A5065214173- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Respiratory viral infections research
- Immunodeficiency and Autoimmune Disorders
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Outbreaks Research
- Vaccine Coverage and Hesitancy
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Pediatric health and respiratory diseases
- Infant Health and Development
- Long-Term Effects of COVID-19
- Nursing education and management
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Transgenic Plants and Applications
- Hibiscus Plant Research Studies
- Hematological disorders and diagnostics
University of Iowa
2020-2025
Wuhan University
2009-2022
Wuhan Institute of Virology
2021-2022
Hubei Provincial Center for Disease Control and Prevention
2017
Swiss Red Cross
1968
HES-SO Genève
1959
Columbia University
1949
The 5' cap structures of eukaryotic mRNAs are important for RNA stability and protein translation. Many viruses that replicate in the cytoplasm eukaryotes have evolved 2'-O-methyltransferases (2'-O-MTase) to autonomously modify their carry a cap-1 structure (m7GpppNm) at end, thereby facilitating viral replication escaping innate immune recognition host cells. Previous studies showed 2'-O-MTase activity severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural 16 (nsp16) needs...
The ongoing coronavirus (CoV) disease 2019 (COVID-19) pandemic caused by infection with severe acute respiratory syndrome CoV 2 (SARS-CoV-2) is associated substantial morbidity and mortality. Understanding the immunological pathological processes of diseases crucial for rational design effective vaccines therapies COVID-19. Previous studies showed that 2'-O-methylation viral RNA cap structure required to prevent recognition RNAs intracellular innate sensors. Here, we demonstrate guanine...
To gain insights into the pathogenesis of influenza A virus (IAV) infections, this study focused on characterizing inflammatory network and identifying key proteins by combining high-throughput data computational techniques. We constructed cell-specific normal networks for H5N1 H1N1 infections through integrating data. demonstrated that better discrimination between entropy than other topological metrics. Moreover, we identified different dynamical interactions among TLR2, IL-1β, IL10 NFκB...
Significance Both genetic and environmental factors, especially viruses, may contribute to the development of multiple sclerosis. In this study, we find that oligodendrocytes (OLs) survive infection with a neurotropic coronavirus, mouse hepatitis virus, for at least 150 d. These surviving OLs are located adjacent demyelinating regions express genes chronic inflammatory state. Notably, extent cell infiltration subsequent ability regain normal OL morphology varied depending upon anatomic...
The COVID-19 pandemic exposed limitations of conventional antibodies as therapeutics, including high cost, limited potency, ineffectiveness against new viral variants, and primary reliance on injection-only delivery. Nanobodies are single-domain with therapeutic potentials. We discovered three anti-SARS-CoV-2 nanobodies, named Nanosota-2, -3, -4, from an immunized alpaca. Nanosota-2 is super potent prototypic SARS-CoV-2, Nanosota-3 highly the omicron variant, Nanosota-4 effective both...
A major challenge in antiviral antibody therapy is keeping up with the rapid evolution of viruses. Our research shows that nanobodies—single-domain antibodies derived from camelids—can be rapidly re-engineered to combat new viral strains through structure-guided vitro evolution. Specifically, for mutations occurring at nanobody-binding sites, we introduce randomized amino acid sequences into nanobody residues near these mutations. We then select variants effectively bind mutated target a...
Coronaviruses (CoVs) can infect a variety of hosts, including humans, livestock and companion animals, pose serious threat to human health the economy. The current COVID-19 pandemic, which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has killed millions people. Unfortunately, effective treatments for CoVs infection are still lacking, suggesting importance vaccines. Our previous work showed that CoV nonstuctural protein 14 (nsp14) functions as...
Grasping the roles of epitopes in viral glycoproteins is essential for unraveling structure and function these proteins. Up to now, all identified have been found either neutralize, no effect on, or enhance entry into cells. Here, we used nanobodies (single-domain antibodies) as probes investigate a unique epitope on SARS-CoV-2 spike protein, located outside protein's receptor-binding domain. Nanobody binding this enhances cell prototypic SARS-CoV-2, while neutralizing Omicron variant....
ABSTRACT Post-acute sequelae of COVID-19 involves several organs, but its basis remains poorly understood. Some infected cells in mice survive the acute infection and persist for extended periods respiratory tract not other tissues. Here, we describe two experimental models severe syndrome coronavirus-2 (SARS-CoV-2) to assess effect viral virulence on previously cells. Both approaches use lineage tracking In with a highly pathogenic mouse-adapted SARS-CoV-2, alveolar type 2 (AT2) 1 (AT1)...
The investigation of the dynamics and regulation virus-triggered innate immune signaling pathways at a system level will enable comprehensive analysis complex interactions that maintain delicate balance between resistance to infection viral disease. In this study, we developed delayed mathematical model describe virus-induced interferon (IFN) process by considering several key players in response. Using dynamic numerical simulation, evaluated following predictions regarding antiviral...
IN VITRO and in vivo properties of the attenuated Cendehill strain rubella virus have been described previously. This has by serial propagation primary rabbit kidney cells. It is characterized production an early cytopathic effect cultures, formation distinct plaques RK<sub>13</sub>cells, loss intranasal infectivity for monkey, immunogenicity after subcutaneous inoculation.<sup>1,2</sup>Preliminary clinical trials with at 21st 51st passage levels reported previously.<sup>3-6</sup> Although...
ABSTRACT Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) has resulted in substantial morbidity and mortality. The basis of humans is difficult to determine without the use experimental animal models. Mice are resistant infection with ancestral strains SARS-CoV-2, although many variants that arose later pandemic were able directly infect mice. In almost all cases, viruses naturally infected mice or engineered enable mouse required...
To the Editor: Long-term sequelae of SARS-CoV-2 infection, referred to as postacute COVID-19 (PASC), involve many organs, including cardiovascular, pulmonary, gastrointestinal, and neurological systems.The basis PASC is not well understood, but one possible explanation that persistence infectious virus or viral RNA protein contributes PASC.This difficult assess in patients because human tissue can only usually be assessed for products at autopsy by biopsy.Furthermore, it known if some...