3-Fucosyllactose (3-FL) is an important oligosaccharide and nutrient in breast milk that can be synthesized microbial cells by α-1,3-fucosyltransferase (α-1,3-FucT) using guanosine 5'-diphosphate (GDP)-l-fucose lactose as substrates. However, the catalytic efficiency of known α-1,3-FucTs from various sources was limited due to their low solubility. To enhance production 3-FL, efficiencies were evaluated Bacillus subtilis (B. subtilis) chassis had been endowed with a heterologous synthetic...

Hydrogen gas is a major biofuel and metabolized by wide range of microorganisms. Microbial hydrogen production catalyzed hydrogenase, an extremely complex, air-sensitive enzyme that utilizes binuclear nickel-iron [NiFe] catalytic site. Production engineering recombinant [NiFe]-hydrogenases in genetically-tractable organism, as with metalloprotein complexes general, has met limited success due to the elaborate maturation process required, primarily absence oxygen, assemble center functional...

Although rice husk (RH) has been used as a compost additive, the microbes present in RH are not well understood. In this study, we explored physicochemical parameters, microbial population and functional succession to better understand how different levels (T0: 0; T1: 20%; T2: 30%) of affected performance cow manure composting. Higher thermophilic temperatures (70 °C) higher-quality with respect pH, C/N ratio, particle-size distribution other characteristics were obtained addition 20% RH....

ABSTRACT Microorganisms growing near the boiling point have enormous biotechnological potential but only recently molecular engineering tools become available for them. We engineered hyperthermophilic archaeon Pyrococcus furiosus , which grows optimally at 100°C, to switch its end products of fermentation in a temperature-controlled fashion without need chemical inducers. The recombinant strain (LAC) expresses gene ( ldh ) encoding lactate dehydrogenase from moderately thermophilic...

The cytoplasmic hydrogenase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NADP(H)-dependent heterotetrameric enzyme that contains a nickel-iron catalytic site, flavin, and six iron-sulfur clusters. It has potential utility in range bioenergy systems vitro, but major obstacle its use generating sufficient amounts. We have engineered P. to overproduce SHI utilizing recently developed genetic system. In overexpression (OE-SHI) strain, transcription four-gene operon was under...

3-Ketosteroid-Δ1-dehydrogenase (KstD) is a key enzyme in the metabolic pathway for chemical modifications of steroid hormones. Only few KstDs have thus far been characterized biochemically and applied production steroidal pharmaceutical intermediates. Three KstDs, KstD1, KstD2, KstD3, were identified Mycobacterium neoaurum DSM 1381, they shared up to 99, 85 97% amino acid identity with previously reported respectively. In this paper, from M. 1381 investigated exemplified their potential...

Abstract Background 9α-hydroxyandrost-4-ene-3,17-dione (9-OHAD) is a significant intermediate for the synthesis of glucocorticoid drugs. However, in process phytosterol biotransformation to manufacture 9-OHAD, product degradation, and by-products restrict 9-OHAD output. In this study, construct stable high-yield producer, we investigated combined strategy blocking Δ 1 ‑dehydrogenation regulating metabolic flux. Results Five 3-Ketosteroid-Δ -dehydrogenases (KstD) were identified Mycobacterium...

The first quantum fractal discovered in physics is the Hofstadter butterfly. It stems from large external magnetic fields. We discover instead a new class of non-Hermitian fractals (NHQFs) emerging coupled Hatano-Nelson models on tree lattice absence any Based analytic solutions, we are able to rigorously identify self-similar recursive structures energy spectrum and wave functions. prove that complex NHQFs bears resemblance Mandelbrot set theory. self-similarity rooted interplay between...

The present study investigated the effects of bedding material (BM) waste on physicochemical properties, organic matter (OM) degradation, microbial community structure and metabolic function during composting. results showed that (CK-0, S1-40%, S2-25%) optimized composting conditions for lignocellulose OM biodegradation. highest degradation humic substance (HS) synthesis rates were observed in 40% BM addition group. Firmicutes was more abundant groups, whereas Proteobacteria group without...

Laccases are industrially relevant enzymes that have gained great biotechnological importance. To date, most of fungal and mesophilic origin; however, from extremophiles possess an even greater potential to withstand industrial conditions. In this study, we evaluate the a recombinant spore-coat laccase thermoalkaliphilic bacterium Bacillus sp. FNT (FNTL) biodegrade antibiotics tetracycline, β-lactams, fluoroquinolone families. This extremozyme was previously characterized as being...

Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward reduction. Herein, we engineer TkHDCR to generate an electro-responsive considering safety and convenience. To achieve this purpose, recombinant Escherichia coli overexpression system established. The formate dehydrogenase...

Abstract Background Steroid drugs are essential for disease prevention and clinical treatment. However, due to intricated steroid structure, traditional chemical methods rarely implemented into the whole synthetic process generating intermediates. Novel drug precursors their ideal bacterial strains industrial production have yet be developed. Among these, 9,21-dihydroxy-20-methyl-pregna-4-en-3-one (9-OH-4-HP) is a novel precursor, suitable synthesis of corticosteroids. In this study,...

Escherichia coli does not produce n-butanol naturally, but can be butanologenic when related enzymes were expressed using inducible elements on plasmids. In this study we attempted to confer E. strain capability of automatic excretion the chemical by employing a native anaerobic promoter. Also, novel DNA kit was designed for PCR preparation linear fragments perform modification. The is primarily composed two mother vectors, co-transformation DNAs into simultaneously introduce butanol...

The effects of Lysinibacillus sp. LF-N1 and Penicillium oxalicum DH-1 inoculants (LFPO group) on compost succession the microbial dynamic structure co-composting wheat straw cow manure composting were investigated. contributed to longer thermophilic stages, higher temperatures (62.8 °C) lower diversity in LFPO treatment compared control group (CK). Moreover, inoculation increased germination index accelerated organic matter lignocellulose degradation compost. Microbial analysis confirmed...

Soluble hydrogenase I (SHI) from the hyperthermophilic archaeon Pyrococcus furiosus is a heterotetrameric [NiFe] that catalyzes reversible reduction of protons by NADPH into hydrogen gas (H 2 ). Here, authors expressed four αβγδ subunits SHI encoded one gene cluster in another archaeon, Thermococcus kodakarensis KOD1, which uses its maturation apparatus without coexpression native P. endopeptidases (maturation proteases). The overexpression T. resulted more than 1200‐fold enhancement...