled receptors, is major co-receptor for HIV-1 entry. Individuals with a homozygous CCR5Δ32 mutation have long lasting and increased resistance infection. Therefore, CCR5 represents an optimal target HIV-1/AIDS gene therapy. CRISPR/Cas9 system has been developed as one most efficacious editing tools in mammalian cells small-sized version from Staphylococcus aureus (SaCas9) advantage easier delivery compared...
The CRISPR/Cas9 system has been widely used for genome editing in mammalian cells. CXCR4 is a co-receptor human immunodeficiency virus type 1 (HIV-1) entry, and loss of function can protect cells from (X4)-tropic HIV-1 infection, making an important target gene therapy. However, the large size CRISPR/SpCas9 presents obstacle to its efficient delivery into primary CD4+ T Recently, small Staphylococcus aureus Cas9 (SaCas9) developed as tool address this question. Therefore, it provides...
The C-X-C chemokine receptor type 4 (CXCR4) is one of the major co-receptors for human immunodeficiency virus 1 (HIV-1) entry and considered an important therapeutic target. However, its function in maintaining development hematopoietic stem cells (HSC) makes it difficult to be used HIV-1 gene therapy with HSC transplantation. A previous report showed that natural CXCR4 P191A mutant inhibits infection without any defect differentiation, which could provide a basis new approaches therapy. In...
The chemokine receptor CCR5 is one of the co-receptor HIV-1 infection. People with homozygous CCR5Δ32 deletion resist infection, which makes an important target for gene therapy. Although CRISPR/Cas9 has ever been used study, newly developed CRISPR/AsCpf1 never utilized in disruption. CRISPR/Cpf1 system shows many advantages over CRISPR/Cas9, such as lower off-target, small size nuclease, easy sgRNA design multiplex editing, etc. Therefore, mediated editing will confer a more specific and...
Inactivation or mutation of the VHL gene causes various tumors, including clear cell renal carcinoma (ccRCC). In present study, we identified ZBRK1 as a novel interacting protein by yeast two-hybrid screening, and found single ZBRK1-binding site located in promoter region. Ectopic expression increases transcriptional activity VHL, whereas depletion endogenous shRNA leads to reduction expression. We also demonstrate that inhibition VEGF transcription overexpression is dependent on VHL/HIF...
The translationally controlled tumour protein (TCTP) plays an important role in maintaining cell proliferation and its high expression is associated with many tumours. suppressor von Hippel-Lindau (VHL) has been shown to function as E3 ubiquitin ligase. Although great progress made, biological roles of these factors relevant molecular mechanisms remain largely unknown.In this study, we have that TCTP specifically binds VHL through β domain competes hypoxia-inducible factor-1α (HIF1α)....
Loss and/or inactivation of the VHL (von Hippel-Lindau) tumour suppressor causes various tumours. Using a yeast two-hybrid system, we have identified AR (androgen receptor) co-activator UXT (ubiquitously expressed transcript), as VHL-interacting protein. GST pull-down and co-immunoprecipitation assays show that interacts with VHL. In addition, recruits to nucleus. associates DBD (DNA-binding domain) hinge domains induces ubiquitination. Moreover, interaction activates transactivation upon...
A20-binding inhibitor of NF-κB activation (ABIN1), an important immune regulator, was previously shown to be involved in HIV-1 replication. However, the reported studies done with overexpressed ABIN1 provided controversial results.Here we identified as a suppressor transcription since transient knockdown led increased replication both transformed Jurkat T cell line and primary human CD4+ lymphocytes. Depletion specifically enhanced from integrated genome during viral life cycle, but not...