A5100677413- Occupational and environmental lung diseases
- Cancer Research and Treatments
- CAR-T cell therapy research
- Toxin Mechanisms and Immunotoxins
- Cancer Immunotherapy and Biomarkers
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Bacillus and Francisella bacterial research
- Pleural and Pulmonary Diseases
- Microbial infections and disease research
- Neuroblastoma Research and Treatments
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Nosocomial Infections in ICU
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunotoxicology and immune responses
- Microbial Inactivation Methods
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Phytochemicals and Antioxidant Activities
- Pancreatic and Hepatic Oncology Research
- Pregnancy and preeclampsia studies
Guangdong Pharmaceutical University
2015-2025
National Cancer Institute
2011-2024
Center for Cancer Research
2011-2024
National Institutes of Health
2011-2024
Qingdao University
2017-2024
Affiliated Hospital of Qingdao University
2017-2024
Jiangnan University
2024
Wuhan University of Technology
2024
Qingdao Eighth People's Hospital
2023
Henan Provincial People's Hospital
2019-2023
Abstract Purpose: To determine whether mesothelin, a cell surface protein highly expressed in mesothelioma and ovarian cancer, is shed into serum if so to accurately measure it. Experimental Design: We developed sandwich ELISA using antibodies reacting with two different epitopes on human mesothelin. quantitate mesothelin levels, standard curve was generated mesothelin-Fc fusion protein. Sera from 24 healthy volunteers, 95 random hospital patients, 56 patients mesothelioma, 21 cancer were...
Ovarian cancer and malignant mesothelioma frequently express both mesothelin CA125 (also known as MUC16) at high levels on the cell surface. The interaction between may facilitate implantation peritoneal spread of tumors by adhesion, whereas detailed nature this is still unknown. Here, we used truncated mutagenesis alanine replacement techniques to identify a binding site for CA125. We examined molecular Western blot overlay assays further quantitatively analyzed enzyme-linked immunosorbent...
BACKGROUND The primary objective of this study was to determine the safety and maximum tolerated dose (MTD) antimesothelin immunotoxin SS1(dsFv)PE38 (SS1P) (a recombinant consisting a murine variable antibody fragment [Fv] linked PE38, truncated portion Pseudomonas exotoxin A) in combination with pemetrexed cisplatin chemotherapy‐naive patients advanced malignant pleural mesothelioma (MPM). Secondary objectives included tumor response, SS1P pharmacokinetics, serum biomarkers response....
Significance Survival from malignant mesothelioma, particularly pleural is very poor. For patients with some cancers, overall survival following platinum chemotherapy better for germline mutations in DNA repair and related genes than without such mutations. We evaluated this relationship mesothelioma. Following chemotherapy, was significantly longer loss-of-function BAP1 compared no The effect of genotype highly significant but not peritoneal remained after adjusting gender age at diagnosis....
Mesothelin is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface. differentiation antigen that highly expressed mesothelioma, ovarian cancer, and pancreatic cancer. The existence of spontaneous humoral immune response to mesothelin in humans has not been fully studied. Here we addressed issue whether elicits patients with mesothelioma cancer.Using an ELISA, analyzed immunoglobulin G antibodies specific for sera from epithelial Tumor specimens were examined by...
SS1P is a recombinant immunotoxin (RIT) engineered for the targeted elimination of malignant cells that express tumor-associated antigen mesothelin. It composed an antimesothelin antibody variable fragment (Fv) linked to cytotoxic Pseudomonas exotoxin A (PE) includes domains II and III native PE. The clinical use limited by its propensity induce neutralizing antibodies cause dose-limiting capillary leak syndrome (CLS) in patients. In this article, we describe reengineered with improved...
BRCA1-associated protein-1 (BAP1), a nuclear deubiquitinase thought to be involved in DNA double-strand break repair, is frequently mutated mesothelioma. Because poly(adenosine diphosphate-ribose) polymerase inhibitors (PARPIs) induce synthetic lethality BRCA1/2 mutant cancers, we evaluated whether BAP1 inactivating mutations confer sensitivity PARPIs mesothelioma and if combination therapy with temozolomide (TMZ) would beneficial.A total of 10 patient-derived cell lines were generated...
Preeclampsia (PE) is a pregnancy-specific syndrome that substantially leads to maternal and fetal mortality. Multiple factors contribute the disease, but exact pathogenesis still remains elusive. Here we explored roles of lncRNA MALAT1 miR-206 in PE. qRT-PCR was applied measure mRNA levels placenta PE patients. Scratch wound healing assay transwell invasion were conducted test effects on migration trophoblast cells. In addition, validated MALAT1/miR-206 miR-206/IGF-1 interactions with dual...
Abstract Mesothelin targeting CAR T cells have limited activity in patients. In this study, we sought to determine if efficacy of anti-mesothelin is dependent on the mesothelin epitopes that are recognized by them. To do so, developed hYP218 (against membrane-proximal epitope) and SS1 membrane-distal cells. Their was assessed vitro using mesothelin-positive tumor cell lines vivo NSG mice with mesothelin-expressing ovarian cancer (OVCAR-8), pancreatic (KLM-1), mesothelioma patient-derived...
Abstract The assembly of prereplicative complex (pre-RC) during G1 phase must be tightly controlled to sustain cell proliferation and maintain genomic stability. Mechanisms prevent pre-RC formation in G2/M S phases are well appreciated, whereas how cells ensure efficient is less clear. Here we report that cyclin K regulates formation. We find expression positively correlates with proliferation, knockdown or its cognate kinase CDK12 prevents the phase. Mechanistically uncover promotes by...
Malignant mesothelioma is an aggressive cancer with limited treatment options and poor prognosis. A better understanding of genomics transcriptomics could advance therapies. Here, we present a cohort 122 patients along their germline tumor whole-exome RNA sequencing data as well phenotypic drug response information. We identify 48-gene prognostic signature that highly predictive patient survival, including CCNB1, the expression which survival on its own. In addition, analyze to study immune...
Studies on the dynamic changes occurring in tumor microenvironment (TME) following CAR-T cell therapy have been confounded by host lymphodepletion, multiple dosing and immunodeficient models. Here, a nanobody-based, mouse mesothelin-targeting (A101) was developed, achieving effective primary suppression, metastasis reduction, improved survival after single dose immunocompetent, syngeneic models without lymphodepletion. Temporal profiling using RNA sequencing revealed initial downregulation...
Background/Objectives: BuZhong Yiqi Formula (BZYQF) has significant ameliorative effects on type 2 diabetes mellitus (T2DM). However, its efficacy in alleviating the hyposalivation caused by T2DM needs to be confirmed, and mechanism is unclear. Methods: Network pharmacology molecular docking were combined analyze which BZYQF alleviates T2DM-caused hyposalivation. A rat model was induced evaluate of BZYQF. The total saliva before after acid stimulation collected determine salivary flow rate...
Inducible nitric oxide (NO) synthase (iNOS) is comprised of an oxygenase domain containing heme, tetrahydrobiopterin, the substrate binding site, and a reductase FAD, FMN, calmodulin, NADPH site. Enzyme activity requires dimeric interaction between two domains with attached as monomeric extensions. To understand how dimerization activates iNOS, we synthesized iNOS heterodimer one full-length subunit histidine-tagged that was missing its domain. The purified using nickel-Sepharose 2′,5′-ADP...
Abstract Purpose: To determine the antitumor activity of anti–mesothelin immunotoxin SS1P in combination with gemcitabine against mesothelin-expressing tumor xenografts. Experimental Design: The vitro cell line A431/K5 was evaluated using cytotoxicity and apoptosis assays. this nude mice bearing Tumor-bearing were treated different doses schedules alone, alone (0.2 mg/kg i.v. every other day × three doses), or both agents together, volumes measured over time. Results: In studies failed to...
The China-Anhui Birth Cohort Study (C-ABCS) was set up to examine the delayed, cumulative and interactive effects of maternal environmental exposures on birth outcomes children's development. C-ABCS recruited pregnant women from six major cities Anhui province, China, between November 2008 October 2010. A range data (including demographic, obstetric, occupational, nutritional psychosocial factors) were collected by both interviews laboratory tests. In each trimester, women's blood samples...
The cross-talk between tumor cells, myeloid and T cells can play a critical role in pathogenesis response to immunotherapies. Although the etiology of mesothelioma is well understood, impact on surrounding immune microenvironment less studied. In this study, effect circulating infiltrating granulocytes investigated.
// Ronit Mazor 1 , Masanori Onda Dong Park 1,3 Selamawit Addissie Laiman Xiang 1,* Jingli Zhang 2 Raffit Hassan and Ira Pastan Laboratory of Molecular Biology, Center for Cancer Research, National Institute, Institutes Health, Bethesda, Maryland, USA Thoracic GI Oncology Branch, 3 New Business Development Department, Medytox Inc., Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea * Retired Correspondence to: Pastan, email: Keywords : epitope, immunogenicity, rational design, mesothelioma,...
Mesothelin-targeted immunotoxin LMB-100 with anti–PD-1 antibody enhanced antitumor activity in patients mesothelioma and mouse tumor models.