A5035916673- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Reconstructive Surgery and Microvascular Techniques
- Metabolomics and Mass Spectrometry Studies
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Orthopedic Surgery and Rehabilitation
- Protist diversity and phylogeny
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Advanced Proteomics Techniques and Applications
- Bone fractures and treatments
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Chinese history and philosophy
- Reconstructive Facial Surgery Techniques
- Heavy metals in environment
- Microbial Fuel Cells and Bioremediation
- China's Socioeconomic Reforms and Governance
- Anaerobic Digestion and Biogas Production
- Single-cell and spatial transcriptomics
- Enzyme Structure and Function
Ministry of Education of the People's Republic of China
2016-2025
Tianjin University
2016-2025
Anhui Provincial Hospital
2023-2025
Earth Island Institute
2011-2024
Columbia University
2011-2024
Zhejiang University
2014-2024
XinHua Hospital
2015-2024
Quzhou City People's Hospital
2013-2024
Wenzhou Medical University
2016-2024
Shanghai Jiao Tong University
2024
Dissimilatory sulfate-reducing prokaryotes (SRB) are a very diverse group of anaerobic bacteria that omnipresent in nature and play an imperative role the global cycling carbon sulfur. In anoxic marine sediments sulfate reduction accounts for up to 50% entire organic mineralization coastal shelf ecosystems where diffuses several meters deep into sediment. As consequence, SRB would be expected sulfate-containing upper sediment layers, whereas methanogenic archaea succeed deeper...
Abstract The modest correlation between mRNA expression and protein abundance in large-scale data sets is explained part by experimental challenges, such as technological limitations, fundamental biological factors the transcription translation processes. Among various affecting mRNA–protein correlation, roles of related to are poorly understood. In this study, using collected from Desulfovibrio vulgaris DNA microarray liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS)...
Cyanobacteria and algae are becoming increasingly attractive cell factories for producing renewable biofuels chemicals due to their ability capture solar energy CO2 relatively simple genetic background manipulation. Increasing research efforts from the synthetic biology approach have been made in recent years modify cyanobacteria various biotechnological applications. In article, we critically review progresses developing tools characterizing or manipulating algae, applications of...
Recent progress in metabolic engineering has led to autotrophic production of ethanol various cyanobacterial hosts. However, cyanobacteria are known be sensitive ethanol, which restricts further efforts increase levels these renewable host systems. To understand the mechanisms tolerance so that more robust hosts can possible, this study, responses model Synechocystis sp. PCC 6803 were determined using a quantitative proteomics approach with iTRAQ LC-MS/MS technologies. The resulting...
Docosahexaenoic acid (DHA, C22:6) and odd-chain fatty acids (OCFAs, C15:0 C17:0) have attracted great interest, since they been widely used in food therapeutic industries, as well chemical industry, such biodiesel production improvement. The oil-producing heterotrophic microalgae Schizochytrium sp. 31 is one of main DHA-producing strains. Recently, it was found that can also synthesize OCFAs; however, contents titers DHA OCFAs are still low, which limit its practical application. In this...
Synthetic microbial consortia represent a new frontier for synthetic biology given that they can solve more complex problems than monocultures. However, most attempts to co-cultivate these artificial communities fail because of the winner-takes-all in nutrients competition. In soil, multiple species coexist with spatial organization. Inspired by nature, here we show an engineered segregation method assemble stable both flexibility and precision. We create swarmbot (MSBC) encapsulating...
The discovery of the gut–brain axis has proven that brain functions can be affected by gut microbiota's metabolites, so there are significant opportunities to explore new tools regulate microbiota and thus work on functions. Meanwhile, engineered bacteria as oral live biotherapeutic agents host's healthy homeostasis have attracted much attention in microbial therapy. However, whether this strategy is able remotely function vivo not been investigated. Here, we three blue-light-responsive...
Nanozymes have been widely studied as substitutes for natural enzymes. However, the delicacy of their structures and unclear catalytic sites make it difficult to maintain structural robustness durability. By mimicking active enzymes combining them with distinct channels metal organic frameworks (MOFs), an copper mimetic oxidase enzyme (Cu-MOF) was designed synthesized good structure clear improvement in activity. The Cu-MOFs showed excellent oxidase-like activity a low Km 1.09 mM exogenous...
The pollution caused by heavy metals (HMs) represents a global concern due to their serious environmental threat. Photosynthetic cyanobacteria have natural niche and the ability remediate HMs such as cadmium. However, practical application is hindered low tolerance issues related recycling. In response these challenges, this study focuses on development evaluation of engineered cyanobacteria-based living materials for bioremediation. Genes encoding phytochelatins (
Gene-inactivation studies point to the involvement of OxyB in catalyzing first oxidative phenol coupling reaction during glycopeptide antibiotic biosynthesis. The oxyB gene has been cloned and sequenced from vancomycin producer Amycolatopsis orientalis, hemoprotein produced Escherichia coli, crystallized, its structure determined 1.7-A resolution. gave UV-visible spectra characteristic a P450-like low spin ferric state. After reduction ferrous state by dithionite or spinach ferredoxin...
Abstract Background Many Firmicutes bacteria, including solvent-producing clostridia such as Clostridium acetobutylicum , are able to utilize xylose, an abundant carbon source in nature. Nevertheless, homology searches failed recognize all the genes for complete xylose and xyloside utilization pathway most of them. Moreover, regulatory mechanisms catabolism many except Bacillus spp. still remained unclear. Results A comparative genomic approach was used reconstruct analyze its 24 genomes...
Highly expressed genes in bacteria often have a stronger codon bias than at lower levels, due to translational selection. In this study, comparative analysis of predicted highly (PHX) the Streptomyces coelicolor and avermitilis genomes was performed using adaptation index (CAI) as numerical estimator gene expression level. Although it has been suggested that there is little heterogeneity usage G+C-rich bacteria, considerable found among these two genomes. Using ribosomal protein references,...