A5101770354- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Extracellular vesicles in disease
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- biodegradable polymer synthesis and properties
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
- Cancer, Hypoxia, and Metabolism
- Genetic Syndromes and Imprinting
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Paraquat toxicity studies and treatments
- Clusterin in disease pathology
- Urinary Tract Infections Management
- Enzyme Production and Characterization
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- RNA Research and Splicing
Kunming Medical University
2011-2024
First Affiliated Hospital of Kunming Medical University
2011-2024
Zhengzhou University
2019-2021
Henan Provincial People's Hospital
2017-2021
ShanghaiTech University
2019-2021
Wenzhou Medical University
2021
Dongyang People's Hospital
2021
Shanghai Advanced Research Institute
2019-2020
University of Chinese Academy of Sciences
2019-2020
Chinese Academy of Sciences
2019-2020
Nuclear enriched abundant transcript 1 (NEAT1) has been demonstrated to act as a tumor inhibitor in many cancers. However, the role of NEAT1 development ovarian cancer (OC) remains far from being elaborated. Hence, aim this study is investigate expression and function OC.The level was determined by quantitative real-time polymerase chain reaction OC cell lines. MTT assay, caspase-3 activity flow cytometry analysis were conducted effects NEAT1, miR-34a-5p, or B-cell lymphoma-2 (BCL2) on...
Preeclampsia (PE) is a pregnancy-specific syndrome that substantially leads to maternal and fetal mortality. Multiple factors contribute the disease, but exact pathogenesis still remains elusive. Here we explored roles of lncRNA MALAT1 miR-206 in PE. qRT-PCR was applied measure mRNA levels placenta PE patients. Scratch wound healing assay transwell invasion were conducted test effects on migration trophoblast cells. In addition, validated MALAT1/miR-206 miR-206/IGF-1 interactions with dual...
Studies have shown that lncRNA DANCR is down-regulated in placental tissues of patients with preeclampsia (PE). The aim this study was to explore the effect on trophoblast cells as well its acting mechanism. We disrupted or overexpressed HTR-8/SVneo and JEG-3 detected associated cellular functional changes by MTT, flow cytometry, Transwell experiment, scratch experiment. results showed overexpression significantly increased proliferation, invasion, migration, EMT process cells. Interfering...
Liddle syndrome, an autosomal dominant form of monogenic hypertension, has been regarded as a rare disorder, which leads to many syndrome patients being misdiagnosed and experiencing severe complications at early age. Little is known about the prevalence syndrome. In this study, authors investigated confirmed by genetic testing among young hypertension undetermined causes in China. A total 330 hypertensive aged 14 40 years after exclusion common secondary were enrolled serum potassium...
Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent with anti-inflammatory and immunomodulatory properties used in the treatment of acute lung injury (ALI). However, ALI using exosomes derived from HUC-MSCs (HUC-MSC-exos) primed interferon-gamma (IFN-γ-exos) has not been described. This study investigated effects IFN-γ-exos on ALI.
Triple‑negative breast cancer (TNBC) is a highly aggressive tumour subtype associated with poor prognosis. The function of leucine‑rich repeat‑containing protein 15 (LRRC15), member the repeat superfamily, in TNBC has not yet been elucidated. aim this study was to identify combined role LRRC15 and Wnt/β‑catenin signalling pathway development TNBC. expression tissues analysed using data from Cancer Genome Atlas. Cell migration invasion assays were conducted proteins via western blotting....
Heme oxygenase-1 (HO-1) is a stress-inducible rate-limiting enzyme in heme degradation that confers cytoprotection against oxidative injury and performs vital function the maintenance of cell hemostasis. Increasing numbers reports have indicated mycoplasma-derived membrane lipoproteins/lipopeptides, such as macrophage-activating lipopeptide-2 (MALP-2), agents stimulate immune system by producing various inflammatory mediators, cytokines cyclooxygenase 2 (COX-2), which play roles pathogenesis...
Catalases are a large group of enzymes that decompose hydrogen peroxide to oxygen and hydrogen, have been applied widely in numerous areas. Bacillus subtilis ATCC 6051a is well-known host strain for high level secretion heterologous peptides. However, the application was seriously hampered by insufficient transformation efficiency. In this study, D-xylose inducible comK integrated into genome B. 6051a, generating 164S, mutant owns efficiency 1 000-fold higher than its parent strain, thus...
Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to one critical factors lower production cost system. In this study, key regulators that affect cell growth and content was confirmed analyzed. S17-3, a mutant E. strain derived from S17-1, found able achieve high density when expressing NphaCAB with plasmid pBhya-CAB. Whole genome sequencing S17-3...
This study explored the effect of focal cerebral contusion on expression ApoE and S-100, its significance in determining time brain injury. Based a rat model contusion, immunohistochemistry was used to analyze expressions S-100 at different points after Thirty minutes following protein significantly increased cortex neurons (P < 0.01), 0.001) elevated 2 h contusion. Over time, number positively expressing cells gradually increased. Three days injury, widely distributed throughout tissue...
Traumatic brain injury (TBI) is a recognized global public health problem. However, there are still limitations in the available therapeutic approaches and lack of clinically effective drugs. Therefore, an in-depth exploration secondary pathological mechanism TBI identification new drugs urgently needed. Cannabidiol (CBD), component derived from cannabis plant, has potential effects on neurological diseases received increasing attention. few reports CBD intervention patients exist. Here, we...
Objective To investigate the effects of glucagon like peptide 1(GLP-1)agonist on myocardial hypoxia reoxygenation injury and its molecular mechanism. Methods H9C2 cells were divided into control group, reoxygenation(H/R)group, H/R+ GLP-1 GLP-1+ phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 group. The cell proliferation activity, apoptosis rate, enzyme contents in medium expressions apoptosis-related genes detected. After animal model ischemia reperfusion injury(I/R)was...
Delivery of therapeutic peptides or proteins into tissues is severely limited by the size and biochemical properties molecules. Protein transduction domain (PTD)-mediated cargo represents a novel promising strategy to deliver biologically active in vivo. The first PTD was identified from HIV-1 transactivating transcriptional activator protein Tat 1988. Since then, other PTDs have also been identified, including third α-helix antennapedia homeotic transcription factor synthetic peptide...
对2017年苏州大学附属儿童医院内分泌遗传代谢科和重症医学科收治的2例糖尿病酮症酸中毒危重患儿的临床表现、实验室检查及抢救治疗过程进行回顾性分析。例1，女，13岁5个月，诊断1型糖尿病、酮症酸中毒、急性肾损伤(3期)；例2，女，2个月8 d，诊断新生儿糖尿病、酮症酸中毒、高渗高血糖综合征，予常规补液、胰岛素降糖治疗效果不佳，行连续性血液透析滤过治疗后缓解；为明确病因，行基因检测，例2检出KCNJ11基因存在c.602G>A(p.R201H)杂合突变。提示对于儿童糖尿病酮症酸中毒危重患者，在常规补液、胰岛素降糖治疗效果不佳，合并其他器官功能损伤及顽固性酸中毒时，积极行连续性血液透析滤过治疗，可获得显著效果。
Abstract Background: Xylan is the second most abundant polysaccharide biomass on earth, polymers have a backbone of β-1,4-linked xylose residues with various side-chain substitutions, such as arabinose, acetic acid, glucuronic and other esterified groups, thus, removal arabinose side groups by α-L-arabinofuranosidases helpful in industrial processes involving xylan treatment. Bacillus subtilis ATCC 6051a known for its excellent capacity secretory production recombinant peptides, however,...